Inheritance of fertility restoration of A4 cytoplasm in pearl millet [Pennisetum glaucum (L.) R. Br.]

Present investigation was carried out to study the mode of inheritance of fertility restoration for A4 cytoplasm using pollen fertility and seed set per cent as criterion in determining the fertile and sterile plants. Two CMS lines of A4 cytoplasm were crossed with two fertility restorers generating four F1 crosses, namely, ICMA 99111 x PPMI 1003, ICMA 99111 x PPMI 1087, ICMA 03999 x PPMI 1003 and ICMA 03999 x PPMI 1087, their F2s and backcross generations. All the F1s were completely fertile indicating complete fertility restoration. F2s and backcross generations were evaluated at IARI, New Delhi and IARI Regional Centre, Dharwad during summer 2017 and χ 2 test was applied to test the significance. At both the locations, all the F2 segregating populations fit well into a Mendelian ratio of 15:1 indicating digenic duplicate dominance of fertility restoring genes with χ 2 value of 0.82, 2.90, 0.04, 3.97, 4.86, 4.98, 0.02, 1.26, 3.15, 4.98, 3.15 and 0.02. The F2 hypothesis was verified with the observed frequency of segregating plants fitting well into 3:1 ration with χ 2 value of 5.45, 1.93, 4.93, 0.60, 2.83, 0.44, 4.94, 2.77, 3.33, 0.13, 4.08 and 1.51. It is further confirmation of the findings that fertility restoration is indeed governed by two duplicate genes. Association between pollen fertility and seed set per cent was significant and positive.

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Identification of new stable and high iron rich fertility restorers in pearl millet

Indian Journal of Genetics and Plant Breeding (The) ◽

10.31742/ijgpb.79.3.4 ◽

2019 ◽

Vol 79 (3) ◽

Author(s):

Tripti . Singhal ◽

S. P. Singh ◽

S. Mukesh Sankar ◽

C. . Bharadwaj ◽

...

Keyword(s):

Pearl Millet ◽

Pennisetum Glaucum ◽

Fertility Restoration ◽

P Value ◽

Environment Interaction ◽

Nutritional Security ◽

High Iron ◽

Genotype Environment Interaction ◽

Promising Line ◽

Fertility Restorers

Biofortification of pearl millet (Pennisetum glaucum (L.) R. Br.) with improved iron (Fe) and zinc (Zn) will have great impact as it is an indispensable component of nutritional security of inhabitants of arid and semi-arid regions. Ten genotypes along with checks were evaluated in RBD in six locations during kharif, 2016 under rainfed conditions. Significant differences were observed in genotype, environment and genotype × environment interaction mean squares for grain Fe and Zn contents, indicating differential nutrient accumulation by the genotypes. The first two principal components obtained in AMMI analysis were significant and cumulatively explained the total variation were 81.47 % for Fe and 73.97 % for Zn. A positive and moderately high correlation (r=0.6) between Fe and Zn contents suggests good prospects of simultaneous improvement for both micronutrients. Among the ten genotypes, PPMI 953 was found to be more stable with high mean Fe (90 ppm) and Zn (59 ppm) contents. On crossing with designated A lines of pearl millet, the line PPMI 953 found to be restorer for A1 system with complete fertility restoration of F1 panicle of the cross, ICMA(1) 863 x PPMI 953 under bagged condition and resulting F1 with 78-84% fertility measured by seed setting % under bag. The F2 individuals showed 9:7 fertility-sterility ratio (χ 2 value=0.002, P value=0.964). The promising line, PPMI 953 may be used as source for further genetic improvement with respect to grain micronutrient content or can be directly used as male parent in development of high iron pearl millet hybrids.

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Seed Production on Detached Culms of Pearl Millet × Elephantgrass Hexaploid Hybrids

HortScience ◽

10.21273/hortsci.31.5.827 ◽

1996 ◽

Vol 31 (5) ◽

pp. 827-828 ◽

Cited By ~ 1

Author(s):

S.C. Schank ◽

D.A. Diz ◽

D.S. Wofford

Keyword(s):

Pearl Millet ◽

Seed Production ◽

Seed Set ◽

Pennisetum Glaucum ◽

Sucrose Solution ◽

Phenotypic Selection ◽

Nutrient Solutions

Five nutrient solutions were evaluated in the greenhouse to determine which solutions would allow detached culms of Pennisetum to produce seed. The genotypes tested originated from the hybridization of Pennisetum glaucum L. (Pearl millet) × P. pennisetum Schum. (elephantgrass). The solutions were water, Hoagland's, sucrose, sucrose + hydroxyquinoline sulfate (HQ), and Hoagland's + sucrose + HQ. Neither the water nor the Hoagland's solution supported high seed set. Although the sucrose solution enhanced seed production, the seeds were low in weight and did not germinate well. The best nutrient solutions were 2% sucrose + 0.02% HQ or Hoagland's + 2% sucrose + 0.02% HQ. The four genotypes used differed substantially in seed production, but all produced seed, with germination >25%. This result indicates that the cut-culm technique is a possible way of getting recurrent restricted phenotypic selection seed in Pennisetum hexaploid hybrids.

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Fertility Restoration and Cytological Studies of Pollen in Pearl Millet (Pennisetum glaucum (L.) R.Br.) Male Sterile and Fertile Lines

International Journal of Plant Breeding and Genetics ◽

10.3923/ijpbg.2008.85.92 ◽

2008 ◽

Vol 2 (2) ◽

pp. 85-92

Author(s):

M. Vetriventh ◽

A. Nirmalakum ◽

K. Ganeshamur ◽

D. Sudhakar ◽

S. Ganapathy ◽

...

Keyword(s):

Pearl Millet ◽

Pennisetum Glaucum ◽

Fertility Restoration ◽

Male Sterile

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A New Source of Yellow Coloration of the Sunflower Plant Top and Its Importance in Breeding

Helia ◽

10.1515/helia-2019-0006 ◽

2019 ◽

Vol 42 (71) ◽

pp. 213-220

Author(s):

V.M. Popov ◽

T.A. Dolhova

Keyword(s):

Genetic Control ◽

Seed Production ◽

Inbred Line ◽

Wild Species ◽

Pollen Fertility ◽

Morphological Traits ◽

Fertility Restoration ◽

Sunflower Plant ◽

Yellow Coloration ◽

Mode Of Inheritance

Abstract Inbred line Mh174B with the yellow coloration of the sunflower plant top was produced by hybridization of annual wild species with cultivated sunflower. The aim research was to evaluate genetic control of yellow coloration of the sunflower plant top in combination with other morphological traits (branchiness, pollen fertility restoration and ray flower coloration). A total of four F2 hybrid combinations of were created: Сх1010А × Mh174B, Сх1012А × Mh174B, Сх2111А × Mh174B, Мх845А × Mh174B. Yellow coloration of the sunflower plant top was shown to follow a monogenic mode of inheritance and, presumably, be controlled by the gene y. This trait is inherited independently from such traits as branchiness, pollen fertility restoration and ray flower coloration. The mainstreams of use of yellow coloration of the sunflower plant top in sunflower breeding and seed production are discussed.

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Identification of stable restorers for diverse cytoplasmic source in Sorghum [Sorghum bicolor (L.) Moench.]

Indian Journal of Genetics and Plant Breeding (The) ◽

10.31742/ijgpb.80.2.14 ◽

2020 ◽

Vol 80 (02) ◽

Author(s):

N. Sandeep ◽

Basavaraj Biradar

Keyword(s):

Cytoplasmic Male Sterility ◽

Sorghum Bicolor ◽

Seed Set ◽

Fertility Restoration ◽

Male Sterile ◽

Commercial Exploitation ◽

M 31 ◽

Fertility Restorers ◽

Sorghum Bicolor L ◽

Sorghum Germplasm

In sorghum [Sorghum bicolor (L.) Moench.], milo is the lone source of cytoplasmic male sterility (CMS) extensively used in production of commercial hybrids. The hazards of such a narrow cytoplasmic base are apparent and consequently new diverse sources like A2, A3, A4, maldandi, VZM and G1 were identified. However, the commercial exploitation of these sources has not been possible because of the difficulty in fertility restoration on these sources. The present study was, therefore, undertaken to test different sorghum germplasm lines for their fertility restoration/maintenance on maldandi and milo cytoplasm. Twenty-five diverse accession were selected from minicore collection and crossed with two male sterile lines viz., 104A (milo) and M 31-2A (maldandi). The resulting F1s were classified as fertility restorers and maintainers based on seed set on bagged ear heads. The restoration studies indicated that 2 lines on maldandi and 6 on milo cytoplasm showed strong fertility restoration (> 90 % seed set).

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Development and validation of CAPS-marker associated with the Rf2 gene in sorghum (Sorghum bicolor (L.) Moench)

PLANT BIOTECHNOLOGY AND BREEDING ◽

10.30901/2658-6266-2021-2-o4 ◽

2021 ◽

Vol 4 (2) ◽

pp. 38-47

Author(s):

E. E. Radchenko ◽

N. V. Alpatieva ◽

Yu. I. Karabitsina ◽

M. K. Ryazanova ◽

E. B. Kuznetsova ◽

...

Keyword(s):

Sorghum Bicolor ◽

Pollen Fertility ◽

Fertility Restoration ◽

Hybrid Breeding ◽

Sequence Variant ◽

Chromosome 2 ◽

Functional Alleles ◽

Ppr Genes ◽

Development And Validation ◽

Fertility Restorers

Background. The development of heterotic hybrids based on cytoplasmic male sterility (CMS) is the leading strategy in breeding sorghum (Sorghum bicolor (L.) Moench). The trait of pollen fertility restoration in forms with CMS A1 (milo), predominantly used in sorghum breeding, is determined by at least two dominant complementary genes Rf1 and Rf2, and also gene Rf5. The development of accessible molecular markers of sorghum Rf genes is highly relevant for hybrid breeding, since they can significantly accelerate the process of creating female sterile forms (A lines), sterility maintainers (B lines) and pollen fertility restorers (R lines).Material and methods. The studied material included 36 sorghum accessions from the VIR collection, which differed by the ability to restore pollen fertility in forms with A1-type CMS. The nucleotide polymorphism of 935 bp fragments of the PPR genes Sobic.002G057050, Sobic.002G054100, and Sobic.002G054200 located at the chromosome 2 was studied.Results. The fragments obtained with the use of a pair of 2459403fw and 2459403 primers were 935 bp long and included parts of three genes: Sobic.002G057050, Sobic.002G054100, Sobic.002G054200. For identifying the sequence variant Sobic.002G057050-1090 associated with the Rf2 gene, Tru9 I restrictase was chosen, which allows obtaining a 572 bp fragment unique for all the studied R lines. Such a marker was found in 10 sorghum lines from West China and Kyrgyzstan, which are widely used in breeding as fertility restorers. The fragment was found neither in three lines with sterile cytoplasm and their fertile analogues, nor in 7 accessions of kafir sorghum, which lacked functional alleles of Rf genes.Conclusions. It has been demonstrated that the marker can be used for selection and checking purity of R and B/A lines. It is also applicable for verifying hybridity of F1 seeds and analyzing hybrid populations from crosses of R lines 924-4, 928-1, 929-3, 931-1, 933-1/6, 1237-3, 1243-2, 1251, 1150-1, F10BC2 with A lines Nizkorosloe 81s, А-83 and А-10598. It may be suggested that the ability to restore pollen fertility in R lines, which lack the marker CAPS- 572, is determined by another Rf gene. The studied 935 bp fragment of Sobic.002G057050 harbours 22 SNP, therefore the development of CAPS-markers for their identification and differentiation can be promising.

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