Epidermal Growth Factor Content of Submandibular Glands is Increased in Rats with Experimentally Induced Gastric Lesions

The eyelid opening of pups born to and nursed by normal mice occurred by the 15th day of birth, whereas pregestational sialoadenectomy (removal of submandibular glands) of nursing mice delayed eyelid opening of their pups by as much as 5 days. Parotidectomy, however, had no effect on eyelid opening. Cross-foster nursing experiments indicated that the cause for delayed eyelid opening of pups was to be found in sialoadenectomized mothers, not their pups. Sialoadenectomized mothers had underdeveloped mammary glands that produced approximately 50% less milk than controls, and the amount of epidermal growth factor in their milk was similarly reduced. When epidermal growth factor, a polypeptide produced by the submandibular gland, was injected daily at a dose of 5 micrograms into sialoadenectomized pregnant mice, the eyelid opening of the pups nursed by their mothers occurred normally. These results are discussed with regard to the possible role of the submandibular gland and epidermal growth factor in neonatal eyelid opening.

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Mitogenic Activity and Epidermal Growth Factor Content in Human Milk

Acta Paediatrica ◽

10.1111/j.1651-2227.1985.tb10959.x ◽

1985 ◽

Vol 74 (2) ◽

pp. 250-253 ◽

Cited By ~ 25

Author(s):

L. JANSSON ◽

F. A. KARLSON ◽

B. WESTERMARK

Keyword(s):

Growth Factor ◽

Epidermal Growth Factor ◽

Human Milk ◽

Mitogenic Activity ◽

Factor Content ◽

Epidermal Growth

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Epidermal growth factor and prostaglandin E2 accelerate mucosal recovery from stress-induced gastric lesions via inhibition of apoptosis

Journal of Physiology-Paris ◽

10.1016/s0928-4257(01)00049-3 ◽

2001 ◽

Vol 95 (1-6) ◽

pp. 361-367 ◽

Cited By ~ 30

Author(s):

P.C Konturek ◽

T Brzozowski ◽

A Duda ◽

S Kwiecien ◽

S Löber ◽

...

Keyword(s):

Growth Factor ◽

Epidermal Growth Factor ◽

Prostaglandin E2 ◽

Gastric Lesions ◽

Epidermal Growth

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Influence of epidermal growth factor on liver regeneration after partial hepatectomy in mice

Journal of Endocrinology ◽

10.1677/joe.0.1280425 ◽

1991 ◽

Vol 128 (3) ◽

pp. 425-431 ◽

Cited By ~ 72

Author(s):

S. Noguchi ◽

Y. Ohba ◽

T. Oka

Keyword(s):

Growth Factor ◽

Epidermal Growth Factor ◽

Dna Synthesis ◽

Liver Regeneration ◽

Partial Hepatectomy ◽

Dna Content ◽

Sharp Peak ◽

Submandibular Glands ◽

Total Liver ◽

Epidermal Growth

ABSTRACT The role of epidermal growth factor (EGF) in liver regeneration was studied in mice after partial hepatectomy. Two weeks before partial hepatectomy, mice were sham-operated (control) or sialoadenectomized (removal of submandibular glands) to reduce plasma EGF levels. Sialoadenectomized mice showed low plasma EGF levels (29·7 ±6·6 pmol/l; mean ± s.e.m.) compared with controls (66·0±8·3 pmol/l). After partial hepatectomy, sialoadenectomized mice were treated with or without a daily s.c. injection of 5 μg EGF and the rate of DNA synthesis in the regenerating liver was monitored by [125I]iododeoxyuridine uptake. Control mice showed a sharp peak of DNA synthesis at 48 h after partial hepatectomy while sialoadenectomized mice showed a delayed and broad peak at 84 h. Treatment of sialoadenectomized mice with EGF (5 μg/mouse per day) completely restored the pattern of DNA synthesis so that a sharp peak appeared at 48 h. The total liver DNA content of the control mice (79·1±2·5% of the preoperative level; mean ± s.e.m.) was significantly (P < 0·01) higher than that of the sialoadenectomized mice (65·2±3·0%) 3 days after partial hepatectomy, but this difference disappeared on day 7 when liver regeneration was almost completed in both groups. Treatment of sialoadenectomized mice with EGF increased total liver DNA content (78·2±2·9%) to that of control mice on day 3 after partial hepatectomy. In addition, normal mice showed a rapid increase in plasma EGF levels at 1–8 h after partial hepatectomy, whereas sialoadenectomized mice showed low plasma EGF levels throughout the course of the experiment. These results suggest that EGF derived from the submandibular glands plays a role in promoting the early stage of liver regeneration. Journal of Endocrinology (1991) 128, 425–431

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