Explaining trans-phase potential differences with membrane theory, association-induction hypothesis and murburn concept

The characteristics of the experimentally measured trans-membrane potential (TMP) generated across an artificial membrane intervening two KCl solutions were found to be explicable using simple principles of electrochemistry, as given within the context of Association Induction Hypothesis (AIH). AIH suggests that the heterogeneous ion distribution which is caused by the adsorption of a mobile ion onto an immobile phase (bearing charge opposite to that of the mobile ion) is responsible for the TMP generation. Therefore, this work proposes AIH could be an important foundation for explaining the origin of TMP. Our experimental observation of nonzero TMP across an electrically charged non-biological/synthetic membrane is found to be intriguing, as such outcomes are classically associated to ion-pumping activities of membrane proteins in a living matter. Another experimental observation of nonzero potential across a neutral membrane is even more intriguing. Such a potential behavior is more in harmony with murburn concept, a new proposal for explaining redox metabolic and physiological phenomena.

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Plasma Membrane Potential of Animal Cells Generated by Ion Pumping, Not by Ion Gradients

Ion Interactions in Energy Transfer Biomembranes ◽

10.1007/978-1-4684-8410-6_20 ◽

1986 ◽

pp. 183-193

Author(s):

C. L. Bashford ◽

C. A. Pasternak

Keyword(s):

Plasma Membrane ◽

Membrane Potential ◽

Animal Cells ◽

Plasma Membrane Potential ◽

Ion Gradients ◽

Ion Pumping

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Relationship between ion distribution and membrane potential during a steady state

The Bulletin of Mathematical Biophysics ◽

10.1007/bf02476718 ◽

1960 ◽

Vol 22 (4) ◽

pp. 323-349 ◽

Cited By ~ 4

Author(s):

Daniel L. Gilbert

Keyword(s):

Steady State ◽

Membrane Potential ◽

Ion Distribution

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A redox-generated biomimetic membrane potential across polypyrrole films

Chemical Communications ◽

10.1039/c9cc04021h ◽

2019 ◽

Vol 55 (67) ◽

pp. 10023-10026

Author(s):

Xiaoyan Nie ◽

Tianliang Xiao ◽

Zhaoyue Liu

Keyword(s):

Membrane Potential ◽

Artificial Membrane ◽

Polypyrrole Films ◽

Polypyrrole Film ◽

Biomimetic Membrane ◽

Anion Distribution ◽

Two Sides

An artificial membrane potential was generated through redox-regulating anion distribution on the two sides of a polypyrrole film.

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Ionic Regulation in the Muscle Fibres of Carcinus Maenas

Journal of Experimental Biology ◽

10.1242/jeb.32.2.383 ◽

1955 ◽

Vol 32 (2) ◽

pp. 383-396

Author(s):

J. SHAW

Keyword(s):

Membrane Potential ◽

Total Concentration ◽

Carcinus Maenas ◽

Chloride Ions ◽

Sartorius Muscle ◽

Muscle Fibres ◽

Ion Distribution ◽

Calcium And Magnesium ◽

Whole Muscle ◽

Total Muscle

1. Measurements have been made of the concentrations of potassium, chloride, calcium and magnesium, the conductivity and the membrane potential of single isolated fibres of the carpopodite extensor and flexor muscles of Carcinus maenas. 2. Analyses of whole muscles gave the total concentration of the cations as 224 mM./kg. H2O, of which potassium accounted for 120 mM./kg. and sodium 54 mM./kg. Of the anion fraction chloride only accounted for 54mM./kg. H2O. The analyses of the separated fibres were the same as for the whole muscle. 3. The average specific resistance of the fibres is 56Ω-cm. This represents a concentration of muscle ions of about 200 m.equiv./kg. and the electrolyte content of the muscle is not much more than a third of that of the blood. Between 72 and 91% of the total muscle fibre cations are present in an ionized form. 4. The average membrane potential is 58 mV. The ratios of the concentrations of potassium ions and chloride ions in the blood and muscle fibres suggest that these ions may be passively distributed across the membrane. The low concentration of sodium ions in the fibre probably indicates the operation of a ‘sodium pump’ as has been proposed for vertebrate muscles. The distribution of calcium and magnesium cannot be explained in simple terms. 5. The correspondence between the equilibrium potentials for potassium and chloride ions and the membrane potential suggests that theory of ion distribution put forward by Boyle & Conway for frog's sartorius muscle may also be applicable to Carcinus muscles.

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