Penicillin V Benzathine

2021 ◽  
Keyword(s):  
Penicillin V

scholarly journals Enzyme-catalyzed biodegradation of penicillin fermentation residues by β-lactamase OtLac from Ochrobactrum tritici

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Peng Wang ◽  
Chen Shen ◽  
Qinqin Cong ◽  
Kaili Xu ◽  
Jialin Lu
Keyword(s):  
Large Scale ◽  
In Vitro Study ◽  
Scale Removal ◽  
E Coli ◽  
Penicillin V ◽  
Highly Active ◽  
Steady State Kinetics ◽  
Biodegradation Process ◽  
Km Value

Abstract Background Biodegradation of antibiotics is a promising method for the large-scale removal of antibiotic residues in the environment. However, the enzyme that is involved in the biodegradation process is the key information to be revealed. Results In this study, the beta-lactamase from Ochrobactrumtritici that mediates the biodegradation of penicillin V was identified and characterized. When searching the proteins of Ochrobactrumtritici, the β-lactamase (OtLac) was identified. OtLac consists of 347 amino acids, and predicted isoelectric point is 7.0. It is a class C β-lactamase according to BLAST analysis. The coding gene of OtLac was amplified from the genomic DNA of Ochrobactrumtritici. The OtLac was overexpressed in E. coli BL21 (DE3) and purified with Ni2+ column affinity chromatography. The biodegradation ability of penicillin V by OtLac was identified in an in vitro study and analyzed by HPLC. The optimal temperature for OtLac is 32 ℃ and the optimal pH is 7.0. Steady-state kinetics showed that OtLac was highly active against penicillin V with a Km value of 17.86 μM and a kcat value of 25.28 s−1 respectively. Conclusions OtLac demonstrated biodegradation activity towards penicillin V potassium, indicating that OtLac is expected to degrade penicillin V in the future.

Havapen et al

1967 ◽  
Vol 5 (7) ◽  
pp. 28-28 ◽  
Keyword(s):  
Oral Dose ◽  
Gastric Acid ◽  
Plasma Protein ◽  
Penicillin G ◽  
Controlled Trials ◽  
Penicillin V ◽  
Acid Stable

Gastric acid partially destroys benzylpenicillin (penicillin G) and only a small and inconsistent fraction of an oral dose is absorbed. To be certain of its effect the drug must be injected. A number of acid-stable penicillins with the antibacterial range of benzylpenicillin but quite well absorbed from the gut are now available, phenoxymethylpenicillin (penicillin V) for example. In vitro phenoxymethylpenicillin is a little less effective than benzylpenicillin against streptococci1 and considerably less effective against H. influenzae and gonococci. In addition, a higher proportion of phenoxymethylpenicillin than of benzylpenicillin is bound to plasma protein.2 Though controlled trials show that oral benzylpenicillin is as effective as oral phenoxymethylpencillin for most patients with susceptible infections treated outside hospital,3–6 our consultants prefer phenoxymethylpenicillin because absorption is less variable.

Antibiotic susceptibility pattern against pathogenic bacteria causing Dental Caries

2011 ◽  
Vol 1 (1) ◽  
pp. 31-35
Author(s):  
Deepak Dwivedi ◽  
Tejram Kushwah ◽  
Mukesh Kushwah ◽  
Vinod Singh
Keyword(s):  
Dental Caries ◽  
Antibiotic Susceptibility ◽  
Pathogenic Bacteria ◽  
Resistant Bacteria ◽  
Susceptibility Pattern ◽  
Antibiotic Resistant ◽  
Antibiotic Susceptibility Pattern ◽  
Penicillin V ◽  
Antibiotics Susceptibility ◽  
Susceptibility Tests

Antibiotics to treat dental caries infection are routinely prescribed which led to the increased resistance against bacteria. The purpose of this investigation was to perform antibiotic susceptibility tests on a panel of pathogenic bacteria isolated from dental caries infection. Bacteria were isolated from caries site of patients and identified at the species level. Each of 150 species of bacteria was tested for antibiotics susceptibility to a five antibiotics using Etest. The antibiotics used were Amoxicillin, Cloxocillin, Erythromycin, Tetracycline and Penicillin‐V. The obtained resistance percentage for each antibiotic were Penicillin V: 72/150 (48%), Tetracycline: 99/150 (66%), Amoxicillin: 135/150 (90%), Cloxocillin: 117/150 (78%), and Erythromycin: 90/150 (60%) (Table 1). In case of combinatorial antibiotic exposure, the resistance percentage of Penicillin V/Amoxicillin and Amoxicillin/ Erythromycin was 39/150 (26%), and 45/150 (30%) respectively. The study has well demonstrated the clinical picture of antibiotic resistance and susceptibility pattern of bacteria causing dental caries. The obtained comprehensive data will allow investigating the spatial distribution of pathogenic, antibiotic resistant bacteria among dental caries patients which further may help into development of novel diagnostic and treatment approaches for the same.

The use of a Mercury-Penicillin V Derivative to Localize Penicillin-Binding Proteins in Escherichia coli

1993 ◽  
pp. 295-301
Author(s):  
Terry R. Paul ◽  
Terry J. Beveridge ◽  
Noreen G. Halligan ◽  
Larry C. Blaszczak ◽  
Tom R. Parr
Keyword(s):  
Escherichia Coli ◽  
Binding Proteins ◽  
Penicillin Binding Proteins ◽  
Penicillin V
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