scholarly journals In Vitro Regeneration of Phyllanthus amarus Schum. and Thonn.: An Important Medicinal Plant

Our Nature ◽  
1970 ◽  
Vol 7 (1) ◽  
pp. 110-115 ◽  
Author(s):  
A. Sen ◽  
M.M. Sharma ◽  
D. Grover ◽  
A. Batra
Keyword(s):  
In Vitro Regeneration ◽  
Multiple Shoots ◽  
Shoot Elongation ◽  
Nodal Segments ◽  
Nodal Segment ◽  
Phyllanthus Amarus ◽  
Regenerated Plants ◽  
Half Strength ◽  
Important Medicinal Plant

An efficient in vitro plant regeneration protocol was developed for the medicinally potent plant species Phyllanthus amarus Schum. and Thonn. (Euphorbiaceae) using nodal segment as explant. Maximum multiplication of shoots (15.275±0.96) was achieved on Murashige and Skoog’s medium supplemented with BAP (0.5 mg/l) after 3-4 weeks of inoculation. The shoots were separated from cluster and subcultured for their elongation on the same medium. In vitro flowering was also observed on the elongated shoots after 3–4 weeks of sub culturing on the shoot elongation medium. In vitro rooting was obtained on half strength MS medium supplemented with IBA (0.5 mg/l).  Regenerated plants were successfully hardened and acclimatized, 80 % of plantlets survived well under natural conditions after transplantation.Key words: In vitro regeneration, multiple shoots, nodal segments, Phyllanthus amarusDOI: 10.3126/on.v7i1.2557Our Nature (2009) 7:110-115

scholarly journals High Frequency In vitro Regeneration of Gynura procumbens (Lour.) Merr.

2017 ◽  
Vol 27 (2) ◽  
pp. 207-216
Author(s):  
Tanjina Akhtar Banu ◽  
Barna Goswami ◽  
Shahina Akter ◽  
Mousona Islam ◽  
Tammana Tanjin ◽  
...  
Keyword(s):  
In Vitro Regeneration ◽  
Leaf Explants ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
Nodal Segments ◽  
Nodal Segment ◽  
Root Induction ◽  
Petiole Explants ◽  
Number Of Shoots

An efficient rapid in vitro regeneration protocol was described from nodal segment, leaf and petiole explants. MS medium supplemented with 1.0 mg/l BAP and 0.5 mg/l IAA was found best for the multiple shoot formation from nodal segments. In this combination 99% explants produced multiple shoots and the average number of shoots per explants was 20.1 ± 1.96. For petiole and leaf explants best response was observed on MS supplemented with 2.0 mg/l BAP, 1 mg/l IAA and 0.5 mg/l Kn. Petiole explants produced highest mean number of shoots/explant (22.9 ± 1.728) among the three explants when the explants were cultured on MS with 2.0 mg/l BAP, 1 mg/l IAA and 0.5 mg/l Kn. The highest frequency of root induction (100%) and mean number of roots/plantlets (11.75) were obtained on MS. The rooted plantlets were transferred for hardening following acclimatization and finally were successfully established in the field.Plant Tissue Cult. & Biotech. 27(2): 207-216, 2017 (December)

scholarly journals In vitro propagation of Andrographis paniculata Nees.-A threatened medicinal plant of Bangladesh

2016 ◽  
Vol 3 (1) ◽  
pp. 67-73
Author(s):  
PK Roy
Keyword(s):  
Activated Charcoal ◽  
In Vitro Regeneration ◽  
Shoot Elongation ◽  
Shoot Tip ◽  
Andrographis Paniculata ◽  
Nodal Segment ◽  
Threatened Medicinal Plant ◽  
Half Strength ◽  
Number Of Shoots

An efficient protocol was developed for in vitro regeneration of plantlets from shoot tip and nodal segment explants of Andrographis paniculata Nees. Nodal segment explants produced the highest number of shoots (18±1.24) when they were cultured on MS supplemented with 11.10 ?M/l BAP. Addition of 10% coconut water and 2.0 g/l activated charcoal to the above mentioned medium increased the number of shoots (30) per culture. Shoot tip explant also showed better performance in the same medium. Addition of 100 mg/l urea and 2.0 g/l activated charcoal to the medium showed proper shoot elongation. The isolated shoots rooted well (90%) on half-strength MS fortified with 9.80 ?M/l IBA, where average number of roots per shoot was 28-30. The plantlets were acclimatized successfully in poly bags containing a mixture of soil, sand and compost in 2:1:1 ratio. Finally acclimatized plantlets were transferred to experimental field.Jahangirnagar University J. Biol. Sci. 3(1): 67-73, 2014 (June)

scholarly journals In vitro Shoot Proliferation and Plant Regeneration of Physalis minima L. - a Perennial Medicinal Herb

2010 ◽  
Vol 44 (4) ◽  
pp. 453-456 ◽  
Author(s):  
Farhana Afroz ◽  
AKM Sayeed Hassan ◽  
Laila Shamroze Bari ◽  
Rebeka Sultana ◽  
Nadira Begum ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Room Temperature ◽  
Shoot Proliferation ◽  
Nodal Segments ◽  
Nodal Segment ◽  
Ms Medium ◽  
Regenerated Plants ◽  
Half Strength ◽  
Shoot Tip Explants

The present study describes a protocol for high frequency plant regeneration of Physalis minima. Shoots were induced by culturing nodal segments and shoot tips from 15 day old seedlings. About 29 and 32 shoots were found to be induced from nodal segment and shoot tip explants, respectively, cultured on MS medium supplemented with 1.0 mg/l BAP. When shoots were subcultured on the fresh medium with same component as mentioned above, the shoots were elongated. Shoots rooted well when they were excised individually and implanted on half-strength MS medium with 0.3 mg/l NAA, where 98% shoots rooted within 12-15 days. In vitro grown plantlets with strong root system were successfully established in normal room temperature for seven days before transplanting in pots where they were reared for three weeks through successive acclimatization. The regenerated plants were successfully transferred to the soil with 90% survival rate. Key words: Physalis minima; Medicinal plant; Shoot proliferation; Micropropagation; Regeneration DOI: 10.3329/bjsir.v44i4.4597 Bangladesh J. Sci. Ind. Res. 44(4), 453-456, 2009

scholarly journals Micropropagation of Salvia broussonetii Benth. - A Medicinal Plant Species

1970 ◽  
Vol 16 (1) ◽  
pp. 19-23 ◽  
Author(s):  
S Mederos-Molina
Keyword(s):  
Ascorbic Acid ◽  
Medicinal Plant ◽  
Plant Species ◽  
Culture Medium ◽  
In Vitro Regeneration ◽  
Multiple Shoots ◽  
Axillary Shoots ◽  
Half Strength ◽  
Important Medicinal Plant

The purpose of this study was to establish culture medium requirements for micropropagation of Salvia broussonetii Benth., an important medicinal plant. Cultures were initiated from axillary shoots collected from mature plants. Most satisfactory results were achieved using a MS.2 medium supplemented with 1 mM ascorbic acid, 1.44 µM GA3 and 1.11 µM BAP. Axillary nodes were used for in vitro regeneration of multiple shoots and best results were achieved with MS.2 medium plus 1.44 µM GA3, 2.66 µM BAP and 1.14 µM IAA. Shoots rooted without symptoms of chlorosis or necrosis in half-strength MS.2 medium plus 1.44 µM GA3 and 2.28 µM IAA.Key words: Axillary shoots, Micropropagation, Medicinal plant, Salvia broussonetiiDOI = 10.3329/ptcb.v16i1.1101Plant Tissue Cult. & Biotech. 16(1): 19-23, 2006 (June)

scholarly journals Influence of Cytokinins in Combination with GA3on Shoot Multiplication and Elongation of Tea Clone Iran 100 (Camellia sinensis(L.) O. Kuntze)

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Reza Azadi Gonbad ◽  
Uma Rani Sinniah ◽  
Maheran Abdul Aziz ◽  
Rosfarizan Mohamad
Keyword(s):  
Camellia Sinensis ◽  
Woody Plants ◽  
Clonal Propagation ◽  
Shoot Multiplication ◽  
Shoot Elongation ◽  
Nodal Segments ◽  
Nodal Segment ◽  
Liquid Form ◽  
Solid Media

The use ofin vitroculture has been accepted as an efficient technique for clonal propagation of many woody plants. In the present research, we report the results of a number of experiments aimed at optimizing micropropagation protocol for tea (Camellia sinensis(L.) O. Kuntze) (clone Iran 100) using nodal segments as the explant. The effect of different combinations and concentrations of plant growth regulators (PGR) (BAP, TDZ, GA3) on shoot multiplication and elongation was assessed. The influence of exposure to IBA in liquid form prior to transfer to solid media on rooting of tea microshoots was investigated. The results of this study showed that the best treatment for nodal segment multiplication in terms of the number of shoot per explant and shoot elongation was obtained using 3 mg/L BAP in combination with 0.5 mg/L GA3. TDZ was found to be inappropriate for multiplication of tea clone Iran 100 as it resulted in hyperhydricity especially at concentrations higher than 0.05 mg/L. Healthy shoots treated with 300 mg/L IBA for 30 min followed by transfer to 1/2 strength MS medium devoid of PGR resulted in 72.3% of shoots producing roots and upon transferring them to acclimatization chamber 65% survival was obtained prior to field transfer.

scholarly journals An Efficient Protocol for High-frequency Direct Multiple Shoot Regeneration from Internodes of Peppermint (Mentha x piperita)

2010 ◽  
Vol 5 (12) ◽  
pp. 1934578X1000501
Author(s):  
Sanjog T. Thul ◽  
Arun K. Kukreja
Keyword(s):  
Shoot Regeneration ◽  
Multiple Shoots ◽  
Shoot Elongation ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Ms Medium ◽  
Mentha X Piperita ◽  
Half Strength ◽  
Multiple Shoot Regeneration

A simple, repeatable and efficient protocol for direct multiple shoot regeneration from internodal explants has been defined in peppermint ( Mentha x piperita var. Indus). In vitro regenerated shoots of peppermint were excised into 4 to 8 mm long internodes and cultured on Murashige and Skoog's medium supplemented with different cytokinins. In the hormonal assay, 3.0 mg L-l zeatin or 6-isopentenyl adenine independently supplemented to half strength MS medium exhibited multiple shoot regeneration, while thiaduzorn (0.1-3.0 mg L−1) showed no morphogenetic effect. A maximum of 85% in vitro cultured explants showed multiple shoot formation with an average of 7 shoots per explant on MS medium supplemented with zeatin. Multiple shoots were initiated within three weeks of cultivation. Internodes with regenerated multiple shoots were transferred to half - strength MS medium without supplementing with any plant growth hormone for shoot elongation and rhizogenesis. Rooted plants acclimatized and grew to maturity under glasshouse conditions. The plantlets developed were phenotypically identical to the parent plant and exhibited 96 % survival.

scholarly journals Types and concentrations of cytokinins in the micropropagation of Anthurium maricense

2018 ◽  
Vol 12 (2) ◽  
pp. 117
Author(s):  
Cecília Moreira Serafim ◽  
Arlene Santisteban Campos ◽  
Priscila Bezerra Dos Santos Melo ◽  
Ana Cecília Ribeiro de Castro ◽  
Ana Cristina Portugal Pinto de Carvalho
Keyword(s):  
Light Intensity ◽  
Culture Medium ◽  
Culture Media ◽  
In Vitro Regeneration ◽  
Nodal Segments ◽  
Nodal Segment ◽  
Viable Option ◽  
Growth Room ◽  
In Vitro Induction

Faced with the demand for plants potted for their foliage, Anthurium maricense is seen as a viable option. However, most of the studies on obtaining micropropagated plantlets are for A. andraeanum, with nothing yet reported for A. maricense. The aim of this study therefore, was to evaluate the effect of four cytokinins in different concentrations, on the in vitro induction of shoots from nodal segments of A. maricense. The experimental design was completely randomised in a 4 x 4 factorial scheme, with four cytokinins (BAP, ZEA, CIN and 2iP) and 4 concentrations (0, 2.22, 4.44 and 6.66 μM), for a total of 16 treatments, with 6 replications of five test tubes, and using one nodal segment. Cultures were kept in a growth room at 25 ± 2°C, a photoperiod of 16 h and a light intensity of 30 μmolm-2 s-1 for 60 days. After this period, the number of shoots formed per node was evaluated. The addition of a cytokinin to the culture medium was determinant for the in vitro regeneration of shoots in A. maricense. The greatest estimated number of shoot formations in A. maricense were obtained in the culture media containing ZEA (3.87) and BAP (3.30), both at concentration of 6.66 μM.

scholarly journals Plant Regeneration Through Nodal Explant Derived Callus in Wood Apple (Aegle marmelos L.)

1970 ◽  
Vol 44 (4) ◽  
pp. 415-420 ◽  
Author(s):  
Ranjoy Das ◽  
M Faruk Hasan ◽  
Harunar Rashid ◽  
Motiur Rahman
Keyword(s):  
Plant Regeneration ◽  
Callus Induction ◽  
Nodal Segments ◽  
Nodal Segment ◽  
Ms Medium ◽  
Nodal Explant ◽  
Aegle Marmelos ◽  
Half Strength ◽  
Subsequent Regeneration

This study reports on an improved protocol for callus induction and subsequent regeneration from nodal segment of wood apple (Aegle marmelos L.) Creamish friable competent callus was achieved from nodal segments on MS medium augmented with 4.0 mg1-1 2,4-D within two weeks of inoculation. The callus produced large number of shoots when cultured on MS medium fortified with 2.0 mgl-1 BAP+0.1 mgl-1 NAA within ten days of culture. In vitro raised shoots were rooted on half strength MS medium enriched with 1.0 mgl-1 IBA within fifteen days of culture. The rooted plantlets were successfully established with 80% survival. Key words: Plant regeneration; Callus induction; Nodal explant; Aegle marmelos. DOI: 10.3329/bjsir.v44i4.4590 Bangladesh J. Sci. Ind. Res. 44(4), 415-420, 2009

scholarly journals In Vitro Micropropagation of Stevia rebaudiana Bertoni

2019 ◽  
Vol 29 (2) ◽  
pp. 277-284
Author(s):  
Sabina Yesmin
Keyword(s):  
Morphological Variation ◽  
Stevia Rebaudiana ◽  
Multiple Shoots ◽  
Nodal Segments ◽  
Root Induction ◽  
Full Strength ◽  
Stevia Rebaudiana Bertoni ◽  
Regenerated Plants ◽  
In Vitro Micropropagation

Multiple shoots were obtained from both shoot tips and nodal segments cultured on MS fortified with different concentrations of BAP and Kn singly or in combination with low concentration of NAA. Maximum number of shoots (9.28 ± 0.61) were found on MS supplemented with 1.5 mg/l BAP and 05 mg/l NAA. In vitro regenerated shoots were separated and transferred onto half and full-strength MS supplemented with different concentration of IBA, IAA and NAA for root induction. Full strength MS containing 0.2 mg/l IBA was found to be best for root induction where 93.33% shoots were rooted. In vitro regenerated plants grew normally without showing any morphological variation and flowered after 40 days of transplantation.

scholarly journals An efficient in vitro regeneration protocol to generate stable transgenic lines of Black gram (Vigna mungo)

2018 ◽  
Vol 78 (3) ◽  
Author(s):  
Bidyut Kumar Sarmah ◽  
Trishna Konwar ◽  
Borsha Borah ◽  
Arun Kumar Handique ◽  
Sumita Acharjee
Keyword(s):  
Transformation Efficiency ◽  
In Vitro Regeneration ◽  
Multiple Shoots ◽  
Vigna Mungo ◽  
Ms Medium ◽  
Black Gram ◽  
Transgenic Lines ◽  
Half Strength ◽  
The Individual

An efficient and quick in vitro regeneration protocol was developed for black gram (Vigna mungo) using wounded embryonic axis with cotyledon as explant. Murashige and Skoog (MS) medium supplemented with 4.44 μM BAP and 2.32 μM Kinetin was found to be effective in producing maximum number (mean 7.80) of multiple shoots. The individual shoots elongated to 4.5 cm when MS medium was supplemented with 2.89 μM GA3 along with 0.44 μM BAP and 0.46 μM KIN. A novel in vitro rooting technique was also optimized for black gram using half-strength liquid MS medium supplemented with 1.34 μM NAA. The shoots in this medium produced the highest number (mean 7.50) of roots with root length of 6.02 cm. The plantlets were transferred to soil mixture and placed in greenhouse where more than 80% successfully grew to maturity. The same protocol was successfully used to generate transgenic black gram lines carrying Bt-Cry2Aa gene through Agrobacteriummediated transformation with a transformation efficiency of 0.42%. The rooted T0 plants grew to maturity and produced T1 seeds with the presence and expression of transgene in T1 plants. Thus, we have standardized an in vitro regeneration protocol suitable for generation of stable transgenic black gram plants.

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