Evaluation of Cell Death in EB V-Transformed Lymphocytes Using Agarose Gel Electrophoresis, Light Microscopy and Electron Microscopy: II. Induction of Non-Classic Apoptosis (“Para-Apoptosis”) by Tritiated Thymidine

1995 ◽  
Vol 19 (1-2) ◽  
pp. 107-119 ◽  
Author(s):  
Eileen Asher ◽  
Claire M. Payne ◽  
Carol Bernstein
Keyword(s):  
Electron Microscopy ◽  
Cell Death ◽  
Light Microscopy ◽  
Gel Electrophoresis ◽  
Tritiated Thymidine ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis

Morphology of ricin and abrin exposed endothelial cells is consistent with apoptotic cell death

1996 ◽  
Vol 15 (5) ◽  
pp. 443-451 ◽  
Author(s):  
J Neil Hughes ◽  
Christopher D Lindsay ◽  
Gareth D Griffiths
Keyword(s):  
Cell Death ◽  
Gel Electrophoresis ◽  
Structural Changes ◽  
Apoptotic Cell ◽  
Nuclear Periphery ◽  
Neutral Red ◽  
Apoptotic Cell Death ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Cytoplasmic Structures

Cultures of bovine pulmonary endothelial (BPE) cells were exposed to LC70 doses of ricin or abrin (15.5 and 4.5 pM respectively) over a period of up to 40 h. The viability of the cultures (as determined by the neutral red (NR) dye retention assay) declined after 6 h exposure to the toxins. From 15 h onwards, cellular material in toxin exposed cultures became detached from the substra tum of the culture vessels. Hoffman modulation contrast photomicrography showed that this process was due to ricin and abrin exposed cells collapsing into membrane bound vesicles which retained the NR dye, became detached and floated into the medium. These apoptotic-like structural changes were further investi gated by transmission electron microscopy (TEM) and by agarose gel electrophoresis of DNA from control and exposed cultures. Many of the characteristic changes associated with apoptotic cell death were seen using TEM, including heterochromatin condensation at the nuclear periphery, crenulation of the nuclear mem brane and progressive degeneration of residual nuclear and cytoplasmic structures. The plasma membrane of many cells remained intact, and contained nuclear and cytoplasmic debris. Agarose gel electrophoresis of DNA extracted from toxin-treated cells revealed oligonucleosome sized DNA fragments, characteristic of apoptosis, from adherent cells at 7 h and both adherent and floating populations when harvested from 15 h; DNA from unexposed control cells did not show this fragmentation. The identification of apopto sis as being a significant additional mechanism of toxicity following exposure to ricin and abrin holotox ins raises the possibility of developing new therapeutic strategies against poisoning by these phytotoxins.

Gonadotropin suppression of apoptosis in follicle somatic cells

Zygote ◽  
1996 ◽  
Vol 4 (04) ◽  
pp. 299-303 ◽  
Author(s):  
G. Galeati ◽  
M. Forni ◽  
M. Spinaci
Keyword(s):  
Cell Death ◽  
Granulosa Cells ◽  
Gel Electrophoresis ◽  
Genomic Dna ◽  
Apoptotic Cell ◽  
Apoptotic Cell Death ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Follicular Atresia ◽  
Prominent Feature

In each oestrous cycle only a limited number of follicles are selected for ovulation whereas the remaining majority undergo atresia. The earliest and most prominent feature of atresia is the death of granulosa cells. Recent biochemical evidence has demonstrated that granulosa cell death during follicular atresia in swine (Tillyet al., 1992), bovine (Jollyet al., 1994) and rodent (Tillyet al., 1991) ovaries occurs by apoptosis, a process whereby cells die in a controlled manner. A biochemical event considered to be characteristic of apoptotic cell death is the intranucleosomal cleavage of genomic DNA into fragments 180–200 bp in size, which separate into a distinctive ladder-like pattern on agarose gel electrophoresis. Detection of this pattern of oligonucleosomes in DNA provides a marker of apoptotic cell death.

scholarly journals Apoptosis in the transplanted canine transmissible venereal tumor during growth and regression phases

2008 ◽  
Vol 60 (3) ◽  
pp. 607-612 ◽  
Author(s):  
F.G.A. Santos ◽  
A.C. Vasconcelos ◽  
J.E.S. Nunes ◽  
G.D. Cassali ◽  
T.A. Paixão ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Gel Electrophoresis ◽  
Genomic Dna ◽  
Methyl Green ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Transmission Electron ◽  
Tunel Reaction ◽  
Typical Morphology

Twelve male, mongrel, adult dogs were subcutaneously transplanted with cells originated from two canine transmissible venereal tumors (TVT). The aim was to demonstrate and to quantify the occurrence of apoptosis in the TVT regression. After six months of transplantation, a tumor sample was obtained from each dog, being six dogs with TVT in the growing phase and six in the regression phase as verified by daily measurements. Samples were processed for histological and ultrastructural purposes as well as for DNA extraction. Sections of 4µm were stained by HE, Shorr, methyl green pyronine, Van Gieson, TUNEL reaction and immunostained for P53. The Shorr stained sections went through morphometry that demonstrated an increase of the apoptotic cells per field in the regressive tumors. It was also confirmed by transmission electron microscopy, which showed cells with typical morphology of apoptosis and by the TUNEL reaction that detected in situ the 3'OH nick end labeling mainly in the regressive tumors. The regressive TVTs also showed an intensified immunostaining for P53 besides a more intense genomic DNA fragmentation detected by the agarose gel electrophoresis. In conclusion, apoptosis has an important role in the regression of the experimental TVT in a way that is P53-dependent.

Luminography – an Alternative Assay for Detection of von Willebrand Factor Multimers

1988 ◽  
Vol 60 (02) ◽  
pp. 133-136 ◽  
Author(s):  
R Schneppenheim ◽  
H Plendl ◽  
U Budde
Keyword(s):  
Von Willebrand Factor ◽  
Gel Electrophoresis ◽  
Detection Methods ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Luminescence Assay ◽  
Von Willebrand ◽  
Willebrand Factor

SummaryA luminescence assay was adapted for detection of von Willebrand factor multimers subsequent to SDS-agarose gel electrophoresis and electroblotting onto nitrocellulose. The method is as fast as chromogenic detection methods and appears to be as sensitive as autoradiography without the disadvantages of the latter.

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