Polymorphisms of the GSTT1 and GSTM1 genes in women of central Serbia: Absence of association with uterine myoma

Since glutathione S-transferase (GST) enzymes are involved in cellular protection, we aimed to determine the distribution of GSTT1 and GSTM1 null genotypes in women in central Serbia in order to assess the risk of development of uterine myoma. The study consisted of 34 clinically diagnosed uterine myoma patients and 35 healthy control women. Analyses of GST polymorphism were carried out by multiplex PCR. Our results showed no significant differences in the GSTT1 and GSTM1 null genotypes between the patients and controls. Using the GSTT1 positive/GSTM1 positive combination as reference, there was no statistically significant risk of uterine myoma with the combination of GSTT1 null and GSTM1 null genotypes. We conclude that polymorphism of both GSTT1 and GSTM1 genes, alone or in combination, did not present the main risk for uterine myoma in women from central Serbia.

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Analysis of glutathione S-transferase genes polymorphisms and the risk of schizophrenia in a sample of Iranian population

Neuron Glia Biology ◽

10.1017/s1740925x12000130 ◽

2011 ◽

Vol 7 (2-4) ◽

pp. 199-203 ◽

Cited By ~ 7

Author(s):

Farah Lotfi Kashani ◽

Dor Mohammad Kordi-Tamandani ◽

Roya Sahranavard ◽

Mohammad Hashemi ◽

Farzaneh Kordi-Tamandani ◽

...

Keyword(s):

Genomic Dna ◽

Case Control Study ◽

Gene Interaction ◽

Case Control ◽

Glutathione S Transferase ◽

Chain Reaction ◽

Healthy Control ◽

Glutathione S Transferases ◽

Polymerase Chain ◽

Control Study

Glutathione S-transferases (GSTs) are major intracellular antioxidants, which, impaired in their function, are involved in the progress of schizophrenia (SCZ). The aim of this case-control study was to investigate the association between the polymorphism of glutathione S-transferases M1 (GSTM1), T1 (GSTT1), the glutathione S-transferase P1 gene (GSTP1) and SCZ. We isolated genomic DNA from peripheral blood of 93 individuals with SCZ and 99 healthy control subjects' genotypes analyzing them for GSTM1, GSTT1 and GSTP1 using polymerase chain reaction. The analysis of the gene–gene interaction between GSTs indicated that the magnitude of the association was greater for the combined AG/GSTT1 & GSTM1 genotypes (OR = 2.51; 95% CI: 1.13–5.63, P = 0.02). The AG and combined AG + GG genotypes of GSTP1 increased the risk of SCZ (OR = 1.83; 95% CI: 0.94–3.75 and OR = 1.71; 95% CI: 0.92–3.19, respectively). The genotypes of GSTT/NULL, NULL/GSTM and NULL/NULL increased the risk of SCZ (OR = 2.05; 95% CI: 0.9–4.74; OR = 2.0; 95% CI: 1.68–2.31; and OR = 1.8; 95% CI: 0.57–2.46, respectively). The present study supports previous data that suggest that impairment in the function of GSTs genes may increase the risk of SCZ.

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Association between Inflammatory Marker, Environmental Lead Exposure, and Glutathione S-Transferase Gene

BioMed Research International ◽

10.1155/2013/474963 ◽

2013 ◽

Vol 2013 ◽

pp. 1-6 ◽

Cited By ~ 34

Author(s):

Jintana Sirivarasai ◽

Winai Wananukul ◽

Sming Kaojarern ◽

Suwannee Chanprasertyothin ◽

Nisakron Thongmung ◽

...

Keyword(s):

Lead Exposure ◽

Inflammatory Marker ◽

Blood Lead ◽

Genetic Determinants ◽

Glutathione S Transferase ◽

Environmental Lead ◽

Gst Polymorphisms ◽

Gst Polymorphism ◽

Hs Crp ◽

Environmental Lead Exposure

A number of studies suggested that lead is related to the induction of oxidative stress, and alteration of immune response. In addition, modifying these toxic effects varied partly by GST polymorphism. The objectives of this study were to assess the association between the lead-induced alteration in serum hs-CRP, with GSTM1, GSTT1, and GSTP1 Val105Ile genetic variations and the health consequence from environmental lead exposure. The 924 blood samples were analyzed for blood lead, CRP, and genotyping of three genes with real-time PCR. Means of blood lead and serum hs-CRP were 5.45 μg/dL and 2.07 mg/L. Both CRP and systolic blood pressure levels were significantly higher for individuals with blood lead in quartile 4 (6.48–24.63 μg/dL) compared with those in quartile 1 (1.23–3.47 μg/dL,P<0.01). In particular, in men with blood lead >6.47 μg/dL the adjusted odds ratio (OR) of CRP levels for individuals with GSTP1 variants allele, GSTM1 null, GSTT1 null, double-null GSTM1, and GSTT1 compared with wild-type allele was 1.46 (95% CI; 1.05–2.20), 1.32 (95% CI; 1.03–1.69), 1.65 (95% CI; 1.17–2.35), and 1.98 (95% CI; 1.47–2.55), respectively. Our findings suggested that lead exposure is associated with adverse changes in inflammatory marker and SBP. GST polymorphisms are among the genetic determinants related to lead-induced inflammatory response.

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Comparison Of Total Serum Sulfhydryl and Glutathione S Transferase Activities in Patients With Oral Cavity Malignancies and Healthy Control Individuals

International Journal of Academic Medicine and Pharmacy ◽

10.29228/jamp.42882 ◽

2020 ◽

Vol Volume: 2 Issue: 2 (Volume: 2 Issue: 2) ◽

pp. 72-75

Author(s):

Şeyda Belli- Şeyda Belli, Halit Demir, Bilge Özdemir, Canan demir

Keyword(s):

Oral Cavity ◽

Total Serum ◽

Glutathione S Transferase ◽

Healthy Control

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Glutathione-S-Transferase M1 and T1 Gene Polymorphisms and Susceptibility to the Progression of Liver Fibrosis in Hcv-Infected Patients in Taiwan

Journal of Medical Biochemistry ◽

10.2478/jomb-2013-0028 ◽

2014 ◽

Vol 33 (3) ◽

pp. 259-264 ◽

Cited By ~ 1

Author(s):

Fang-Chun Sun ◽

Ya-Chung Jeng ◽

Meng-Shiou Lee ◽

Chen-Fan Wen ◽

Tsung-Ming Chen ◽

...

Keyword(s):

Liver Fibrosis ◽

Odds Ratio ◽

Gene Polymorphisms ◽

Healthy Controls ◽

Hepatitis C Virus Infection ◽

Glutathione S Transferase ◽

Significant Relation ◽

Gstt1 Gene ◽

Healthy Control ◽

T1 Gene

Abstract Background: This study was conducted to evaluate the influence of glutathione-S-transferase (GST) M1 and T1 polymorphisms in 184 patients with different stages of liver fibrosis and hepatitis C virus infection and 173 healthy control subjects. Methods: DNA samples were extracted from whole blood, and the polymorphisms of GSTM1 and GSTT1 were determined with PCR using fluorescence-labeled Taq Man probes. Associations between specific genotypes and progression of liver fibrosis were examined by use of the logistic regression analysis to calculate the odds ratio (OR) and 95% confidence intervals (CI). Results: Results show that no differences were found between the frequencies of GSTM1 (49.8% versus 50.2%) and GSTT1 (52.2% versus 47.8%) null genotypes in HCV-infected pa tients and healthy controls, respectively. In addition, there was also no significant relation between the frequency of GSTM1 or GSTT1 gene polymorphisms and fibrosis stage as classified by the METAVIR group. Conclusions: The combined GSTM1 and GSTT1 null genotypes showed an association between GSTM1 [-]/GSTT1 [- ] and progression of liver fibrosis.

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Herpesvirus type 7 infection may play an important role in individuals with a genetic profile of susceptibility to Graves' disease

Acta Endocrinologica ◽

10.1530/eje-09-0719 ◽

2010 ◽

Vol 162 (2) ◽

pp. 315-321 ◽

Cited By ~ 14

Author(s):

Janaina Luisa Leite ◽

Natassia Elena Bufalo ◽

Roberto Bernardo Santos ◽

João Hamilton Romaldini ◽

Laura Sterian Ward

Keyword(s):

Bacterial Infections ◽

Significant Risk ◽

Function Evaluation ◽

Graves Disease ◽

Genetic Profile ◽

Glutathione S Transferase ◽

Herpesvirus Type ◽

History Of ◽

Cytochrome P450 Family ◽

Design And Methods

ObjectiveAn inherited profile of genes related to the response to aggressive environmental factors such as viruses and chemicals may be related to an increased susceptibility to Graves' disease (GD).Design and methodsThis prospective case–control study was designed to examine the relationship between human herpesviruses (HHV) infection, determined by circulating DNA; tumour protein p53 (TP53) apoptotic ability; and detoxification system genes, and GD. We studied 280 confirmed GD patients paired to 284 controls with respect to environmental exposure. Exclusion criteria included medications that could interfere with thyroid function evaluation and a recent history of viral and bacterial infections.ResultsA stepwise regression analysis adjusted for age, gender, and ethnicity established the inheritance of glutathione S-transferase pi 1 (GSTP1) (odds ratio (OR)=3.423; 95% confidence interval (CI)=2.120–5.527; P<0.001) and cytochrome P450, family 1, subfamily A, polypeptide 1 (CYP1A1) variants (OR=1.649; 95% CI=1.012–2.686; P=0.0445) as significant risk factors for the disease. HHV-7 infection was much more common in GD patients (64.64%) than in controls (38.73%; χ2, P<0.0001), and it increased the risk for GD more than three times (OR=3.133; 95% CI=1.959–5.011; P<0.0001). The inheritance of less efficient Pro/Pro TP53 gene variants significantly increased the risk of GD development (OR=5.196; 95% CI=2.112–12.783; P<0.0001) and also favored HHV-7 infection (OR=2.835; 95% CI=1.100–7.310; P=0.0275). In addition, 72TP53 variants augmented the risk of GD relapse (OR=1.860; 95% CI=1.015–3.410; P=0.0446).ConclusionsWe suggest that an inherited genetic profile involving TP53 may favor HHV-7 infection and maintenance, which, in turn, may initiate and perpetuate GD autoimmune process.

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Comparative Hepatotoxicity of Aflatoxin B1 among Workers Exposed to Different Organic Dust with Emphasis on Polymorphism Role of Glutathione S-Transferase Gene

Open Access Macedonian Journal of Medical Sciences ◽

10.3889/oamjms.2016.051 ◽

2016 ◽

Vol 4 (2) ◽

pp. 312-318 ◽

Cited By ~ 7

Author(s):

Amal Saad-Hussein ◽

Eman M. Shahy ◽

Weam Shaheen ◽

Mona M. Taha ◽

Heba Mahdy-Abdallah ◽

...

Keyword(s):

Gene Polymorphism ◽

Aflatoxin B1 ◽

Liver Enzymes ◽

Dust Exposure ◽

Organic Dust ◽

Glutathione S Transferase ◽

Exposed Workers ◽

Sawmill Workers ◽

Gst Polymorphism

AIM: The study aimed to investigate effects of organic dust exposure from different sources on aflatoxin B1-albumin adducts (AFB1/Alb), and role of glutathione S-transferase (GST) gene polymorphism in hepatotoxicity of (AFB1) among exposed workers. MATERIAL AND METHODS: Liver enzymes, AFB1/Alb, and GST polymorphism were estimated in 132 wheat flour dust and 87 woods sawmill workers, and 156 controls.RESULTS: Results revealed that AFB1/Alb and liver enzymes were significantly elevated in exposed workers compared to controls, and were significantly higher in sawmill workers compared to flour workers. AFB1/Alb in flour and sawmill workers with GSTT1 and GSTM1&GSTT1 null genotypes were significantly higher than controls, and in sawmill workers with GSTM1&GSTT1 null than flour workers. Liver enzymes (ALT and AST) in sawmill workers were significantly higher than flour workers and controls in all GST polymorphism; except in GSTT1 polymorphism, where these enzymes were significantly higher in the two exposed groups than controls.CONCLUSIONS: In conclusion, organic dust exposure may cause elevation in AFB1/Alb and liver enzymes of exposed workers, and GST gene polymorphism plays an important role in susceptibility to hepatic parenchymal cell injury; except in workers with GSTT1&GSTM1 null genotype, gene susceptibility seemed to have little role and the main role was for environmental exposures.

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Bacterial peptide methionine sulphoxide reductase: co-induction with glutathione S-transferase during chemical stress conditions

Biochemical Journal ◽

10.1042/bj3600675 ◽

2001 ◽

Vol 360 (3) ◽

pp. 675-681 ◽

Cited By ~ 9

Author(s):

Antonio TAMBURRO ◽

Nerino ALLOCATI ◽

Michele MASULLI ◽

Domenico ROTILIO ◽

Carmine DI ILIO ◽

...

Keyword(s):

Polyclonal Antibodies ◽

Mrna Level ◽

Chemical Compounds ◽

Stress Conditions ◽

Detoxification Enzymes ◽

Chemical Stress ◽

Ochrobactrum Anthropi ◽

Glutathione S Transferase ◽

Cellular Protection ◽

Glutathione S Transferases

Peptide methionine sulphoxide reductase (MsrA; EC 1.8.4.6) is a ubiquitous enzyme catalysing the reduction of methionine sulphoxide to methionine in proteins, while the glutathione S-transferases (GSTs) are a major family of detoxification enzymes. A gene homologous to MsrA was identified in a chromosomal fragment from the bacterium Ochrobactrum anthropi, and this gene is located just downstream of a GST gene identified previously (OaGST) [Favaloro, Tamburro, Angelucci, De Luca, Melino, Di Ilio and Rotilio (1998) Biochem. J. 335, 573–579]. This raises the question of whether the products of these two genes may be involved in a common cellular protection function. To test this hypothesis, the hypothetical MsrA protein has been overexpressed in Escherichia coli as a functional 51kDa GST fusion protein. Following cleavage with thrombin and purification, the soluble 24kDa protein showed MsrA activity with N-acetylmethionine sulphoxide as substrate, as well as with other sulphoxide compounds. Therefore polyclonal antibodies were raised against the recombinant protein, and the modulation of MsrA in this bacterium, grown in the presence of different stimulants simulating several stress conditions, was investigated. The level of expression of MsrA was detected both by measuring the mRNA level and by immunoblotting experiments, in addition to measuring its catalytic activity. MsrA is a constitutive enzyme which is also inducible by chemical stress involving phenolic compounds such as phenol and 4-chlorophenol. Recently we reported that the GST of this bacterium, like MsrA, is only modulated by toxic chemical compounds [Favaloro, Tamburro, Trofino, Bologna, Rotilio and Heipieper (2000) Biochem. J. 346, 553–559]; therefore this is the first indication of a co-induction of the MsrA and GST enzymes during chemical stress.

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Cytoprotective Mechanisms Mediated by Polyphenols from Chilean Native Berries against Free Radical-Induced Damage on AGS Cells

Oxidative Medicine and Cellular Longevity ◽

10.1155/2017/9808520 ◽

2017 ◽

Vol 2017 ◽

pp. 1-13 ◽

Cited By ~ 12

Author(s):

Felipe Ávila ◽

Cristina Theoduloz ◽

Camilo López-Alarcón ◽

Eva Dorta ◽

Guillermo Schmeda-Hirschmann

Keyword(s):

Free Radical ◽

Radical Scavenging ◽

Free Radical Scavenging ◽

Radical Scavenger ◽

Glyoxalase I ◽

Protective Effects ◽

Glutathione S Transferase ◽

Ags Cells ◽

Cellular Protection ◽

Glutathione S Transferases

The prevalence of cytoprotective mechanisms induced by polyphenols such as activation of intracellular antioxidant responses (ICM) and direct free radical scavenging was investigated in native Chilean species of strawberries, raspberries, and currants. Human gastric epithelial cells were co- and preincubated with polyphenolic-enriched extracts (PEEs) from Chilean raspberries (Rubus geoides), strawberries (Fragaria chiloensis ssp. chiloensis f. chiloensis), and currants (Ribes magellanicum) and challenged with peroxyl and hydroxyl radicals. Cellular protection was determined in terms of cell viability, glyoxalase I and glutathione s-transferases activities, and carboxymethyl lysine (CML) and malondialdehyde levels. Our results indicate that cytoprotection induced by ICM was the prevalent mechanism for Rubus geoides and F. chiloensis. This agreed with increased levels of glyoxalase I and glutathione S-transferase activities in cells preincubated with PEEs. ORAC index indicated that F. chiloensis was the most efficient peroxyl radical scavenger. Moreover, ICM mediated by F. chiloensis was effective in protecting cells from CML accumulation in contrast to the protective effects induced by free radical scavenging. Our results indicate that although both polyphenol-mediated mechanisms can exert protective effects, ICM was the most prevalent in AGS cells. These results suggest a potential use of these native berries as functional food.

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