scholarly journals Evidencia de actividad feromonal de contacto en Cerambyx welensii Küster y compuestos candidatos

2020 ◽  
Vol 46 (1) ◽  
pp. 17-32
Author(s):  
María del Rocío López Manzano ◽  
Israel Sánchez Osorio ◽  
Gloria López Pantoja ◽  
Luis Domínguez Nevado ◽  
Gloria Rossell ◽  
...  
Keyword(s):  
Cerambyx Welensii

Este trabajo analizó la existencia potencial de feromona sexual de contacto en Cerambyx welensii. Muestras de élitros y protórax (micro extracción en fase sólida) de ambos sexos se analizaron mediante CG-MS. Para confirmar la existencia de feromona de contacto en la cutícula de las hembras, se estudió la respuesta de machos de C. welensii frente a: hembras intactas, hembras muertas, hembras sometidas a extracción hexánica de lípidos cuticulares y hembras recubiertas de su propi extracto hexánico cuticular. Se encontraron 44 hidrocarburos cuticulares en élitros y 17 en protórax. Los mismos 6 compuestos comprendieron en conjunto, tanto en élitros como en protórax, entre el 68.1% (élitros de machos) y el 85.4% (protórax de hembras) del contenido lipídico total. Ninguno de los compuestos resultó específico de las hembras, pero se encontraron diferencias entre sexos en la presencia relativa para algunos compuestos. Los compuestos candidatos para actuar como feromona de contacto podrían localizarse tanto en élitros como en protórax, pudiendo actuar como componentes mayoritarios (abundancias relativas >17% en MEFS) el 11-Me-C29, C27, 2-Me-C26 y 11/13-Me-C27; como componentes minoritarios (abundancia relativa <2.5%) podrían ser candidatos el 11/12/13-Me-C26 y el C28. La mayoría de los machos (82%) mostró la secuencia de cópula característica en Cerambycidae: Contacto antenal – Giro - Lamido – Arqueo abdominal. La retirada de componentes cuticulares redujo fuertemente el interés de los machos (lamido y arqueo abdominal: <5%); la restitución de los extractos hexánicos devolvió la respuesta positiva en un 22% de los casos, indicando que la feromona de contacto se encontraba en el extracto cuticular.

scholarly journals Electroantennographic Responses of Cerambyx welensii Küster to Host-Related Volatiles

Forests ◽  
2021 ◽  
Vol 12 (9) ◽  
pp. 1168
Author(s):  
Israel Sánchez-Osorio ◽  
Raúl Tapias ◽  
Luis Domínguez ◽  
Gloria López-Pantoja ◽  
María del Mar González
Keyword(s):  
Host Choice ◽  
Mass Trapping ◽  
Oak Trees ◽  
Volatile Organic ◽  
Management Context ◽  
Host Tree Species ◽  
Wood Boring ◽  
Dose Dependent ◽  
Common Tree ◽  
Cerambyx Welensii

Wood-boring insects, such as Cerambyx welensii Küster, are involved in oak decline in Mediterranean areas. To advance our understanding of the olfactory perception of C. welensii, we recorded electroantennographic (EAG) responses from male and female antennae to 32 tree volatile organic compounds typical of emissions from its main Quercus L. hosts, and also analysed the dose-dependent response. Cerambyx welensii antennae responded to 24 chemicals. Eight odorants elicited the highest EAG responses (normalized values of over 98%): 1,8-cineole, limonene-type blend, β-pinene, pinene-type blend, sabinene, α-pinene, turpentine and (E)-2-hexenal. Cerambyx welensii exhibits a broad sensitivity to common tree volatiles. The high EAG responses to both limonene- and pinene-type blends suggest the detection of specific blends of the main foliar monoterpenes emitted by Q. suber L. and Q. ilex L. (limonene, α- and β-pinene, sabinene and myrcene), which could influence the intraspecific host choice by C. welensii, and in particular, females may be able to detect oak trees with a limonene-type chemotype. In addition, C. welensii showed high antennal activity to some odorants that characterize emissions from non-host tree species (1,8-cineole, β-pinene, α-pinene, turpentine, δ3-carene and camphene). The results obtained may be applicable to optimize monitoring and mass-trapping programmes in an integrated pest management context.

scholarly journals Monoterpene emission of Quercus suber L. highly infested by Cerambyx welensii Küster

2019 ◽  
Vol 76 (4) ◽  
Author(s):  
Israel Sánchez-Osorio ◽  
Gloria López-Pantoja ◽  
Raúl Tapias ◽  
Evangelina Pareja-Sánchez ◽  
Luis Domínguez
Keyword(s):  
Quercus Suber ◽  
Monoterpene Emission ◽  
Cerambyx Welensii

scholarly journals Correction to: Monoterpene emission of Quercus suber L. highly infested by Cerambyx welensii Küster

2020 ◽  
Vol 77 (1) ◽  
Author(s):  
Israel Sánchez-Osorio ◽  
Gloria López-Pantoja ◽  
Raúl Tapias ◽  
Evangelina Pareja-Sánchez ◽  
Luis Domínguez
Keyword(s):  
Quercus Suber ◽  
Monoterpene Emission ◽  
Cerambyx Welensii

Assessing mass trapping efficiency and population density of Cerambyx welensii Küster by mark-recapture in dehesa open woodlands

2011 ◽  
Vol 131 (4) ◽  
pp. 1103-1116 ◽  
Author(s):  
Luis M. Torres-Vila ◽  
Álvaro Sanchez-González ◽  
Francisco Ponce-Escudero ◽  
Daniel Martín-Vertedor ◽  
Juan J. Ferrero-García
Keyword(s):  
Population Density ◽  
Trapping Efficiency ◽  
Mass Trapping ◽  
Mark Recapture ◽  
Cerambyx Welensii

scholarly journals Characterization of Actinomycetes Strains Isolated from the Intestinal Tract and Feces of the Larvae of the Longhorn Beetle Cerambyx welensii

2020 ◽  
Vol 8 (12) ◽  
pp. 2013
Author(s):  
Ramón I. Santamaría ◽  
Ana Martínez-Carrasco ◽  
Ricardo Sánchez de la Nieta ◽  
Luis M. Torres-Vila ◽  
Raúl Bonal ◽  
...  
Keyword(s):  
Natural Products ◽  
Intestinal Tract ◽  
Micrococcus Luteus ◽  
Hydrolytic Activity ◽  
Gram Positive ◽  
Genus Streptomyces ◽  
Gram Positive Bacteria ◽  
Longhorn Beetle ◽  
Cerambyx Welensii

Actinomycetes constitute a large group of Gram-positive bacteria present in different habitats. One of these habitats involves the association of these bacteria with insects. In this work, we have studied twenty-four actinomycetes strains isolated from the intestinal tract and feces from larvae of the xylophagous coleopteran Cerambyx welensii and have shown that seventeen strains present hydrolytic activity of some of the following substrates: cellulose, hemicellulose, starch and proteins. Fourteen of the isolates produce antimicrobial molecules against the Gram-positive bacteria Micrococcus luteus. Analysis of seven strains led us to identify the production of a wide number of compounds including streptanoate, alpiniamide A, alteramides A and B, coproporphyrin III, deferoxamine, demethylenenocardamine, dihydropicromycin, nocardamine, picromycin, surugamides A, B, C, D and E, tirandamycins A and B, and valinomycin. A significant number of other compounds, whose molecular formulae are not included in the Dictionary of Natural Products (DNP), were also present in the extracts analyzed, which opens up the possibility of identifying new active antibiotics. Molecular identification of ten of the isolated bacteria determined that six of them belong to the genus Streptomyces, two of them are included in the genus Amycolatopsis and two in the genus Nocardiopsis.

DNA barcoding of large oak-living cerambycids: diagnostic tool, phylogenetic insights and natural hybridization between Cerambyx cerdo and Cerambyx welensii (Coleoptera: Cerambycidae)

2018 ◽  
Vol 109 (05) ◽  
pp. 583-594 ◽  
Author(s):  
L.M. Torres-Vila ◽  
R. Bonal
Keyword(s):  
Dna Barcoding ◽  
Interspecific Cross ◽  
Gene Tree ◽  
Research Management ◽  
Natural Hybridization ◽  
Index Number ◽  
Rrna Gene ◽  
28S Rrna ◽  
Cerambyx Cerdo ◽  
Cerambyx Welensii

AbstractThree large saproxylic cerambycids with different pest/legal status co-occur in the Iberian oak woodlands, Cerambyx welensii (Cw), Cerambyx cerdo (Cc) and Prinobius myardi (Pm): Cw is an emerging pest, Cc is a protected but sometimes harmful species and Pm is a secondary/minor pest. A precise taxonomic diagnosis is necessary for research, management or protection purposes, but may be problematic mainly because Cw and Cc larvae are morphologically indistinguishable. To resolve this constraint, we genotyped adults, larvae and eggs collected over a wide geographical range using the mitochondrial barcoding of the cytochrome c oxidase subunit I (COI). A Neighbour-Joining tree phylogram revealed three distinct clusters corresponding to Cw, Cc and Pm. We further first sequenced for Cw and Cc two mitochondrial (12S rRNA and 16S rRNA) and one nuclear (28S rRNA) gene fragments. For the first two genes, interspecific divergence was lower than in COI, and for the 28S (lower mutation rate), the two species shared identical haplotypes. Two approaches for species delimitation (General Mixed Yule Coalescent (GMYC), Barcode Index Number (BIN)) confirmed the species distinctiveness of Cc and Cw. The Bayesian COI gene tree showed a remarkable genetic divergence between Cc populations from Iberia and the rest of Europe. Such divergence has relevant taxonomic connotations and stresses the importance of a wide geographical scale sampling for accurate DNA barcoding species identification. Incongruities between morphology/lineage and COI barcodes in some individuals revealed natural hybridization between Cw and Cc. Natural hybridization is important from a phylogenetic/evolutionary perspective in these cerambycids, but the prevalence of (and the behavioural/ecological factors involved in) interspecific cross-breeding remain to be investigated.

Ecology of Oobius rudnevi , egg parasitoid of Cerambyx cerdo and Cerambyx welensii in oak forests

2021 ◽  
Author(s):  
Luis M. Torres‐Vila ◽  
Rafael López‐Calvo ◽  
Álvaro Sánchez‐González ◽  
F. Javier Mendiola‐Díaz
Keyword(s):  
Egg Parasitoid ◽  
Oak Forests ◽  
Cerambyx Cerdo ◽  
Cerambyx Welensii

Mark-recapture estimates of the survival and recapture rates of Cerambyx welensii Küster (Coleoptera cerambycidae) in a cork oak dehesa in Huelva (Spain)

2008 ◽  
Vol 3 (4) ◽  
pp. 431-441 ◽  
Author(s):  
Gloria López-Pantoja ◽  
Luis Nevado ◽  
Israel Sánchez-Osorio
Keyword(s):  
Survival Rates ◽  
Potential Effect ◽  
Cork Oak ◽  
Female Group ◽  
Doñana National Park ◽  
Apparent Survival ◽  
Community Interest ◽  
Capture Recapture ◽  
Cerambyx Welensii

AbstractThe oak decline is probably the most severe plant health problem faced in the Mediterranean region which is one of the habitats of community interest under the EU’s environmental legal regime. More information on the role of Cerambycids species in this decay is still needed. This paper reports the apparent survival rate (Phi) and recapture rate (P) for a population of Cerambyx welensii Küster (Coleoptera cerambycidae) in a highly degraded cork oak grove near the Doñana National Park (Huelva, Spain) as calculated using the mark-capture-recapture method. High and constants in the time values of apparent survival rates for males and females are detected. The male overall recapture rate (P) exceeded that of the female group with relatively low, but significant, values. The presence of transient individuals suggests a nucleus of population with many immigrants and emigrants in the study plot. The results are used to discuss various aspects of the insect biology, and the potential effect of the gradual deterioration of the studied ecosystem on theinsect population it supports.

scholarly journals Primera aproximación al estudio de la actividad fisiológica en Quercus suber con alta presencia de Cerambyx welensi Küster

2020 ◽  
Vol 46 (1) ◽  
pp. 57-70
Author(s):  
Israel Sánchez Osorio ◽  
Gloria López Pantoja ◽  
Raúl Tapias Martín ◽  
Evangelina Pareja Sánchez ◽  
Luis Domínguez Nevado
Keyword(s):  
Quercus Suber ◽  
Cerambyx Welensii

La incidencia de Cerambyx welensii en Quercus suber es un factor clave en el decaimiento de árboles en las dehesas. Las pautas de selección de hospedantes por C. welensii son poco conocidas; entre otros factores, los volátiles de plantas se consideran claves en la localización de hospedantes en cerambícidos. En el presente trabajo estudiamos algunas características morfológicas de los árboles, así como la variación a corto plazo en la actividad fisiológica en Q. suber con distintos niveles de presencia de adultos de C. welensii. Nuestro objetivo fue analizar si la actividad fisiológica de Q. suber en condiciones de estrés veraniego podría variar entre árboles con distinta preferencia por C. welensii. Se estudiaron 36 Q. suber (18 árboles muy frecuentados por C. welensii; 18 sin avistamientos del insecto), en los cuales se midió la fotosíntesis neta, conductancia estomática y transpiración durante un período previo e incluyendo el inicio de vuelo diario de C. welensii (19:00 – 21:20 h); se analizó además el perímetro y la superficie de proyección de copa de los árboles. Los árboles con C. welensii exhibieron tasas fotosintéticas más altas (1.5–2.15 veces) que los árboles sin avistamientos del insecto entre las 19:35 y las 20:45, así como mayor perímetro y menor cociente superficie de proyección de copa a perímetro. Los resultados sugieren que tanto los ajustes fisiológicos bajo estrés ambiental (influyendo en el patrón de emisión de monoterpenos) como las características morfológicas de los árboles podrían tener un efecto en la selección intraespecífica de hospedantes por C. welensii.

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