A Modified Toluidine Blue Staining Method for Nucleoli of Ascites Tumor Cells

1974 ◽  
Vol 49 (4) ◽  
pp. 243-245 ◽  
Author(s):  
Antal Gyeskó ◽  
László Holczinger
Keyword(s):  
Tumor Cells ◽  
Toluidine Blue ◽  
Staining Method ◽  
Blue Staining ◽  
Ascites Tumor ◽  
Toluidine Blue Staining

scholarly journals A Modified Alcian Yellow and Toluidine Blue Staining Method to Visualize Intracellular Okadaella Gastrococcus

2011 ◽  
Vol 140 (5) ◽  
pp. S-318
Author(s):  
Takayuki Okada ◽  
Graham Adkins ◽  
Kazutoshi Hori ◽  
Hiroto Miwa
Keyword(s):  
Toluidine Blue ◽  
Staining Method ◽  
Blue Staining ◽  
Toluidine Blue Staining

Ultrafast Toluidine Blue Staining for Rapid On-Site Evaluation of Cytological Smears

2020 ◽  
Vol 64 (4) ◽  
pp. 375-377
Author(s):  
Ekkehard Hewer ◽  
Anja M. Schmitt
Keyword(s):  
Organic Solvents ◽  
Toluidine Blue ◽  
Critical Factor ◽  
Blue Staining ◽  
Nuclear Morphology ◽  
Hazardous Chemicals ◽  
Toluidine Blue Staining ◽  
Site Evaluation ◽  
The Rose

Rapid on-site evaluation (ROSE) is one of cytopathology’s “unique selling propositions.” The quality, speed, and ease of handling of the staining used is a critical factor for the efficacy of the ROSE procedure. Here, we describe a modification of rapid toluidine blue staining that can be performed within 25 s, provides excellent nuclear morphology, and is compatible with subsequent Papanicolaou staining of the slides. Furthermore, exposure to hazardous chemicals is minimized, as no organic solvents other than the alcohol-based fixative and glycerin for temporary mounting and coverslipping are required. We have used this protocol successfully in our ROSE practice and have not observed any discrepancies between toluidine blue- and permanent Papanicolaou-stained slides.

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