Diagnostic value of Rf1 gene molecular markers in sunflower

Background. Modern production of sunflower seeds is currently based on the cultivation of high-yielding heterotic F1 hybrids from cross-breeding of lines with cytoplasmic male sterility (CMS) of PET1-type and fertility restorer lines. The paternal parent serves as a donor of the nuclear Rf1 gene functional allele, which is responsible for pollen fertility restoration in F1 plants. The detection of carriers of the Rf1 locus recessive and dominant alleles using diagnostic molecular markers accelerates breeding of female and male parental lines for creating hybrids. Materials and methods. The material for the study included 75 lines of various origins from the VIR sunflower genetic collection as well as hybrids from crosses of VIR 116A sterile line with fertile lines differing in the type of cytoplasm (fertile or sterile) and the presence of molecular markers, most of which were linked to the Rf1 locus. For marker validation, two different approaches were used: either by analyzing associations between the ability of a line to restore pollen fertility and the presence of molecular markers in its genotype, or by estimating recombination frequency between the Rf1 locus and marker loci in four segregating hybrid populations. Results. According to the obtained results, no markers demonstrated 100% efficiency in the analysis of the sample of genotypes. The ORS511 marker was most frequently observed among the lines presumably carrying the dominant allele Rf1. Pollen fertility of F1 hybrids from interline crossings was 89-99%. The segregation for fertility/sterility in F2 fitted the theoretical ratio of 3:1 expected in case of the monogenic control of the trait. The markers HRG01, HRG02 and ORS511 were linked to the fertility restoration trait, with recombination rates between Rf1 locus and markers varying in different cross combinations. The analysis of VIR 116А × VIR 740 and VIR 116А × RIL 130 hybrids showed that among the marker loci studied, the ORS511 was closest to the Rf1 locus Rf1 (recombination frequency of 2.2 and 3.3%, respectively). The recombination rate between the Rf1 and ORS511 loci equaled 7.5% in the cross VIR 116А × VIR 210 and 8.9% in VIR 116 × VIR 195. Conclusion. The markers ORS511, HRG01 and HRG02 are the most efficient for the identification of alleles of the Rf1 gene and for the marker assisted selection in hybrid populations produced involving sunflower lines from the VIR collection.

Download Full-text

Mutator-Induced Mutations of the rf1 Nuclear Fertility Restorer of T-Cytoplasm Maize Alter the Accumulation of T-urfl3 Mitochondrial Transcripts

Genetics ◽

10.1093/genetics/143.3.1383 ◽

1996 ◽

Vol 143 (3) ◽

pp. 1383-1394

Author(s):

Roger P Wise ◽

Carren L Dill ◽

Patrick S Schnable

Keyword(s):

Pollen Fertility ◽

Fertility Restoration ◽

Mitochondrial Rna ◽

Induced Mutations ◽

Male Sterile ◽

Fertility Restorer ◽

Restorer Genes ◽

Mitochondrial Transcripts ◽

Ecori Restriction ◽

Fertility Restorer Genes

Abstract Dominant alleles of the rf1 and rf2 nuclear-encoded fertility restorer genes are necessary for restoration of pollen fertility in T-cytoplasm maize. To further characterize fertility restoration mediated by the Rf1 allele, 123,500 gametes derived from plants carrying the Mutator transposable element family were screened for rf1-mutant alleles (rf1-m) Four heritable rf1-m alleles were recovered from these populations. Three rf1-m alleles were derived from the progenitor allele Rf1-IAl53 and one was derived from Rf1-Ky21. Cosegregation analysis revealed 5.5- and 2.4kb Mu1-hybridizing EcoRI restriction fragments in all of the male-sterile and none of the male-fertile plants in families segregating for rf1-m3207 and rf1-m3310, respectively. Mitochondrial RNA gel blot analyses indicated that all four rf1-m alleles in male-sterile plants cosegregated with the altered steady-state accumulation of 1.6 and O.6-kb T-urf13 transcripts, demonstrating that these transcripts are Rf1 dependent. Plants carrying a leaky mutant, rf1-m7323, revealed variable levels of Rf1-associated, T-urf13 transcripts and the degree of pollen fertility. The ability to obtain rf1-m derivatives from Rf1 indicates that Rf1 alleles produce a functional gene product necessary for the accumulation of specific T-urf13 transcripts in T-cytoplasm maize.

Download Full-text

Molecular markers in identification of sunflower pollen fertility restorer genes

Russian Agricultural Sciences ◽

10.3103/s1068367409060020 ◽

2009 ◽

Vol 35 (6) ◽

pp. 367-370 ◽

Cited By ~ 3

Author(s):

I. N. Anisimova ◽

V. A. Gavrilova ◽

V. T. Rozhkova ◽

G. I. Timofeeva ◽

M. A. Tikhonova

Keyword(s):

Molecular Markers ◽

Pollen Fertility ◽

Fertility Restorer ◽

Restorer Genes ◽

Pollen Fertility Restorer ◽

Fertility Restorer Genes

Download Full-text

A single nucleotide substitution in the coding region of Ogura male sterile gene, orf138, determines effectiveness of a fertility restorer gene, Rfo, in radish

Molecular Genetics and Genomics ◽

10.1007/s00438-021-01777-y ◽

2021 ◽

Author(s):

Hiroshi Yamagishi ◽

Megumi Jikuya ◽

Kanako Okushiro ◽

Ayako Hashimoto ◽

Asumi Fukunaga ◽

...

Keyword(s):

Male Sterility ◽

Nucleotide Substitution ◽

Fertility Restoration ◽

Type A ◽

Restorer Gene ◽

Single Nucleotide Substitution ◽

Coding Region ◽

Fertility Restorer ◽

Single Nucleotide ◽

Fertility Restorer Gene

AbstractCytoplasmic male sterility (CMS) observed in many plants leads defect in the production of functional pollen, while the expression of CMS is suppressed by a fertility restorer gene in the nuclear genome. Ogura CMS of radish is induced by a mitochondrial orf138, and a fertility restorer gene, Rfo, encodes a P-type PPR protein, ORF687, acting at the translational level. But, the exact function of ORF687 is still unclear. We found a Japanese variety showing male sterility even in the presence of Rfo. We examined the pollen fertility, Rfo expression, and orf138 mRNA in progenies of this variety. The progeny with Type H orf138 and Rfo showed male sterility when their orf138 mRNA was unprocessed within the coding region. By contrast, all progeny with Type A orf138 were fertile though orf138 mRNA remained unprocessed in the coding region, demonstrating that ORF687 functions on Type A but not on Type H. In silico analysis suggested a specific binding site of ORF687 in the coding region, not the 5′ untranslated region estimated previously, of Type A. A single nucleotide substitution in the putative binding site diminishes affinity of ORF687 in Type H and is most likely the cause of the ineffectiveness of ORF687. Furthermore, fertility restoration by RNA processing at a novel site in some progeny plants indicated a new and the third fertility restorer gene, Rfs, for orf138. This study clarified that direct ORF687 binding to the coding region of orf138 is essential for fertility restoration by Rfo.

Download Full-text

Diagnostic value of molecular markers for Lr genes and characterization of leaf rust resistance of German winter wheat cultivars with regard to the stability of vertical resistance

European Journal of Plant Pathology ◽

10.1007/s10658-011-9778-2 ◽

2011 ◽

Vol 130 (4) ◽

pp. 559-575 ◽

Cited By ~ 12

Author(s):

Albrecht Serfling ◽

Ilona Krämer ◽

Volker Lind ◽

Edgar Schliephake ◽

Frank Ordon

Keyword(s):

Molecular Markers ◽

Winter Wheat ◽

Rust Resistance ◽

Leaf Rust Resistance ◽

Diagnostic Value ◽

Wheat Cultivars ◽

Vertical Resistance ◽

Winter Wheat Cultivars ◽

The Stability

Download Full-text

Construction of a Genetic Linkage Map in Tetraploid Species Using Molecular Markers

Genetics ◽

10.1093/genetics/157.3.1369 ◽

2001 ◽

Vol 157 (3) ◽

pp. 1369-1385 ◽

Cited By ~ 2

Author(s):

Z W Luo ◽

C A Hackett ◽

J E Bradshaw ◽

J W McNicol ◽

D Milbourne

Keyword(s):

Molecular Markers ◽

Linkage Map ◽

Recombination Frequency ◽

Simulated Data ◽

Likelihood Estimation ◽

Lod Score ◽

Data Set ◽

Independent Segregation ◽

The Em Algorithm ◽

Small Set

Abstract This article presents methodology for the construction of a linkage map in an autotetraploid species, using either codominant or dominant molecular markers scored on two parents and their full-sib progeny. The steps of the analysis are as follows: identification of parental genotypes from the parental and offspring phenotypes; testing for independent segregation of markers; partition of markers into linkage groups using cluster analysis; maximum-likelihood estimation of the phase, recombination frequency, and LOD score for all pairs of markers in the same linkage group using the EM algorithm; ordering the markers and estimating distances between them; and reconstructing their linkage phases. The information from different marker configurations about the recombination frequency is examined and found to vary considerably, depending on the number of different alleles, the number of alleles shared by the parents, and the phase of the markers. The methods are applied to a simulated data set and to a small set of SSR and AFLP markers scored in a full-sib population of tetraploid potato.

Download Full-text

Inheritance of fertility restoration of A4 cytoplasm in pearl millet [Pennisetum glaucum (L.) R. Br.]

Indian Journal of Genetics and Plant Breeding (The) ◽

10.31742/ijgpb.80.1.8 ◽

2020 ◽

Vol 80 (01) ◽

Author(s):

J. Jorben ◽

S. P. Singh ◽

C. Tara Satyavathi ◽

S. Mukesh Sankar ◽

Jayant S. Bhat ◽

...

Keyword(s):

Pearl Millet ◽

Pollen Fertility ◽

Seed Set ◽

Pennisetum Glaucum ◽

Fertility Restoration ◽

Duplicate Genes ◽

New Delhi ◽

Regional Centre ◽

Fertility Restorers ◽

Mode Of Inheritance

Present investigation was carried out to study the mode of inheritance of fertility restoration for A4 cytoplasm using pollen fertility and seed set per cent as criterion in determining the fertile and sterile plants. Two CMS lines of A4 cytoplasm were crossed with two fertility restorers generating four F1 crosses, namely, ICMA 99111 x PPMI 1003, ICMA 99111 x PPMI 1087, ICMA 03999 x PPMI 1003 and ICMA 03999 x PPMI 1087, their F2s and backcross generations. All the F1s were completely fertile indicating complete fertility restoration. F2s and backcross generations were evaluated at IARI, New Delhi and IARI Regional Centre, Dharwad during summer 2017 and χ 2 test was applied to test the significance. At both the locations, all the F2 segregating populations fit well into a Mendelian ratio of 15:1 indicating digenic duplicate dominance of fertility restoring genes with χ 2 value of 0.82, 2.90, 0.04, 3.97, 4.86, 4.98, 0.02, 1.26, 3.15, 4.98, 3.15 and 0.02. The F2 hypothesis was verified with the observed frequency of segregating plants fitting well into 3:1 ration with χ 2 value of 5.45, 1.93, 4.93, 0.60, 2.83, 0.44, 4.94, 2.77, 3.33, 0.13, 4.08 and 1.51. It is further confirmation of the findings that fertility restoration is indeed governed by two duplicate genes. Association between pollen fertility and seed set per cent was significant and positive.

Download Full-text

Molecular markers applying in forest trees gene pool conservation

Glasnik Sumarskog fakulteta ◽

10.2298/gsf0999101m ◽

2009 ◽

pp. 101-113

Author(s):

Jelena Milovanovic ◽

Mirjana Sijacic-Nikolic

Keyword(s):

Population Genetics ◽

Molecular Markers ◽

Gene Flow ◽

Gene Pool ◽

Statistical Power ◽

Forest Trees ◽

Effective Population ◽

Migration Patterns ◽

Geographic Diversity ◽

Marker Loci

Many studies performed during the last years demonstrated the usefulness of neutral molecular markers in the field of conservation and population genetics of forest trees, in particular to understand the importance of migration patterns in shaping current genetic and geographic diversity and to measure important parameters such as effective population size, gene flow and past bottleneck. During the next years, a large amount of data at marker loci or at sequence level is expected to be collected, and to become excellent statistical power for the assessment of biological and evolutionary value.

Download Full-text

Study of the possibilities of using sunflower lines with different colours of seeds to create poultry feed

Helia ◽

10.1515/helia-2021-0016 ◽

2021 ◽

Vol 0 (0) ◽

Author(s):

Katerina Vedmedeva ◽

Tatiana Machova

Keyword(s):

Pollen Fertility ◽

Animal Feed ◽

Fertility Restoration ◽

Emerging Market ◽

Pollen Sterility ◽

Wild Birds ◽

Hybrid Breeding ◽

Poultry Feed ◽

Human Consumption ◽

Seed Colour

Abstract Sunflower is used for the production of oil, confectionery and animal feed. Birds are very fond of sunflowers and can be pests of sunflower crops, and are consumers of seeds. Sunflower poultry feed is an emerging market that determines the direction of breeding. Its development is based on the determination of bird preferences and the available variety of sunflower lines. This is what our research is devoted to. Experimental feeding of chickens with a mixture of sunflower seeds of different colours was carried out. Chickens have been found to prefer contrasting striped seeds with white and dark stripes more than others. The white colour of the seeds was eaten less than others. Studies of the genetics of sunflower colour allow us to distinguish two groups of lines by seed colour. The first has white seeds with the EwEwPP genotype, suitable for use in human confectionery and more protected from being eaten by wild birds in the fields. The second is striped seeds with the EstrEstrPP genotype, which can be fed whole seeds to birds. Donors of seed colour traits and other traits important for hybrid breeding were selected from the evaluated collection of sunflower lines. InK1039 line is a donor of small striped seeds and pollen fertility restoration. InK1587 line is a sterility fixer and donor of striped and early maturing seeds. To create hybrids with white seeds for human consumption and thus more resistant to ingestion by wild birds, white seed donors were isolated with KG9 to restore pollen fertility and I2K2218 in a pollen sterility fixer.

Download Full-text