Inhibitory Effect of Foeniculum Vulgare Leaf Extract on Alpha-glucosidase and Alpha-amylase Activity

Objective: The present study was performed to determine in vitro antidiabetic effect of neohesperidin. To evaluate inhibitory effect of neohesperidin on α-amylase and α-glucosidase diabetes causing enzyme. Methods and Materials: Invitro carbohydrate metabolizing enzyme based inhibitory methods were used to determine antidiabetic effect of neohesperidin. Alpha (α)-amylase inhibitory assay was performed using different sources i.e. wheat alpha (α)-amylase enzyme, salivary alpha (α)-amylase and fungal alpha (α)-amylase assay. Alpha (α)-glucosidase inhibitory assay was performed using alpha (α)-glucosidase (B. stearothermophil), alpha (α)-glucosidase rat intestine and alpha (α)-glucosidase from baker’s yeast. Sucrase inhibitory assay from rat small intestine. Result: Neohesperidin possess a potent anti-diabetic by significantly inhibiting alpha amylase activity. Conclusion: It was concluded that enzyme inhibitory activity of neohesperidin shown a significantly higher inhibitory activity on alpha-amylase in comparision to alpha-glucosidase & Sucrase enzymes. Keywords: Neohespiridin, acarbose, alpha-amylase, alpha-glucosidase

Download Full-text

Enzyme-coupled ultraviolet determination of alpha-amylase activity with the GEMSAEC centrifugal analyzer.

Clinical Chemistry ◽

10.1093/clinchem/24.9.1620 ◽

1978 ◽

Vol 24 (9) ◽

pp. 1620-1624 ◽

Cited By ~ 5

Author(s):

W H Porter ◽

R E Roberts

Keyword(s):

Linear Response ◽

Amylase Activity ◽

Kinetic Studies ◽

Alpha Amylase ◽

Coefficients Of Variation ◽

Endogenous Glucose ◽

Glucose 6 Phosphate Dehydrogenase ◽

Alpha Glucosidase ◽

As Effects

Abstract We evaluated the Harleco alpha-glucosidase/hexokinase/glucose-6-phosphate dehydrogenase-coupled alpha-amylase method, bu use of the GEMSAEC centrifugal analyzer. Performance evaluation included kinetic studies of substrate and maltose hydrolysis as well as effects of endogenous glucose and fructose. The reagent was found to give a linear response with alpha-amylase activity to greater than 1200 U/liter. Within-run precision resulted in coefficients of variation (CV) of 0.9 to 3.2% over the range studied. Day-to-day precision corresponded to CV's of 2.4 to 4.4% over the same range of alpha-amylase procedure was found to be good (r = 0.997) for patients' sera examined.

Download Full-text

Inhibitory effect of 4,4′-[ethane-1,2-diylbis(sulfandiylmethanediyl)]bis(3,5-dimethyl-1H-pyrazole) and its derivatives on alpha-amylase activity

Medicinal Chemistry Research ◽

10.1007/s00044-016-1574-2 ◽

2016 ◽

Vol 25 (7) ◽

pp. 1384-1389 ◽

Cited By ~ 6

Author(s):

Vadim Maksimov ◽

Radik Zaynullin ◽

Nail Akhmadiev ◽

Elda P. Segura-Ceniceros ◽

José L. Martínez Hernández ◽

...

Keyword(s):

Amylase Activity ◽

Inhibitory Effect ◽

Alpha Amylase

Download Full-text

Inhibitory Effect of Mushroom Extracts on .ALPHA.-Amylase and .ALPHA.-Glucosidase

Nippon Shokuhin Kagaku Kogaku Kaishi ◽

10.3136/nskkk.57.532 ◽

2010 ◽

Vol 57 (12) ◽

pp. 532-538 ◽

Cited By ~ 4

Author(s):

Kazumi Ohuchi ◽

Yasuo Aoyagi

Keyword(s):

Inhibitory Effect ◽

Alpha Amylase ◽

Alpha Glucosidase ◽

Mushroom Extracts

Download Full-text

IN VITRO ANTIDIABETIC POTENTIALS OF SIDA ACUTA, ABUTILON INDICUM AND MALVASTRUM COROMANDELIANUM

International Journal of Current Pharmaceutical Research ◽

10.22159/ijcpr.2020v12i4.39088 ◽

2020 ◽

pp. 87-89

Author(s):

NANTAPORN DINLAKANONT ◽

CHANIDA PALANUVEJ ◽

NIJSIRI RUANGRUNGSI

Keyword(s):

Enzyme Inhibitors ◽

Postprandial Glucose ◽

Inhibitory Effect ◽

Glucose Absorption ◽

Alpha Amylase ◽

Sida Acuta ◽

Inhibitory Activities ◽

Alpha Glucosidase ◽

Abutilon Indicum

Objective: Starch metabolizing enzyme inhibitors are able to retard postprandial glucose absorption. This study aimed to investigate the in vitro inhibitory activities of alpha-glucosidase and alpha-amylase of three Malvaceous weeds i.e. Sidaacuta Burm. f., Abutilon indicum (Linn.) Sweet and Malvastrumcoromandelianum (Linn.) Garcke. Methods: The stems, roots and leaves of S. acuta, A. indicum and M. coromandelianum were sequentially extracted in dichloromethane and methanol, respectively. All fractions were tested for the inhibitory activities on yeast alpha-glucosidase, rat intestinal alpha-glucosidase and porcine alpha-amylase. p-Nitrophenyl-α-D-glucopyranoside and 2-chloro-4 nitrophenol-α-D- maltotrioside were used as the substrate for glucosidase and amylase respectively. Results: The dichloromethane fraction of the roots and stems from A. indicum and dichloromethane as well as methanolic fractions of the stems of M. coromandelianum could inhibit yeast alpha-glucosidase compared to 1-deoxynojirimycin with the IC50 of 0.36, 0.45, 0.48, 0.48 and 0.58 mg/ml respectively. A. indicum root methanolic fraction had the highest inhibitory effect on rat alpha-glucosidase activity compared to 1-deoxynojirimycin with the IC50 of 0.08 and 0.11 mg/ml respectively. M. coromandelianum, the dichloromethane fraction of roots and the methanolic fraction of stems, showed the strongest effect on alpha-amylase inhibition compared to acarbose with the IC50 of 0.07, 0.07 and 2.7 mg/ml, respectively. Conclusion: S. acuta, A. indicum and M. coromandelianum dichloromethane and methanolic fractions of the root, stem and leaf parts demonstrated an appreciable inhibitory activity on alpha-amylase from porcine, alpha-glucosidase from Saccharomyces cerevisiae and from rat intestine compared to 1-deoxynojirimycin and acarbose.

Download Full-text

Assessment of In Vitro Antidiabetic Potential of Purified Anthocyanin Extract from Floral Petals of Wild Balsam Species

Journal of Drug Delivery and Therapeutics ◽

10.22270/jddt.v10i3.4050 ◽

2020 ◽

Vol 10 (3) ◽

pp. 31-35

Author(s):

R ARATHY ◽

K MURUGAN ◽

KV DINESH BABU ◽

GS MANOJ

Keyword(s):

International Diabetes Federation ◽

Inhibitory Effect ◽

Alpha Amylase ◽

Herbal Remedies ◽

Antidiabetic Activity ◽

Public Health Issue ◽

Impatiens Balsamina ◽

Alpha Glucosidase

Diabetes is a notorious and growing clinical and public health issue. The International Diabetes Federation assumes that 592 million had diabetes by 2035 and that by 2040 the number will increase to 642 million. Cardiovascular corollary accounts for four million deaths annually attributable to diabetes. Evidence reveals that certain glucose-lowering phytochemicals can improve vascular outcomes with type 2 diabetes, which, together with better understanding of using multiple therapies concurrently, offers opportunities for beneficial personalization of medication regimens. Anthocyanins are coloured pigments and are natural antioxidants. Keeping this in focus, this study was undertaken to evaluate the in vitro antidiabetic activity in the petals of wild Impatiens balsamina L. The anthocyanin was extracted from floral petals of wild balsam species and purified to homogeneity using chromatographic techniques. Evaluation of in vitro antidiabetic properties of anthocyanin extract revealed a dose-dependent increase in the inhibitory effect on the alpha-glucosidase (200 μg/ml) and alpha-amylase enzymes (500 μg/ml) and was comparable with the standard acarbose drug (189 μg/ml and 50 μg/ml). These results indicated that anthocyanin could be used as a source of functional food and nutraceuticals. This information from wild species will be useful in finding more potent antidiabetic principle from the natural resources for the clinical development of antidiabetic therapeutics. Future studies are planned to substantiate the antidiabetic power of anthocyanin using in vivo animal models. Keywords: Alpha amylase, alpha glucosidase, diabetes, herbal remedies, Impatiens balsamina L.

Download Full-text