scholarly journals CYTOGENETIC CONSEQUENCES OF THE MIXED EFFECTS OF OPISTORCHIASIS AND TICK-BORNED ENCEPHALITIS IN ASSOCIATION WITH POLYMORPHISM BY GLUTHATHIONE-S-TRANSFERASE GENES

2021 ◽  
pp. 209-214
Author(s):  
N. Ilyinskikh ◽  
E. Ilyinskikh ◽  
Filatova
Keyword(s):  
Multiplex Pcr ◽  
Mixed Effects ◽  
Glutathione S Transferase ◽  
Opisthorchis Felineus ◽  
Cytogenetic Effects ◽  
Tick Borne Encephalitis ◽  
Cytogenetic Changes ◽  
Tickborne Encephalitis

Purpose: to study the duration of the preservation of the cytogenetic effects of tickborne encephalitis (TBE) in individuals infected with the Opisthorchis felineus (OF) helminth, which differ in alleles of the glutathione-S-transferase enzyme genes. Methods: the objects of the study were patients with tick-borne encephalitis in whom the frequencies of micronucleated lymphocytes were detected. All examined patients were subdivided into two groups: TBE patients non-infected with OF, and TBE patients infected with OF. Multiplex PCR was used to analyze the GSTM1 and GSTT1 genes. Results: patients with TBE, infected with OF, had a significantly lower increase in the level of cytogenetic disorders than patients with TBE without OF infection; at the same time, the restoration of the cytogenetic norm in this group was not reached, and there was no association between the frequency of micronucleated lymphocytes and different variants of the glutathione-S-transferase enzyme genes. Conclusion: the frequency of cytogenetic changes in TBE patients infected with OF are significantly less than one in patients with TBE without OF infection. Polymorphism in the genes of the enzyme glutathione-S-transferase does not have significant effect on the cytogenetic disorders in patients with TBE with OF infection.

Monitoring the cytogenetic consequences of tick-borne encephalitis occurring with opisthorchiasis invasion due to glutathione-S-transferase gene polymorphisms GSTM1 and GSTTA1

2020 ◽  
Vol 1_2020 ◽  
pp. 75-79
Author(s):  
Ilyinskikh N.N. Ilyinskikh ◽  
Ilyinskikh E.N. Ilyinskikh ◽  
Kostromeeva M.V. Kostromeeva ◽  
Filatova E.N. Filatova E ◽  
◽  
...  
Keyword(s):  
Gene Polymorphisms ◽  
Glutathione S Transferase ◽  
Transferase Gene ◽  
Tick Borne Encephalitis

scholarly journals Monitoring of cytogenetic instability by micronuclei assay of both immunocompetent and non-immunocompetent cells in tick-borne encephalitis patients depending on variants of glutathione-S-transferase genes in the genotype

2019 ◽  
Vol 9 (3-4) ◽  
pp. 600-606
Author(s):  
Nikolay Nikolaevich Ilyinskikh ◽  
Ekaterina Nikolaevna Ilyinskikh ◽  
Evgenia Vladimirovna Zamyatina ◽  
Svetoslava Vyacheslavovna Lee
Keyword(s):  
T Lymphocytes ◽  
Peripheral Blood ◽  
Micronucleus Assay ◽  
Immunocompetent Cells ◽  
Control Group ◽  
Buccal Cells ◽  
Glutathione S Transferase ◽  
Tick Borne Encephalitis ◽  
Buccal Mucous Membrane ◽  
The One

Aim of this study was to study the dynamics of the frequency of cytokinesis-blocked T-lymphocytes with micronuclei in peripheral blood and the frequency of buccal micronucleated epithelium cells for a period of half a year in patients with acute tick-borne encephalitis, depending on burden of active and inactive variants of glutathione-S-transferase genes (GSTM1 and GSTT1) in the patient's genotype. We carried out micronucleus assay in immunocompetent and non-immunocompetent cells in 54 patients with acute tick-borne encephalitis and 35 healthy persons (control) residing in the Tomsk and Tyumen regions. To analyze the frequency of cytokinesis-blocked micronucleated T-lymphocytes was used venous peripheral blood as material for phytohemagglutinin-stimulated cultures, and to study the frequency of buccal micronucleated cells, samples of the buccal mucous membrane epithelial cells were obtained. To carried out both techniques of micronucleus assay, cytological preparations were prepared, which were stained using the Giemsa or Felgen methods. The material for the study was obtained repeatedly during admission of patients to treatment, and also after 1 week, 1, 3 and 6 months.  Polymerase chain reaction was used to analyze the alleles of the GSTM1 and GSTT1 genes. As a result of this analysis was found a significant increase in the frequency of micronucleated cells in tick-borne encephalitis patients compared with the control group. In addition, the frequency of cytokinesis-blocked micronucleated T-lymphocytes was increased significantly higher than the one of micronucleated buccal cells. The most significant and prolonged increase in the frequency of micronucleated cells was associated with the mutant inactive variants of the genes GSTM1 (0/0) and GSTT1 (0/0). In the patients with burden the inactive forms of these genes, the cytogenetic instability of the cytokinesis-blocked blood T-lymphocytes could persist for up to six months. In case of buccal cells, the frequency of micronucleated cells was close to the one in the control group as early as 1-3 months after a course of treatment. Conclusion. It was found that the most increased and prolonged frequency of cytogenetically instable cells persisted in cytokinesis-blocked T-lymphocytes of peripheral blood of patients with tick-borne encephalitis who were carriers of the genotype with inactive variants of  both GSTM1 (0/0) and GSTT1 (0/0 ) glutathione-S-transferase genes.

scholarly journals Polymorphisms of the GSTT1 and GSTM1 genes in women of central Serbia: Absence of association with uterine myoma

2013 ◽  
Vol 65 (2) ◽  
pp. 415-420 ◽  
Author(s):  
S. Arsenijevic ◽  
Biljana Ljujic ◽  
Ivana Stosic ◽  
D. Grujicic ◽  
D. Marinkovic ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Significant Risk ◽  
Uterine Myoma ◽  
Glutathione S Transferase ◽  
Cellular Protection ◽  
Healthy Control ◽  
Gst Polymorphism

Since glutathione S-transferase (GST) enzymes are involved in cellular protection, we aimed to determine the distribution of GSTT1 and GSTM1 null genotypes in women in central Serbia in order to assess the risk of development of uterine myoma. The study consisted of 34 clinically diagnosed uterine myoma patients and 35 healthy control women. Analyses of GST polymorphism were carried out by multiplex PCR. Our results showed no significant differences in the GSTT1 and GSTM1 null genotypes between the patients and controls. Using the GSTT1 positive/GSTM1 positive combination as reference, there was no statistically significant risk of uterine myoma with the combination of GSTT1 null and GSTM1 null genotypes. We conclude that polymorphism of both GSTT1 and GSTM1 genes, alone or in combination, did not present the main risk for uterine myoma in women from central Serbia.

Analysis of the Glutathione S-transferase M1 gene using pyrosequencing and multiplex PCR–no evidence of association to glaucoma

2003 ◽  
Vol 77 (2) ◽  
pp. 239-243 ◽  
Author(s):  
Mattias Jansson ◽  
Alvaro Rada ◽  
Lidija Tomic ◽  
Lill-Inger Larsson ◽  
Claes Wadelius
Keyword(s):  
Multiplex Pcr ◽  
Glutathione S Transferase

scholarly journals Monitoring of cytogenetic effects in tick-borne encephalitis patients with type 2 diabetes mellitus, depending on polymorphism of glutathione-S-transferase genes

2019 ◽  
Vol 22 (3) ◽  
pp. 225-232
Author(s):  
Nikolay N. Ilyinskikh ◽  
Marina S. Kostromeeva ◽  
Ekaterina N. Ilyinskikh
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Clinical Symptoms ◽  
Micronucleus Assay ◽  
Febrile Illness ◽  
Buccal Cells ◽  
Glutathione S Transferase ◽  
Tick Borne Encephalitis

BACKGROUND: Tick-borne encephalitis (TBE) is an acute viral disease with activation of oxidative stress and increasing in cytogenetic instability. Clinical symptoms of infectious diseases usually more severe in patients with type 2 diabetes mellitus (DM2), especially in the case of burden in the genotype of mutant variants of glutathione-S-transferase genes GSTM1 and GSTT1. AIMS: The aim of this study was to study the dynamics of the frequency of micronucleated cells in patients with acute TBE with concomitant DM2, depending on the burden of active and inactive variants of glutathione-S-transferase genes (GSTM1 and GSTT1) in the patients genotype. MATERIALS AND METHODS: Totally, samples to make micronucleus assay were obtained from 138 patients with febrile illness of acute TBE, 64 of whom were diagnosed with concomitant DM2 (groups 3 and 4). As control groups, 57 healthy individuals (control 1) and 61 patients with DM2 (control 2) were examined. The samples of buccal cells for the micronucleus assay were repeatedly obtained from the individuals on the first day of admission, and also after 1 week, 1, 3 and 6 months. Polymerase chain reaction was used to analyze the variants of the GSTM1 and GSTT1 genes. RESULTS: On the first days of the disease, significant increases in the frequency of micronucleated buccal cells were determined in all TBE patients as compared to controls 1 and 2 (P0.001). Significant increases in the frequency of micronucleated buccal cells was revealed in groups 3 and 4 of the TBE patients who were carriers of inactive variants of the GSTM1(0) and GSTT1(0) genes, as compared to the subgroup of TBE patients with active variants of these genes (P0.001). In all subgroups of TBE patients with concomitant DM2, the frequencies of micronucleated cells were significantly higher than in the subgroups of TBE patients without DM2 (P0.001). Study of the dynamics of the frequency of micronucleated buccal cells, as compared to the control, demonstrated that the highest and long-lasting (within 6 months) cytogenetical effects were maintained in the group of TBE patients with genotype GSTM1(0)/GSTT1 (0) and concomitant DM2. CONCLUSION: The most prolonged and highest increases in the frequencies of cytogenetically instable cells were determined in the group of acute TBE patients with concomitant DM2 who were carriers of the genotype with inactive variants of both GSTM1(0) and GSTT1(0) glutathione-S-transferase genes.

Multiplex PCR–single-base extension genotyping of multiple glutathione S-transferase polymorphisms

2005 ◽  
Vol 41 (1) ◽  
pp. 9 ◽  
Author(s):  
Guilherme N. M. Ferreira ◽  
Ana Faber ◽  
Susana N. Silva ◽  
José Rueff ◽  
Jorge Gaspar
Keyword(s):  
Multiplex Pcr ◽  
Single Base Extension ◽  
Glutathione S Transferase ◽  
Single Base ◽  
Base Extension
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