Peripheral blood and bone marrow findings in patients with acquired immune deficiency syndrome

Pathology ◽  
1990 ◽  
Vol 22 (4) ◽  
pp. 206-211 ◽  
Author(s):  
Catherine E. Harris ◽  
James C. Biggs ◽  
Alan J. Concannon ◽  
Anthony J. Dodds
Keyword(s):  
Immune Deficiency ◽  
Bone Marrow ◽  
Peripheral Blood ◽  
Immune Deficiency Syndrome ◽  
Acquired Immune Deficiency Syndrome ◽  
Deficiency Syndrome ◽  
Acquired Immune Deficiency

scholarly journals Decreased 5' nucleotidase activity in lymphocytes from asymptomatic sexually active homosexual men and patients with the acquired immune deficiency syndrome

Blood ◽  
1984 ◽  
Vol 64 (5) ◽  
pp. 1016-1021
Author(s):  
JL Murray ◽  
JM Reuben ◽  
CG Munn ◽  
PW Mansell ◽  
GR Newell ◽  
...  
Keyword(s):  
Immune Deficiency ◽  
Peripheral Blood ◽  
Mammalian Cells ◽  
Opportunistic Infections ◽  
Clinical Status ◽  
Immune Deficiency Syndrome ◽  
Acquired Immune Deficiency Syndrome ◽  
Deficiency Syndrome ◽  
Homosexual Men ◽  
Acquired Immune Deficiency

5′ Nucleotidase (5′NT) is an ectoenzyme associated with the plasma membrane of most mammalian cells. Low 5′NT activity has been observed in peripheral blood lymphocytes from patients with immunodeficiency states. 5′NT activity was measured in null and T-enriched lymphocytes from asymptomatic homosexual men and from 20 men with various degrees of the acquired immune deficiency syndrome (AIDS). Asymptomatic homosexuals were self-referred because of their concern about AIDS and were not necessarily representative of homosexuals in the general population. Enzyme activity was significantly decreased in both null (7.0 +/- 2.4 nmol/10(6) cells/h) and T-enriched (12.0 +/- 6.0 nmol/10(6) cells/h) lymphocytes in homosexuals as compared to lymphocytes from aged-matched heterosexual male and female controls (null = 10.8 +/- 6.5 and T = 22.3 +/- 10.6, P less than .0001 and .008, respectively). Decreased activity was present regardless of whether the patients were asymptomatic, had prodromal symptoms such as fever, lymph node enlargement, weight loss and diarrhea, or had opportunistic infections or Kaposi's sarcoma. Homosexuals had a significantly higher fraction of lymphocytes expressing the activation antigens T10 (20% +/- 3.3%) and Ia (13% +/- 2.9%) than controls (11% +/- 1.8% and 5% +/- 0.8%, respectively, P less than .05). They also had a significantly lower fraction of OKT4-positive helper lymphocytes than controls (22% +/- 3.4% v 35% +/- 2.2%, P less than .05). 5′NT activity in lymphocytes enriched for null cells from homosexuals correlated inversely with the percentage of Ia-positive lymphocytes (r = -.655; P less than .02). There was no correlation between 5′NT activity and the percentage of T4- or T8-positive lymphocytes or the T4/T8 ratio. Moreover, 5′NT activity was significantly decreased in both OKT4 (P less than .025) and OKT8 (P less than .05) enriched lymphocytes in homosexuals compared to controls. The data suggest that decreases in 5′NT may be a generalized defect of the peripheral blood T lymphocytes from active homosexuals that is independent of increases or decreases in specific T subpopulations or clinical status. It may contribute to the pathogenesis of AIDS.

scholarly journals Elevated adenosine deaminase and purine nucleoside phosphorylase activity in peripheral blood null lymphocytes from patients with acquired immune deficiency syndrome

Blood ◽  
1985 ◽  
Vol 65 (6) ◽  
pp. 1318-1324 ◽  
Author(s):  
JL Murray ◽  
KC Loftin ◽  
CG Munn ◽  
JM Reuben ◽  
PW Mansell ◽  
...  
Keyword(s):  
Immune Deficiency ◽  
Enzyme Activity ◽  
T Cell ◽  
Adenosine Deaminase ◽  
Peripheral Blood ◽  
Purine Nucleoside Phosphorylase ◽  
Immune Deficiency Syndrome ◽  
Acquired Immune Deficiency Syndrome ◽  
Deficiency Syndrome ◽  
Acquired Immune Deficiency

Abstract The purine metabolic enzymes adenosine deaminase (ADA) and purine nucleoside phosphorylase (PNP) are important in lymphocyte differentiation, and genetic deficiencies of either enzyme have been associated with hereditary immunodeficiency states. Both ADA and PNP activity were measured in null cell-enriched and T cell-enriched peripheral blood lymphocytes from 16 patients with the acquired immune deficiency syndrome (AIDS), seven patients with the AIDS-related symptom complex (ARC), and seven asymptomatic homosexuals. ADA activity in nmol/10(6) lymphocytes/h was significantly elevated in null lymphocytes from AIDS (161 +/- 12) as compared with 23 healthy heterosexual controls (127 +/- 8;P less than .025). PNP activity was also significantly increased in null lymphocytes from AIDS patients (96 +/- 10;P less than .005) as well as those from ARC patients (84 +/- 11:P less than .025) relative to controls (61 +/- 5). No significant differences in enzyme activity were noted in T cell-enriched cells in any group. Along with elevated enzyme activity, AIDS patients had small yet significant increases in the percentages of HLA-DR (P less than .025), terminal deoxynucleotidyl transferase (TdT) (P less than .0001), and peanut agglutinin receptor (P less than .0001) positive lymphocytes in the null fraction compared with controls. TdT-positive cells appeared morphologically as large lymphoblasts with irregular nuclei. The data imply that the cellular immune deficiency in AIDS is not a result of deficiencies in lymphocyte ADA or PNP activity, but is more likely associated with an increase in an immature and/or activated lymphocyte subset.

scholarly journals Decreased 5' nucleotidase activity in lymphocytes from asymptomatic sexually active homosexual men and patients with the acquired immune deficiency syndrome

Blood ◽  
1984 ◽  
Vol 64 (5) ◽  
pp. 1016-1021 ◽  
Author(s):  
JL Murray ◽  
JM Reuben ◽  
CG Munn ◽  
PW Mansell ◽  
GR Newell ◽  
...  
Keyword(s):  
Immune Deficiency ◽  
Peripheral Blood ◽  
Mammalian Cells ◽  
Opportunistic Infections ◽  
Clinical Status ◽  
Immune Deficiency Syndrome ◽  
Acquired Immune Deficiency Syndrome ◽  
Deficiency Syndrome ◽  
Homosexual Men ◽  
Acquired Immune Deficiency

Abstract 5′ Nucleotidase (5′NT) is an ectoenzyme associated with the plasma membrane of most mammalian cells. Low 5′NT activity has been observed in peripheral blood lymphocytes from patients with immunodeficiency states. 5′NT activity was measured in null and T-enriched lymphocytes from asymptomatic homosexual men and from 20 men with various degrees of the acquired immune deficiency syndrome (AIDS). Asymptomatic homosexuals were self-referred because of their concern about AIDS and were not necessarily representative of homosexuals in the general population. Enzyme activity was significantly decreased in both null (7.0 +/- 2.4 nmol/10(6) cells/h) and T-enriched (12.0 +/- 6.0 nmol/10(6) cells/h) lymphocytes in homosexuals as compared to lymphocytes from aged-matched heterosexual male and female controls (null = 10.8 +/- 6.5 and T = 22.3 +/- 10.6, P less than .0001 and .008, respectively). Decreased activity was present regardless of whether the patients were asymptomatic, had prodromal symptoms such as fever, lymph node enlargement, weight loss and diarrhea, or had opportunistic infections or Kaposi's sarcoma. Homosexuals had a significantly higher fraction of lymphocytes expressing the activation antigens T10 (20% +/- 3.3%) and Ia (13% +/- 2.9%) than controls (11% +/- 1.8% and 5% +/- 0.8%, respectively, P less than .05). They also had a significantly lower fraction of OKT4-positive helper lymphocytes than controls (22% +/- 3.4% v 35% +/- 2.2%, P less than .05). 5′NT activity in lymphocytes enriched for null cells from homosexuals correlated inversely with the percentage of Ia-positive lymphocytes (r = -.655; P less than .02). There was no correlation between 5′NT activity and the percentage of T4- or T8-positive lymphocytes or the T4/T8 ratio. Moreover, 5′NT activity was significantly decreased in both OKT4 (P less than .025) and OKT8 (P less than .05) enriched lymphocytes in homosexuals compared to controls. The data suggest that decreases in 5′NT may be a generalized defect of the peripheral blood T lymphocytes from active homosexuals that is independent of increases or decreases in specific T subpopulations or clinical status. It may contribute to the pathogenesis of AIDS.

Arteriopathy in Children with Acquired Immune Deficiency Syndrome

1987 ◽  
Vol 7 (3) ◽  
pp. 261-275 ◽  
Author(s):  
Vijay Joshi ◽  
Bruce Pawel ◽  
Edward Connor ◽  
Leroy Sharer ◽  
James Oleske ◽  
...  
Keyword(s):  
Immune Deficiency ◽  
Immune Deficiency Syndrome ◽  
Acquired Immune Deficiency Syndrome ◽  
Deficiency Syndrome ◽  
Acquired Immune Deficiency
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