scholarly journals Utility of scatterplot patterns of automated hematology analysers in white blood cell disorders – A comparative study with peripheral blood smears

2020 ◽  
Vol 11 (5) ◽  
pp. 29-34
Author(s):  
Tavish Gupta ◽  
Debdatta Basu
Keyword(s):  
Blood Cell ◽  
White Blood Cell ◽  
Peripheral Blood ◽  
Leucocyte Count ◽  
Scattered Light ◽  
Cell Counting ◽  
Total Leucocyte Count ◽  
Predictive Values ◽  
Cell Counts ◽  
Reference Plot

Background: Advances in automated blood cell counting offer new possibilities to analyse leukocyte differentials by fluorescence RNA/DNA staining combined with scattered light intensity technologies. Aims and Objectives: The purpose of this study is to identify the scatterplot patterns in normal cell counts and various White Blood Cell (WBC) disorders and to assess their efficacy compared to a peripheral blood smear (PBS) in the diagnosis of WBC disorders. Materials and Methods: A three-phased descriptive study analysing a total of 933 EDTA blood samples. The total leucocyte count, differential leucocyte count, scatterplot patterns as obtained by automated cell counter and PBS were studied in 405 normal samples (Phase 1) and 415 samples with WBC abnormalities (Phase 2). In phase 3, the scatterplots of 113 abnormal flagged samples were analysed and provisional diagnosis made in each case which was then compared with the diagnosis on the PBS. Sensitivity, Specificity, Positive and Negative predictive values (PPV & NPV) were calculated. Results: A grid-based standard scatterplot of normal samples was generated as a reference plot in phase one. Based on the scatterplots obtained in known WBC abnormalities studied in phase two, the basic abnormal patterns specific to each of the individual abnormalities were discerned. The phase three study showed excellent correlation between scatterplots and PBS findings in neutrophilia, eosinophilia, lymphocytosis and leukopenia (sensitivity 92-100%, specificity 98-100%). Abnormalities suspected to be leukemia on the scatterplots had a relatively low sensitivity and PPV. Conclusion: WBC scatterplots can be used as a screening tool as it correlates well with various WBC disorders in the PBS.

scholarly journals Microfluidic Cartridges for Automated, Point-of-Care Blood Cell Counting

2016 ◽  
Vol 22 (2) ◽  
pp. 176-185
Author(s):  
Suzanne Smith ◽  
Phophi Madzivhandila ◽  
René Sewart ◽  
Ureshnie Govender ◽  
Holger Becker ◽  
...  
Keyword(s):  
Blood Cell ◽  
White Blood Cell ◽  
Red Blood Cell ◽  
Blood Count ◽  
Point Of Care ◽  
Cell Counting ◽  
Full Blood Count ◽  
Diagnostic Tools ◽  
Cell Counts ◽  
Blood Cell Counting

Disposable, low-cost microfluidic cartridges for automated blood cell counting applications are presented in this article. The need for point-of-care medical diagnostic tools is evident, particularly in low-resource and rural settings, and a full blood count is often the first step in patient diagnosis. Total white and red blood cell counts have been implemented toward a full blood count, using microfluidic cartridges with automated sample introduction and processing steps for visual microscopy cell counting to be performed. The functional steps within the microfluidic cartridge as well as the surrounding instrumentation required to control and test the cartridges in an automated fashion are described. The results recorded from 10 white blood cell and 10 red blood cell counting cartridges are presented and compare well with the results obtained from the accepted gold-standard flow cytometry method performed at pathology laboratories. Comparisons were also made using manual methods of blood cell counting using a hemocytometer, as well as a commercially available point-of-care white blood cell counting system. The functionality of the blood cell counting microfluidic cartridges can be extended to platelet counting and potential hemoglobin analysis, toward the implementation of an automated, point-of-care full blood count.

An Alternative Elastase-mediated Degradation of Fibrinogen and Fibrin Observed in a Patient with Herpes Simplex Encephalitis and Pneumonia

1996 ◽  
Vol 76 (02) ◽  
pp. 184-186 ◽  
Author(s):  
Kenji lijima ◽  
Fumiyo Murakami ◽  
Yasushi Horie ◽  
Katsumi Nakamura ◽  
Shiro Ikawa ◽  
...  
Keyword(s):  
Herpes Simplex ◽  
Blood Cell ◽  
White Blood Cell ◽  
Herpes Simplex Encephalitis ◽  
Pmn Elastase ◽  
Cell Counts ◽  
Blood Cell Counts ◽  
Sds Page ◽  
White Blood Cell Counts ◽  
Pmn Élastase

SummaryA 74-year-old female developed pneumonia following herpes simplex encephalitis. Her white blood cell counts reached 28,400/μl, about 90% of which consisted of granulocytes. The polymorphonuclear (PMN) elastase/α1-arantitrypsin complex levels increased and reached the maximum of 5,019 ng/ml, indicating the release of a large amount of elastase derived from the granulocytes. The mechanism of PMN elastase release was most likely to be granulocyte destruction associated with phagocytosis. The cleavage of fibrinogen and fibrin by PMN elastase, independent of plasmin, was indicated by the presence of the fragments in immunoprecipitated plasma from the patient corresponding to elastase-induced FDP D and DD fragments and the absence of fragments corresponding to plasmin-induced FDP D and DD fragments on SDS-PAGE. These findings suggested that the large amount of PMN elastase released from the excessive numbers of granulocytes in this patient with herpes simplex encephalitis and pneumonia, induced the cleavage of fibrinogen and fibrin without the participation of plasmin.

Association Between White Blood Cell Counts and Brachial-Ankle Pulse Wave Velocity in Chinese Hypertensive Adults: A Cross-Sectional Study

Angiology ◽  
2021 ◽  
pp. 000331972110211
Author(s):  
Buyun Jia ◽  
Chongfei Jiang ◽  
Yun Song ◽  
Chenfangyuan Duan ◽  
Lishun Liu ◽  
...  
Keyword(s):  
Arterial Stiffness ◽  
Blood Cell ◽  
Pulse Wave Velocity ◽  
Wave Velocity ◽  
White Blood Cell ◽  
Pulse Wave ◽  
Final Analysis ◽  
Cross Sectional ◽  
Cell Counts ◽  
Wbc Count

Increased arterial stiffness is highly prevalent in patients with hypertension and is associated with cardiovascular (CV) risk. Increased white blood cell (WBC) counts may also be an independent risk factor for arterial stiffness and CV events. The aim of the study was to investigate the relationship between differential WBC counts and brachial-ankle pulse wave velocity (baPWV) in hypertensive adults. A total of 14 390 participants were included in the final analysis. A multivariate linear regression model was applied for the correlation analysis of WBC count and baPWV. Higher WBC counts were associated with a greater baPWV: adjusted β = 10 (95% CI, 8-13, P < .001). The same significant association was also found when WBC count was assessed as categories or quartiles. In addition, the effect of differential WBC subtypes, including neutrophil count and lymphocyte count on baPWV, showed the similar results. These findings showed that baPWV has positive associations with differential WBC counts in hypertensive adults.

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