scholarly journals Experimental infection of dogs with Echinococcus granulosus (Batsch, 1986) from protoscoleces of donkey's hydatid cyst

2004 ◽  
Vol 28 (1) ◽  
pp. 15-21
Author(s):  
Raad Harbi Rahif ◽  
Amal Hassan Atae

This study was conducted, for the 1st time in Iraq, to infect 5 puppies, 1 - 5 month old, with protoscoleces of hydatid cyst isolated from the liver of donkey, slaughtered at zoo abattoir of Al-Zawra park in Baghdad, at a dose rate of 10000 protoscoieces i puppy (n=2) and 20000 protoscoleces / puppy (n=3) for 35 and 40 days post infection. The dogs were killed and the developed Echinococcus granulosus were isolated from the small intestine. Results of the study revealed  variation in the period of maturation of the collected cestodes, and the mean total length of the mature worms at 35 days PI was 4.53+ 0.24 mm, and for those at 40 days PI was 7+ 0.20 mm, whereas for the gravid ones at the same age of the latter was 8.57 + 0.25 mm. Results found that infection rate with E. granulosus, which their strobilae composed from 4 proglottids at 35 days PI, was 29.03%, and with those, which their strobilae composed from 5 proglottids at 40 days PI, the rate was 0.76%. It was found that the mean length of the gravid proglottid equal to or longer than half the total worm length with a ratio of 1.77 + 0.05 for those at 35 days of age, and 2.2 + 0.35 and 1.93 + 0.01 for mature and gravid cestodes at the age of 40 days.

2005 ◽  
Vol 29 (1) ◽  
pp. 64-75
Author(s):  
Raad Harbi Rahif

The study highligthened on the effect of the field enviromental & controlledtempratures in the experimental infection with Echinococcus granulosus indogs, n = 6 local dogs, at the age of 1.5-3 months, were used . Each dog wasdosed with 8658 protoscoleces with 92.78% viability for 28 and 33 days postinfection (PI). Animals were subdivided into 2groups. Results denoted that thelow enviromental temperatures retarded the growth and development of E.granulosus.All tapeworms, isolated from the small intestine of dogs housedunder controlled temperatures, with the average of 21.85ºc(group1),had 3proglottids,whereas those isolated from dogs housed under field temperatures,with the average of 11.12ºc (group2) ,had only 2 proglottids.The average totallength of E. granulosus ,at the age of 28 days, isolated fromgroup1,was1.70±0.24mm,longer than those that isolated from group2,with theaverage length of 1.13±0.06mm.The average total length of E.granulosus ,at theage of 33 days , isolated from group 1 and 2 ,was 2.68±0.35 mm and 1.67±0.21,respectively . Significant statistical differences (p<0.05) were found in theaverage total length of E.granulosus isolated from both groups at different ages.Echinococcus granulosus isolated from group1 were found mature more thanthose isolated from group2,and the number of rostellar hooks of the former werealso more than those of the 2nd group, the number of rostellar hooks were, at theage of 33 days,were34.53±2.19 and29.47±0.62 for the 1st and 2nd gr


Parasitology ◽  
2000 ◽  
Vol 121 (5) ◽  
pp. 501-505 ◽  
Author(s):  
V. R. SOUTHGATE ◽  
L. A. TCHUEM TCHUENTÉ ◽  
A. THÉRON ◽  
J. JOURDANE ◽  
A. LY ◽  
...  

The vectorial capacity of Biomphalaria pfeifferi from Ndiangue, Senegal, was investigated with an allopatric isolate of Schistosoma mansoni from Nkolbisson, Cameroon. The snail infection rate after exposure to a single miracidium per snail (MD1) was 56·3%, and 91·6% for snails exposed to 5 miracidia per snail (MD5). The minimum pre-patent period was 21 days. The mean total cercarial production for the MD1 group was 18511 cercariae per snail, and 9757 cercariae for the MD5 group. The maximum production of cercariae for 1 day was 4892 observed in a snail from the MD1 group at day 43 post-infection. The mean longevity of snails was higher in group MD1 (88 days p.i.) than in group MD5 (65 days p.i.). The chronobiological emergence pattern revealed a circadian rhythm with one shedding peak at mid-day. Comparisons are made with the vectorial capacity of the sympatric combination of B. pfeifferi Senegal/S. mansoni Senegal.


2018 ◽  
Vol 5 (3) ◽  
pp. 983
Author(s):  
Ghada Morshed ◽  
Tamer El-Gaabary

Background: Laparoscopic splenectomy is feasible, effective, and safe with low morbidity rates, faster recovery when compared with patients who undergo open splenectomy. The aim of this study was to describe a new technique for laparoscopic partial splenectomy to decrease blood loss.Methods: This study started from May 2015 till April 2017. This retrospective study included total 20 adults containing 12 females, ten with hydatid cyst of the spleen with positive serological tests for Echinococcus granulosus (Indirect hemagglutination (IHA)) was used, and two cases with congenital splenic cysts with negative serology for Echinococcus granulosus and eight males with hydatid cyst positive serological tests for Echinococcus granulosus.Results: The mean time for laparoscopic resection was 150 minutes (range from 90 to 200 min). No bleeding, no postoperative pancreatic fistula or diaphragmatic injury. No conversion was necessary. The mean hospital stay was 4 days (range from 2 to 6 days). None of the patients needed postoperative blood transfusion. No post-splenectomy infections. Three weeks and after 6 months postoperatively, US Doppler was done and found normal vascularisation of the splenic remnant.Conclusions: Clipless, Sutureless laparoscopic partial splenectomy is feasible and safe. This surgical procedure offers advantages of decrease blood loss and preservation of splenic tissue, reducing the risk of post-splenectomy sepsis.


2011 ◽  
Vol 35 (2) ◽  
pp. 11-13
Author(s):  
Amall Hassen Atia

An experimental infection of 3 dogs with protoscoleces of human origin were carried out Hydatid cyst was surgical removed from 26 years old female. On autopsy all dogs were found not harbor any Echinococcus granulosus worms Infection with the metacestode stage in unusual intermediate hosts failure for procreation which do not play a role in the transmission cycle in Iraq. In conclusions: the reason could be related between the host and chemical composition of hydatid fluid failure of induces infection


2013 ◽  
Vol 55 (2) ◽  
pp. 133-136 ◽  
Author(s):  
Vitor Luís Tenório Mati ◽  
Hudson Alves Pinto ◽  
Alan Lane de Melo

In order to better understand the biology of Centrocestus formosanus in a definitive host model, mice of Swiss and AKR/J strains were experimentally infected with 100 metacercariae of the parasite. Fourteen days post-infection, the rodents were killed and adult trematodes were recovered from the small intestine. The percentage of parasite recovery from AKR/J mice (11.4%) was significantly higher than that from Swiss mice (5.3%). Moreover, trematodes recovered from the AKR/J strain were more developed and had greater fecundity. Peculiarities concerning the mice’s immune system could explain the difference in susceptibility and in worm development seen in the present study. The data obtained confirm that mice are susceptible to infection with C. formosanus and indicate that the AKR/J strain provides a more favorable environment for parasite development.


1957 ◽  
Vol 35 (3) ◽  
pp. 195-202 ◽  
Author(s):  
M. A. A. Vonk ◽  
L. W. McElroy ◽  
R. T. Berg

Four treatments, involving differences in method of feeding and interval between last feed and slaughter, were employed in a study of the effect of dietary chlortetracycline on protease, amylase, and cellulase activity in the intestinal and cecal contents of 16 pairs of weanling pigs. Most consistent results were obtained with six pairs which were limited pair-fed except for the final feeding during which feed was available ad libitum for a 4-hour period ending 18 hours before slaughter. The mean total activities of all three hydrolases in the contents of the small intestines and of the ceca of the antibiotic-fed animals of these six pairs were significantly greater than in those of the control animals. Expressed as activity per gram dry matter of intestinal contents, significantly higher values for protease and amylase, but not for cellulase, were observed in the pigs that had received chlortetracycline. When the combined results obtained from all 16 pairs of the experimental animals were analyzed, the results showed that on a basis of activity per gram dry matter of intestinal contents, ingested chlortetracycline was associated with significant increases in amylase and cellulase but not in protease activity. Protease, amylase, and cellulase activities per gram dry matter of cecal contents were higher for pigs fed the antibiotic than for their controls. The mean wet weight of the empty small intestine and the mean dry weight of the mucosa scraped from the anterior 3-meter section of the small intestine were lower for the chlortetracycline-fed animals, but the differences were not statistically significant.


2017 ◽  
Vol 31 (1) ◽  
pp. 66-70
Author(s):  
Raheleh Rafiei Sefiddashti ◽  
Seyedeh Maryam Sharafi ◽  
Soltan Ahmad Ebrahimi ◽  
Lame Akhlaghi ◽  
Ali Moosavi ◽  
...  

2008 ◽  
Vol 82 (2) ◽  
pp. 97-100 ◽  
Author(s):  
N.K. Sasmal ◽  
T.K. Pahari ◽  
R. Laha

AbstractThe possible role of the cockroachPeriplaneta americanain the transmission ofToxocara caniseggs and larvae via faeces and tissue migration was studied. Cockroaches fed with 3 × 105and 5 × 105embryonated eggs were found to harbour viable eggs and larvae from days 1 to 5 post-infection (DPI). At necropsy on 5 DPI, eggs and larvae were also recovered from the rectal contents but not from the tissues of cockroaches. In addition patent infections were established in pups fed on infected faeces of cockroaches, with eggs first appearing in the faeces of pups at 38 DPI. Adult worms ofT. caniswere also recovered at necropsy. Therefore the importance of cockroaches as good mechanical disseminators of ascarid eggs, especiallyT. canis, is discussed.


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