Aconitum uncinatum L., a herbaceous native, is not widely available for the public because of difficulties in producing plants via conventional methods. The initial objective was to find a growth regulator combination and an antioxidant concentration that provided increased proliferation rates. Growth regulator combinations examined were BA (0, 11.1, 22.2, 44.4 μm), 2iP (0, 24.6, 49.2 μm), kinetin (0, 23.2, 46.5 μm), IAA (0, 28.5, 57.1 μm), and NAA (0, 26.9, 53.71μm). Antioxidants examined were ascorbic acid (0, 150, 500 ppm), citric acid (0, 150, 500 ppm), PVP (0, 250, 500 ppm), PVPP (0, 250, 500 ppm), and MPP (0, 0.5,1 ppm). Surface response analysis was used to select 30 (of more than 3000 possible) combinations of growth regulator or antioxidant concentrations. Experiments were subcultured every 4 weeks and terminated at 8 weeks. Total-shoot proliferation was significantly greater on media containing 44.4 μm BA + 46.5 pm kinetin. There were six antioxidant combinations that were no different from the control (150 ppm each ascorbic acid and citric acid). Further experiments looking at various BA + kinetin and antioxidant concentrations are needed to refine the media for maximal proliferation.
SURFACE RESPONSE ANALYSIS OF A DIPPING LAYER BY RAY THEORY CONSIDERING EFFECT OF DIFFRACTED WAVES
