Genetic variants of the Crimean-Congo hemorrhagic fever virus circulating in endemic areas of Southern Tajikistan in 2009

2013 ◽  
Vol 28 (3) ◽  
pp. 119-126 ◽  
Author(s):  
I. D. Petrova ◽  
Y. V. Kononova ◽  
E. V. Chausov ◽  
A. M. Shestopalov ◽  
F. H. Tishkova
Keyword(s):  
Genetic Variants ◽  
Hemorrhagic Fever ◽  
Fever Virus ◽  
Crimean Congo Hemorrhagic Fever ◽  
Hemorrhagic Fever Virus ◽  
Endemic Areas

BSL4 Facilities in Anti-Infectious Disease Measures

2009 ◽  
Vol 4 (5) ◽  
pp. 352-355 ◽  
Author(s):  
Ichiro Kurane ◽  
Keyword(s):  
Infectious Disease ◽  
Hemorrhagic Fever ◽  
Fever Virus ◽  
Lassa Virus ◽  
South American ◽  
Environment Effect ◽  
Crimean Congo Hemorrhagic Fever ◽  
Hemorrhagic Fever Virus ◽  
Multiple Factors ◽  
Endemic Areas

Pathogens are divided into biosafety levels (BSL) 1 to 4 based on multiple factors such as virulence, transmissibility, environment effect, and treatment availability. BSL1 pathogens are the least virulent and BSL4 the most. BSL4 pathogens include ebolavirus, marburgvirus, Crimean-Congo hemorrhagic fever virus, lassa virus, variolla virus, and South American hemorrhagic fever viruses, as detailed in Table 1. Pathogens at each of the 4 BSLs must be handled in equivalently physically contained laboratories, graded P1-4. BSL4 pathogens do not exist in nature in Japan, which currently has no equivalent physical containment facilities, but the possibility exists that they may be brought into the country unintentionally by those infected in endemic areas or intentionally by bioterrorists.

scholarly journals Crimean-Congo Hemorrhagic Fever Virus (CCHFV): A Silent but Widespread Threat

2021 ◽  
Author(s):  
Paul A. Kuehnert ◽  
Christopher P. Stefan ◽  
Catherine V. Badger ◽  
Keersten M. Ricks
Keyword(s):  
One Health ◽  
Hemorrhagic Fever ◽  
Fever Virus ◽  
Virus Vector ◽  
Disease Spread ◽  
Fatality Rate ◽  
Crimean Congo Hemorrhagic Fever ◽  
Hemorrhagic Fever Virus ◽  
High Fatality Rate ◽  
Endemic Areas

Abstract Purpose of Review This review is aimed at highlighting recent research and articles on the complicated relationship between virus, vector, and host and how biosurveillance at each level informs disease spread and risk. Recent Findings While human cases of CCHFV and tick identification in non-endemic areas in 2019–2020 were reported to sites such as ProMed, there is a gap in recent published literature on these and broader CCHFV surveillance efforts from the late 2010s. Summary A review of the complex aspects of CCHFV maintenance in the environment coupled with high fatality rate and lack of vaccines and therapeutics warrants the need for a One-Health approach toward detection and increased biosurveillance programs for CCHFV.

scholarly journals Immunoglobulin-like Domain of HsFcμR as a Capture Molecule for Detection of Crimean-Congo Hemorrhagic Fever Virus- and Zika Virus-Specific IgM Antibodies

2019 ◽  
Vol 65 (3) ◽  
pp. 451-461 ◽  
Author(s):  
Anne Rackow ◽  
Christa Ehmen ◽  
Ronald von Possel ◽  
Raquel Medialdea-Carrera ◽  
David Brown ◽  
...  
Keyword(s):  
Zika Virus ◽  
Specific Binding ◽  
Hemorrhagic Fever ◽  
Fever Virus ◽  
Size Exclusion ◽  
Animal Origin ◽  
Serum Samples ◽  
Crimean Congo Hemorrhagic Fever ◽  
Hemorrhagic Fever Virus ◽  
Igm Antibodies

Abstract BACKGROUND The cellular surface molecule HsTOSO/FAIM3/HsFcμR has been identified as an IgM-specific Fc receptor expressed on lymphocytes. Here, we show that its extracellular immunoglobulin-like domain (HsFcμR-Igl) specifically binds to IgM/antigen immune complexes (ICs) and exploit this property for the development of novel detection systems for IgM antibodies directed against Crimean-Congo hemorrhagic fever virus (CCHFV) and Zika virus (ZIKV). METHODS His-tagged HsFcμR-Igl was expressed in Escherichia coli and purified by affinity chromatography, oxidative refolding, and size-exclusion chromatography. Specific binding of HsFcμR-Igl to IgM/antigen ICs was confirmed, and 2 prototypic ELISAs for the detection of anti-CCHFV and anti-ZIKV IgM antibodies were developed. Thereby, patient sera and virus-specific recombinant antigens directly labeled with horseradish peroxidase (HRP) were coincubated on HsFcμR-Igl-coated ELISA plates. Bound ICs were quantified by measuring turnover of a chromogenic HRP substrate. RESULTS Assay validation was performed using paired serum samples from 15 Kosovar patients with a PCR-confirmed CCHFV infection and 28 Brazilian patients with a PCR-confirmed ZIKV infection, along with a panel of a priori CCHFV/ZIKV-IgM-negative serum samples. Both ELISAs were highly reproducible. Sensitivity and specificity were comparable with or even exceeded in-house gold standard testing and commercial kits. Furthermore, latex beads coated with HsFcμR-Igl aggregated upon coincubation with an IgM-positive serum and HRP-labeled antigen but not with either component alone, revealing a potential for use of HsFcμR-Igl as a capture molecule in aggregation-based rapid tests. CONCLUSIONS Recombinant HsFcμR-Igl is a versatile capture molecule for IgM/antigen ICs of human and animal origin and can be applied for the development of both plate- and bead-based serological tests.

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