scholarly journals Evaluation of the quality of embryo based on morphology cultured at low oxygen concentration

2021 ◽  
Vol 63 (9) ◽  
pp. 22-25
Author(s):  
Linh Chi Nguyen ◽  
◽  
Thi Tuong Chau Ngo ◽  
Dinh Tao Nguyen ◽  
Ngoc Diep Nguyen ◽  
...  
Keyword(s):  
Oxygen Concentration ◽  
Atmospheric Oxygen ◽  
Poor Quality ◽  
Embryo Morphology ◽  
Low Oxygen ◽  
Average Quality ◽  
Fertilisation Rate ◽  
Group 2 ◽  
Low Oxygen Concentration

Objectives: evaluating the quality of embryo morphology cultured at low oxygen concentration (5%) at different stages of embryonic development: day 3, day 5. Methods: the present study examined 168 IVF/ICSI cycles from October 2019 to February 2021 at the Assisted Reproductive Center, 16A Ha Dong General Hospital. Embryos were randomly assigned to 2 groups: group 1 used a K-system G-210 tri-gas incubator (Australia) (5% O2, 5% CO2, 90% N2) while group 2 used a Thermo Scientific 371 dual-gas incubator (Denmark) (5% CO2, 75% N2 with 20% atmospheric Oxygen concentration). Participants in the study were patients younger than 37 years old, with AMH>1.2 ng/ml, and AFC≥4. Mature oocytes were injected with sperm, and cultured in a sequential medium (G1,G2-PLUSTM). Embryologists assessed embryos on the day of fertilisation, days 3, 5, and compared the results of the two groups, using the method of descriptive statistics and T-test. The results revealed an insignificant difference in fertilisation rate and the quality of cleavages cultured in these 2 groups (percentages of good- and average quality cleavages were, in turn, 77.28±4.62% và 77.99±5.03%, at p>0.05, number of poor quality cleavages were, in turn, 1.71±0.38 vs 1.97±0.49 with p>0.05). The results on day 5 embryo showed that the percentage of blastocysts (from fertilisation) and the percentage of morphologically good- and average-quality blastocysts tended to increase higher when cultured in 5% oxygen concentration (p<0.05) compared with the 20% one (57.79±3.60% and 53.05±4.50%, 78.62±4.42% and 70.97±5.67%, respectively). Conclusions: embryo cultured in a low oxygen concentration helps embryos develop better on day 5 than when cultured at atmospheric concentrations

scholarly journals Effect of Initial Low Oxygen Concentration on Respiration and Quality of Fresh-cut Cabbages

10.5109/4543 ◽  
2003 ◽  
Vol 48 (1/2) ◽  
pp. 195-207
Author(s):  
Wenzhong Hu ◽  
Shun-ichiro Tanaka ◽  
Toshitaka Uchino ◽  
Toship Kawasaki ◽  
Daisuke Hamanaka ◽  
...  
Keyword(s):  
Oxygen Concentration ◽  
Low Oxygen ◽  
Fresh Cut ◽  
Low Oxygen Concentration

Effect of low oxygen concentration on activation of inflammation by Helicobacter pylori

2021 ◽  
Vol 560 ◽  
pp. 179-185
Author(s):  
Adiza Abass ◽  
Tokuju Okano ◽  
Kotchakorn Boonyaleka ◽  
Ryo Kinoshita-Daitoku ◽  
Shoji Yamaoka ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Oxygen Concentration ◽  
Low Oxygen ◽  
Low Oxygen Concentration

The Constant Low Oxygen Concentration in All the Target Cells for Mouse Tail Radionecrosis

1982 ◽  
Vol 92 (1) ◽  
pp. 172 ◽  
Author(s):  
J. H. Hendry ◽  
J. V. Moore ◽  
B. W. Hodgson ◽  
J. P. Keene
Keyword(s):  
Oxygen Concentration ◽  
Target Cells ◽  
Low Oxygen ◽  
Low Oxygen Concentration

scholarly journals Zooplankton fluctuations in Jurumirim Reservoir (São Paulo, Brazil): a three-year study

2009 ◽  
Vol 69 (1) ◽  
pp. 1-18 ◽  
Author(s):  
LP. Sartori ◽  
MG. Nogueira ◽  
R. Henry ◽  
EM. Moretto
Keyword(s):  
Water Column ◽  
Oxygen Concentration ◽  
Retention Time ◽  
Sao Paulo ◽  
Seasonal Effect ◽  
São Paulo ◽  
Low Oxygen ◽  
High Concentration ◽  
Heterogeneous Distribution ◽  
Low Oxygen Concentration

During three consecutive years, monthly samples of zooplankton were taken in the lacustrine (dam) zone of Jurumirim (São Paulo, Brazil). The seasonal effect on basic limnological features (thermal regime, oxygen distribution, phytoplankton biomass, etc.) was also examined. The influence of the seasonality on the fluctuation of the zooplankton composition and abundance was not clearly detected (low degree of recurrent patterns). Rotifers (32 taxa) were the most abundant organisms during almost the entire study period with some seasonal alternations in the maximum abundance peaks of the main taxa (Conochilus unicornis, Keratella americana, K. cochlearis and Hexarthra spp.), except for Polyarthra (mainly P. vulgaris). Only occasionally copepods were numerically dominant. Higher copepod abundance was positively associated to periods of increase in the water retention time. Among the Copepoda (10 taxa) the calanoids (mainly Notodiaptomus iheringi) were more abundant, especially in warmer periods. Conversely, cyclopoids had higher abundance in autumn and winter. The species Thermocyclops minutus and T. decipiens co-occurred, but the first attained higher abundance. Some evidence of co-existence strategies between both species are considered. Cladocera (17 taxa) was never numerically dominant and the main taxa (Bosmina spp., Ceriodaphnia spp. and Diaphanosoma spp.) occurred almost the whole study period and did not present a seasonal pattern of fluctuation. Diaphanosoma (mainly D. birgei) attained the highest abundance among cladocerans. Most organisms were always found at the surface, but they also occupy the whole water column, even in periods of stratified conditions and low oxygen concentration in the bottom layers. Among the main zooplanktonic taxa, only Hexarthra avoids deep layers. An exceptionally high concentration of Copepoda nauplii on the surface was influenced by low transparency, high concentration of phytoplankton at this layer and low oxygen concentration at the bottom. In periods of higher retention timevariability there was a more heterogeneous distribution of the zooplankton in the water column. The increase in the retention time seems also to favor the copepod development. Finally, some inter-decade changes are considered on the basis of zooplankton assemblage structure observations.

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