Human Lymphocyte Subpopulations: Rosette Formation with Sheep, Human and Horse Red Blood Cells

Abstract The occurrence of rosetting of Plasmodium falciparum-infected human red blood cells (IRBC) with uninfected red blood cells (RBC) and its potential pathophysiologic consequences were investigated under flow conditions using the perfused rat mesocecum vasculature. Perfusion experiments were performed using two knobby (K+) lines of P falciparum, ie, rosetting positive (K+R+) and rosetting negative (K+R-). The infusion of K+R+ IRBC resulted in higher peripheral resistance (PRU) than K+R- IRBC (P less than .0012). Video microscopy showed that under conditions of flow, in addition to cytoadherence of K+R+ IRBC to the venular endothelium, rosette formation was also restricted to venules, especially in the areas of slow flow. Rosettes were absent in arterioles and were presumably dissociated by higher wall shear rates. The presence of rosettes in the venules must therefore reflect their rapid reformation after disruption. Cytoadherence of K+R+ IRBC was characterized by formation of focal clusters along the venular wall. In addition, large aggregates of RBC were frequently observed at venular junctions, probably as a result of interaction between flowing rosettes, free IRBC, and uninfected RBC. In contrast, the infusion of K+R+ IRBC resulted in diffuse cytoadherence of these cells exclusively to the venular endothelium but not in rosetting or large aggregate formation. The cytoadherence of K+R+ IRBC showed strong inverse correlation with the venular diameter (r = -.856, P less than .00001). Incubation of K+R+ IRBC with heparin and with monoclonal antibodies to glycoprotein IV/CD36 abolished the rosette formation and resulted in decreased PRU and microvascular blockage. These findings demonstrate that rosetting of K+R+ IRBC with uninfected RBC enhances vasocclusion, suggesting an important in vivo role for rosetting in the microvascular sequestration of P falciparum-infected RBC.

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Human lymphocyte antibody-dependent cell-mediated cytotoxicity (ADCC) toward human red blood cells

Blood ◽

10.1182/blood.v52.4.696.696 ◽

1978 ◽

Vol 52 (4) ◽

pp. 696-705 ◽

Cited By ~ 17

Author(s):

GM Shaw ◽

PC Levy ◽

AF LoBuglio

Keyword(s):

Red Blood Cells ◽

Human Lymphocyte ◽

Blood Cells ◽

Human Red Blood Cells ◽

Dependent Cell

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The purification and kinetic properties of bisphosphoglycerate synthase from horse red blood cells

Archives of Biochemistry and Biophysics ◽

10.1016/0003-9861(76)90438-0 ◽

1976 ◽

Vol 177 (1) ◽

pp. 284-292 ◽

Cited By ~ 30

Author(s):

Zelda B. Rose ◽

Syamalima Dube

Keyword(s):

Red Blood Cells ◽

Blood Cells ◽

Kinetic Properties ◽

Horse Red Blood Cells

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The IgG Subclasses of Red Cell Antibodies and Relationship to Monocyte Binding

Blood ◽

10.1182/blood.v40.4.500.500 ◽

1972 ◽

Vol 40 (4) ◽

pp. 500-508 ◽

Cited By ~ 30

Author(s):

Neil Abramson ◽

Peter H. Schur

Keyword(s):

Red Blood Cells ◽

Blood Cells ◽

Igg Subclasses ◽

Red Cells ◽

Rosette Formation ◽

Igg Subclass ◽

Red Cell ◽

Myeloma Proteins ◽

Chromic Chloride

Abstract Purified IgG1, IgG2, IgG3, and IgG4 myeloma proteins were coupled to red blood cells with chromic chloride either individually or in various combinations. Only red blood cells coated with IgG1 or IgG3 formed rosettes; red cells coated with IgG3 appeared to predominate in this reaction. IgG2, IgG4, and IgA myeloma proteins coupled to red blood cells did not appear to inhibit the IgG1 and/or IgG3 also on the red cells from forming rosettes. The IgG subclass specificity of a number of red cell isoantibodies was determined. The majority were IgG1 and IgG3, as previously noted. IgG2 was observed among some of these antibodies, namely in anti-D, anti-CD, anti-CDE, anti-s, and anti-Jka. Although rosette formation was strongly associated with the relative amounts of IgG1 and IgG3, particularly the latter, on red cells, a number of exceptions were noted.

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