Optimization of fermentation parameters for enhanced APHE production from Streptomyces griseocarneus through submerged fermentation

Sophorolipids (SLs) are glycolipidic biosurfactants suitable for various biological and physicochemical applications. The nonedible Jatropha oil has been checked as the alternative raw material for SL synthesis usingC. bombicola(ATCC22214). This is useful towards lowering the SL production cost. Through optimization of fermentation parameters and use of resting cell method, the yield 15.25 g/L could be achieved for Jatropha oil derived SL (SLJO) with 1% v/v oil feeding. The synthesized SL displayed good surfactant property. It reduced the surface tension of distilled water from 70.7 mN/m to 33.5 mN/m with the Critical Micelle Concentration (CMC) value of 9.5 mg/L. Keeping the prospective use of the SL in mind, the physicochemical properties were checked along with emulsion stability under temperature, pH stress, and in hard water. Also antibacterial action and stain removal capability in comparison with commercial detergent was demonstrated. SLJO enhanced the detergent performance. Based on the results, it can be said that SLs have utility as fabric cleaner with advantageous properties such as skin friendly nature, antibacterial action, and biodegradability. Therefore SLs are potential green molecules to replace synthetic surfactants in detergents so as to reduce harm caused to environment through detergent usage.

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Optimization of Fermentation Parameters for Laccase Production by a Novel Deuteromycete Fungus Myrothecium Verrucaria NF-05 Using Response Surface Methodology

Proceedings of the 2nd International Conference on Civil, Materials and Environmental Sciences ◽

10.2991/cmes-15.2015.118 ◽

2015 ◽

Cited By ~ 2

Author(s):

Dan Zhao ◽

Yanyang Sun ◽

Renpeng Du ◽

Li Zhang ◽

Jingping Ge

Keyword(s):

Response Surface Methodology ◽

Response Surface ◽

Laccase Production ◽

Myrothecium Verrucaria ◽

Fermentation Parameters ◽

Optimization Of Fermentation

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Improved Production of Sublancin 168 Biosynthesized byBacillus subtilis168 Using Chemometric Methodology and Statistical Experimental Designs

BioMed Research International ◽

10.1155/2015/687915 ◽

2015 ◽

Vol 2015 ◽

pp. 1-9 ◽

Cited By ~ 1

Author(s):

Shengyue Ji ◽

Weili Li ◽

Haiyun Xin ◽

Shan Wang ◽

Binyun Cao

Keyword(s):

Soybean Meal ◽

Incubation Temperature ◽

Optimal Combination ◽

Fermentation Condition ◽

Box Behnken Design ◽

Steepest Ascent ◽

Burman Design ◽

Fermentation Parameters ◽

Optimization Of Fermentation ◽

Plackett Burman Design

Sublancin 168, as a distinct S-linked antimicrobial glycopeptide produced byBacillus subtilis168, is effective in killing specific microorganisms. However, the reported yield of sublancin 168 is at a low level of no more than 60 mg from 1 L fermentation culture ofB. subtilis168 by using the method in the literature. Thus optimization of fermentation condition for efficiently producing sublancin 168 is required. Here, Box-Behnken design was used to determine the optimal combination of three fermentation parameters, namely, corn powder, soybean meal, and temperature that were identified previously by Plackett-Burman design and the steepest ascent experiment. Subsequently, based on the response surface methodology, the quadratic regression model for optimally producing sublancin 168 was developed, and the optimal combination of culture parameters for maximum sublancin 168 production of 129.72 mg/L was determined as corn powder 28.49 g/L, soybean meal 22.99 g/L, and incubation temperature 30.8°C. The results showed that sublancin 168 production obtained experimentally was coincident with predicted value of 125.88 mg/L, and the developed model was proved to be adequate, and the aim of efficiently producing sublancin 168 was achieved.

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