Medical thermography in evaluation of results of skin testing by prick test method in patients with IgE-mediated allergic diseases

2020 ◽  
Vol 0 (1) ◽  
pp. 76-80
Author(s):  
A.Ye. Bogomolov
Keyword(s):  
Skin Testing ◽  
Allergic Diseases ◽  
Test Method ◽  
Prick Test ◽  
Ige Mediated

scholarly journals Skin Testing to Identify Safe Drugs for Patients with Rocuronium-Induced Anaphylaxis

2020 ◽  
Vol 2020 ◽  
pp. 1-3
Author(s):  
Manzo Suzuki ◽  
Hajime Kawase ◽  
Azusa Ogita ◽  
Hiroyasu Bito
Keyword(s):  
General Anesthesia ◽  
Skin Testing ◽  
Blocking Agent ◽  
Cross Reactivity ◽  
Neuromuscular Blocking ◽  
Intradermal Test ◽  
Skin Tests ◽  
Prick Test ◽  
Causative Drug ◽  
Ige Mediated

Among patients who develop anaphylaxis during anesthesia, anaphylaxis caused by a neuromuscular blocking agent has the highest incidence. In patients who developed IgE-mediated anaphylaxis, and cross-reactivity among NMBAs is a concern in subsequent anesthetic procedures. We present a patient who developed rocuronium-induced anaphylaxis in whom the skin prick test (SPT) and intradermal test (IDT) could identify a safe drug to use in the subsequent anesthetic procedure. A 32-year-old female developed anaphylactic shock at the induction of general anesthesia. She recovered by administration of hydrocortisone and epinephrine. Skin tests including the SPT followed by the IDT revealed rocuronium as the drug that caused anaphylaxis and vecuronium as a safe drug to use for the subsequent general anesthesia. She safely underwent surgery with general anesthesia using vecuronium one month after the skin testing. There are not many reports on the effectiveness of the SPT followed by IDT in identifying the causative drug as well as a safe drug to use in the subsequent anesthetic procedure following anaphylaxis during anesthesia. The usefulness of the SPT should be re-evaluated.

scholarly journals Comparative analysis of skin prick test with immunoblotting of different manufacturers in the context of determining the sensitization to birch allergen in patients with respiratory allergic diseases

2020 ◽  
pp. 20-27
Author(s):  
Artemii Bogomolov
Keyword(s):  
Allergic Rhinitis ◽  
Systematic Error ◽  
Skin Testing ◽  
Allergic Diseases ◽  
Specific Ige ◽  
Test Method ◽  
Atopic Asthma ◽  
Western Blots

The aim of the study was to evaluate the diagnostic parameters of various methods for determining birch allergen sensitization in patients with respiratory allergic diseases – allergic rhinitis and bronchial asthma. Materials and methods: During the study, 88 patients with allergic rhinitis and/or atopic asthma were examined with three different methods of specific allergic diagnosis (in vivo and in vitro) according to the recommendations of the ethics committee of the Pirogov Vinnitsa National Medical University, all of which were out of acute. Inclusion criteria were a diagnosis of allergic rhinitis (both intermittent and persistent) and/or atopic asthma. The pre-test was conducted according to the classic test method according to the normative documents with commercial allergen extracts. Western blots for the determination of IgE levels were performed using RIDA AllergyScreen test systems (R-Biopharm AG, Darmstadt, Germany) and Euroline (Euroimmun). Results and discussion: Systematic error of measurement results of immunoblot of different manufacturers is equal to 2.27 ku/l, which indicates the presence of a systematic difference. The distribution graph corresponds to the type of absolute systematic error graphs. The standard deviation of the difference was 11.28, which is significantly compared to the values themselves. Thus, the results of the two systems for the determination of specific IgE for birch allergen by the Rida AllergyScreen and Euroline methods are not well matched due to systematic divergence of indicators. There is a good agreement between the results of skin testing with birch allergens and the detection of specific IgE by the Rida AllergyScreen test, and between the results of skin testing with birch allergen and the detection of specific IgE by the Euroline method, there is a moderate agreement between the results.

scholarly journals Comparison of diagnostic parameters of methods for determination of sensitization to allergen D. pteronyssinus in patients with respiratory allergic diseases

2020 ◽  
pp. 19-26
Author(s):  
Artemy Ye. Bogomolov ◽  
Lyudmila I. Dubchak
Keyword(s):  
Allergic Rhinitis ◽  
Skin Testing ◽  
Allergic Diseases ◽  
Specific Ige ◽  
Test Method ◽  
Atopic Asthma ◽  
Diagnostic Significance ◽  
Western Blots ◽  
Diagnostic Parameters

The aim of the study was to evaluate the diagnostic parameters of various methods for determining allergy sensitization to the dust mite allergen Dermatophagoides pteronyssinus in patients with respiratory allergic diseases – allergic rhinitis and bronchial asthma. Materials and methods: During the study, 88 patients with allergic rhinitis and / or atopic asthma were examined with three different methods of specific allergic diagnosis (in vivo and in vitro) according to the recommendations of the ethics committee of the Pirogov Vinnitsa National Medical University, all of which were out of acute period. Inclusion criteria were a diagnosis of allergic rhinitis (both intermittent and persistent) and / or atopic asthma. The prick-test was conducted according to the classic test method by normative documents with commercial allergen extracts. Western blots for the determination of IgE levels were performed using RIDA AllergyScreen test systems (R-Biopharm AG, Darmstadt, Germany) and Euroline (Euroimmun). Results and discussion: The results of two systems for the determination of specific IgE to D. Pteronyssinus by the Rida AllergyScreen and Euroline methods have a systematic difference of values (-1.27 kU / l). There is a good agreement between the results of skin testing with D. Pteronyssinus allergens and the detection of specific IgE by the Rida AllergyScreen method, and there is a satisfactory agreement between the results of skin testing with D. Pteronyssinus allergens and detection of specific IgE by the Euroline method. According to the results of the curve of detection of specific IgE by AllergyScreen method for determination of sensitization to allergen D. Pteronyssinus has excellent diagnostic significance (AUC=0,953), detection of specific IgE by Euroline method for determination of sensitization to allergen D. Pteronyssinus – good AUC=0,7.

scholarly journals Clinical profile and skin prick test analysis in children with allergic rhinitis of North Kerala, India

2018 ◽  
Vol 5 (2) ◽  
pp. 372
Author(s):  
Shyna K. P. ◽  
Veena Kumari M. ◽  
Divya Krishnan K. ◽  
Abdul Azeez V. K.
Keyword(s):  
Allergic Rhinitis ◽  
Skin Prick Test ◽  
House Dust ◽  
House Dust Mite ◽  
Cost Effective ◽  
Allergic Diseases ◽  
Clinical Profile ◽  
Prick Test ◽  
Dust Mite ◽  
Ige Mediated

Background: Allergic rhinitis (AR) is increasing in prevalence among children globally and in India. Allergens are factors which trigger allergic rhinitis. Skin prick test (SPT) is a rapid, sensitive and cost effective test to detect IgE- mediated allergic diseases. Identification of common aeroallergens in an area is necessary, in order to educate the patient on what allergens to avoid and also help find the best formulation of allergen immunotherapy for effective AR treatment. This study was done to find the clinical profile and skin sensitivity to common allergens by skin prick test in children with allergic rhinitis between six and fifteen years.Methods: This cross-sectional study was conducted for a period of one year from January 2016 to December 2016. All children between 6 to 15 years with allergic rhinitis were included in the study. Skin prick test was done with seven most common allergens.Results: A total of 60 children with allergic rhinitis were studied and 42 patients (70%) tested positive for SPT. The major symptoms were persistent sneezing (68%), nasal itching (33%), rhinorrhea (85%) and nasal congestion (42%). The proportion of sneezers-runners was higher than blockers (64% versus 36). We tested seven common allergens and house dust mite allergen yielded the highest number of positive responses (33%) followed by cockroach (25%), alternaria (16.66%), parthenium (10%), cat dander (8.35%), sorghum (5%) and dog dander (5%). Among patients with SPT positivity; eight were positive to one allergen, thirteen were positive to two allergens, sixteen to three allergens and five to four allergens.Conclusions: Allergic Rhinitis with a number of allergic co morbidities has a significant impact on the quality of life and scholastic performance in children. Skin prick test which is a standardized, most rapid, sensitive and cost-effective test to detect IgE-mediated allergic diseases is helpful in identifying the common allergens. House dust mite is the commonest allergen tested positive in our study in children. 

scholarly journals Low Potency of Indian Dust Mite Allergen Skin Prick Test Extracts Compared to FDA-Approved Extracts: A Double-Blinded Randomized Control Trial

2018 ◽  
Vol 9 ◽  
pp. 215265671879674 ◽  
Author(s):  
Devasahayam J. Christopher ◽  
Narmada Ashok ◽  
Aruna Ravivarma ◽  
Deepa Shankar ◽  
Erik Peterson ◽  
...  
Keyword(s):  
Immunoglobulin E ◽  
Skin Prick Testing ◽  
Statistical Significance ◽  
Skin Testing ◽  
Allergic Diseases ◽  
Allergen Extract ◽  
Prick Test ◽  
Full Strength ◽  
Dust Mite ◽  
Wheal Size

Background Skin prick testing is the most important diagnostic tool to detect immunoglobulin E-mediated allergic diseases. With increase in the number of allergy tests performed in India, it is imperative to know the potency of indigenous extracts in comparison with U.S. Food and Drug Administration (USFDA)-approved extracts. Methods A randomized comparison trial of Indian manufactured and USFDA-approved extracts of Dermatophagoides pteronyssinus (DP) and Dermatophagoides farinae (DF) was done at Christian Medical College & Hospital, Vellore, India from April 2014 to June 2015, to compare the skin test reactivity of indigenous allergen extracts of dust mites against validated allergen. Study enrollment included 197 patients with allergic disorders that showed sensitivity to dust mite during routine allergy skin testing. Study participants were tested with varying dilutions of DP and DF indigenous extracts along with USFDA-approved allergens in a blinded fashion. Results were recorded, and statistical significance was calculated using the Friedman rank sum test. Results Using the Friedman rank sum test with a Tukey adjustment for multiple comparisons, we found that the extracts in each dilution were significantly different ( P < .0001). The full strength indigenous extracts, B-DF (DF allergen standard extract from Bioproducts and Diagnostics, India) and C-DF (DF allergen extract from Creative Diagnostics, India) extracts, had mean wheal sizes of 7.69 (standard deviation [SD] 9.91) and 31.01(SD 51.04), respectively. The full strength S-DF (DF allergen extract from Jubilant Hollister Stier, Spokane, WA, USA) had a mean wheal size of 109.97 (SD 162.73), which was significantly higher ( P < .0001) than both the indigenous extracts. For each of the dilutions, the S-DF mean wheal size was significantly greater than that of the corresponding B-DF and C-DF wheal sizes. The full strength indigenous C-DP (DP allergen extract from Creative Diagnostics, India) had mean wheal size of 39.37 (SD 51.74). The full strength standard S-DP (DP allergen extract from Jubilant Hollister Stier, Spokane, WA, USA) extract had a mean wheal size of 167.66 (SD 270.80), which was significantly higher ( P < .0001) than the indigenous C-DP extract. Similar differences were seen across all dilutions. Conclusion The indigenous extracts have significantly lower potency compared to USFDA-approved extracts; hence, there is an urgent need for policy makers to institute stringent criteria for standardization of antigens in India.

Immunotheraphy in Allergic Diseases

2018 ◽  
Vol 24 (11) ◽  
pp. 1174-1194
Author(s):  
Albert Roger ◽  
Maria Basagana ◽  
Aina Teniente-Serra ◽  
Nathalie Depreux ◽  
Yanina Jurgens ◽  
...  
Keyword(s):  
Specific Immunotherapy ◽  
Allergic Diseases ◽  
Specific Ige ◽  
World Population ◽  
Allergen Specific Immunotherapy ◽  
Routes Of Administration ◽  
Disease Modifying Therapy ◽  
History Of ◽  
Ige Mediated ◽  
Special Case

The prevalence of allergic diseases is increasing worldwide. It is estimated that more than 30% of the world population is now affected by one or more allergic conditions and a high proportion of this increase is in young people. The diagnosis of allergy is dependent on a history of symptoms on exposure to an allergen together with the detection of allergen-specific IgE. Accurate diagnosis of allergies opens up therapeutic options. Allergen specific immunotherapy is the only successful disease-modifying therapy for IgE-mediated allergic diseases. New therapeutic strategies have been developed or are currently under clinical trials. Besides new routes of administration, new types of allergens are being developed. The use of adjuvants may amplify the immune response towards tolerance to the antigens. In this review, we analyze different antigen-specific immunotherapies according to administration route, type of antigens and adjuvants, and we address the special case of food allergy.

scholarly journals Immunologic Pathophysiology and Airway Remodeling Mechanism in Severe Asthma: Focused on IgE-Mediated Pathways

Diagnostics ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 83
Author(s):  
Shih-Lung Cheng
Keyword(s):  
Severe Asthma ◽  
Immunoglobulin E ◽  
Airway Remodeling ◽  
Real Life ◽  
Allergic Diseases ◽  
Proinflammatory Mediators ◽  
Small Subgroup ◽  
Humanized Monoclonal Antibody ◽  
Severe Allergic Asthma ◽  
Ige Mediated

Despite the expansion of the understanding in asthma pathophysiology and the continual advances in disease management, a small subgroup of patients remains partially controlled or refractory to standard treatments. Upon the identification of immunoglobulin E (IgE) and other inflammatory mediators, investigations and developments of targeted agents have thrived. Omalizumab is a humanized monoclonal antibody that specifically targets the circulating IgE, which in turn impedes and reduces subsequent releases of the proinflammatory mediators. In the past decade, omalizumab has been proven to be efficacious and well-tolerated in the treatment of moderate-to-severe asthma in both trials and real-life studies, most notably in reducing exacerbation rates and corticosteroid use. While growing evidence has demonstrated that omalizumab may be potentially beneficial in treating other allergic diseases, its indication remains confined to treating severe allergic asthma and chronic idiopathic urticaria. Future efforts may be bestowed on determining the optimal length of omalizumab treatment, seeking biomarkers that could better predict treatment response and as well as extending its indications.

scholarly journals 162. Assessment of Beta-lactam Allergies as Rationale for Receipt of Vancomycin for Surgical Prophylaxis

2020 ◽  
Vol 7 (Supplement_1) ◽  
pp. S90-S90
Author(s):  
Kendall J Tucker ◽  
YoungYoon Ham ◽  
Haley K Holmer ◽  
Caitlin M McCracken ◽  
Ellie Sukerman ◽  
...  
Keyword(s):  
Antimicrobial Prophylaxis ◽  
Skin Testing ◽  
Cross Reactivity ◽  
Penicillin Allergy ◽  
Beta Lactam ◽  
First Line ◽  
Surgical Prophylaxis ◽  
Inappropriate Use ◽  
Oral Amoxicillin ◽  
Ige Mediated

Abstract Background Beta-lactam (BL) antibiotics are first-line agents for most patients receiving antimicrobial prophylaxis in surgical procedures. Despite evidence showing low cross-reactivity between classes of BLs, patients with allergies commonly receive vancomycin as an alternative to avoid allergic reaction. The objective of this study was to identify potentially inappropriate use of vancomycin surgical prophylaxis among patients with reported BL allergies. Methods Adult patients (≥18 years) receiving vancomycin for surgical prophylaxis with a reported penicillin and/or cephalosporin allergy at our institution between August 2017 to July 2018 were retrospectively evaluated for potential eligibility for penicillin allergy testing and/or receipt of standard prophylaxis. Surgery type and allergy history were extracted from the electronic medical record. Per our institution’s penicillin-testing protocol, patients with IgE-mediated reactions &lt; 10 years ago were eligible for penicillin skin testing (PST), mild reactions or IgE-mediated reaction &gt; 10 years ago were eligible for direct oral amoxicillin challenge, and severe non-IgE mediated allergies were ineligible for penicillin allergy evaluation or BL prophylaxis. Results Among 830 patients who received vancomycin for surgical prophylaxis, 196 reported BL allergy and were included in the analysis (155 with penicillin allergy alone; 21 with cephalosporin allergy; 20 with both cephalosporin and penicillin allergy). Approximately 40% of surgeries were orthopedic. Six patients were ineligible for BL prophylaxis. Per institutional protocol, 73 of 155 patients (48%) may have qualified for PST; 81 of 155 (52%) patients may have received a direct oral amoxicillin challenge. Only 3 of 22 patients with history of methicillin-resistant Staphylococcus aureus appropriately received additional prophylaxis with vancomycin and a BL. Conclusion Patients with BL allergies often qualify for receipt of a first-line BL antibiotic. An opportunity exists for improved BL allergy assessment as an antimicrobial stewardship intervention. Future studies should evaluate outcomes associated with BL allergy evaluation and delabeling in patients receiving surgical prophylaxis. Disclosures All Authors: No reported disclosures

scholarly journals Allergen-specific immunotherapy. Analytic review of current international and Russian federal position papers

2017 ◽  
Vol 14 (1) ◽  
pp. 24-32
Author(s):  
O M Kurbacheva ◽  
K S Pavlova ◽  
M A Galitzkaya
Keyword(s):  
Specific Immunotherapy ◽  
Allergic Diseases ◽  
Allergen Specific Immunotherapy ◽  
Detailed Understanding ◽  
Analytic Review ◽  
Ige Mediated ◽  
Federal Guidelines

The article analyses the international position papers devoted to allergen-specific immunotherapy published in recent years. It also describes in details Russian federal guidelines, published in the 2013-2016 years and dedicated to allergen-specific immunotherapy as a method of treatment of IgE-mediated allergic diseases, as well as Russian federal guidelines. Detailed understanding of indications, contraindications, and usage of AIT in various clinical situations are widely discussed.

scholarly journals Mosquito hypersensitivity may be associated with atopic background in children

2021 ◽  
Vol 49 (6) ◽  
pp. 67-72
Author(s):  
Süleyman Tolga Yavuz ◽  
Onur Akin ◽  
Ozan Koc ◽  
Ali Gungor ◽  
Ahmet Bolat ◽  
...  
Keyword(s):  
Grass Pollen ◽  
Matched Control ◽  
Allergic Diseases ◽  
Atopic Disease ◽  
Duration Of Symptoms ◽  
Prick Test ◽  
Pediatric Allergy ◽  
Systemic Reactions ◽  
Dust Mite ◽  
Children With Asthma

Background: Many children encounter unusual or “exaggerated” reactions such as large local, atypical or systemic reactions after mosquito bites.Objective: The aim of this study was to document the clinical features of children with mos-quito allergy and investigate the possible associations between demographic features and type of reactions in this population.Methods: Children with large local or unusual reactions after mosquito bites who attended to our outpatient pediatric allergy department were enrolled in the study along with control subjects.Results: A total of 180 children (94 with mosquito allergy and 86 age and sex-matched control subjects) with a median age of 6.8 years (IQR 5.5–9.3) were enrolled. Atopy (35.1% vs. 11.6%, p < 0.001) and grass pollen sensitization (28.7% vs. 8.1%, p < 0.001) were significantly more frequent in children with mosquito allergy. Skin prick test with mosquito allergen was positive in only 6 children (6,4%). Grass pollen sensitization was most common in children (28.7%) followed by sensitization to house dust mite (9.6%). 30 children (31.9%) had an accompanying atopic disease such as allergic rhinitis, asthma or atopic dermatitis. Bullae were significantly more frequent in children with asthma (41.7% vs.15.9, p = 0.034). The median duration of symptoms after onset were significantly longer in patients with ecchymosis, with immediate wheals and in children whose symptoms start in 20 min to 4 hours after mosquito bites.Conclusion: There is an association between unusual, large local or exaggerated reactions after mosquito bites and allergic diseases in children. The severity of reactions increases with age and particularly in children with atopic background.

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