scholarly journals MICROPROPAGATION OF ACONZTUM UNCINATUM: GROWTH REGULATOR AND ANTIOXIDANT SCREENING USING THE SURFACE-RESPONSE ANALYSIS METHOD

HortScience ◽  
1995 ◽  
Vol 30 (2) ◽  
pp. 186a-186
Author(s):  
CC. Lim ◽  
S.L. Kitto
Keyword(s):  
Ascorbic Acid ◽  
Citric Acid ◽  
Growth Regulator ◽  
Shoot Proliferation ◽  
Response Analysis ◽  
Surface Response ◽  
The Media ◽  
Antioxidant Concentration ◽  
Initial Objective ◽  
Surface Response Analysis

Aconitum uncinatum L., a herbaceous native, is not widely available for the public because of difficulties in producing plants via conventional methods. The initial objective was to find a growth regulator combination and an antioxidant concentration that provided increased proliferation rates. Growth regulator combinations examined were BA (0, 11.1, 22.2, 44.4 μm), 2iP (0, 24.6, 49.2 μm), kinetin (0, 23.2, 46.5 μm), IAA (0, 28.5, 57.1 μm), and NAA (0, 26.9, 53.71μm). Antioxidants examined were ascorbic acid (0, 150, 500 ppm), citric acid (0, 150, 500 ppm), PVP (0, 250, 500 ppm), PVPP (0, 250, 500 ppm), and MPP (0, 0.5,1 ppm). Surface response analysis was used to select 30 (of more than 3000 possible) combinations of growth regulator or antioxidant concentrations. Experiments were subcultured every 4 weeks and terminated at 8 weeks. Total-shoot proliferation was significantly greater on media containing 44.4 μm BA + 46.5 pm kinetin. There were six antioxidant combinations that were no different from the control (150 ppm each ascorbic acid and citric acid). Further experiments looking at various BA + kinetin and antioxidant concentrations are needed to refine the media for maximal proliferation.

Poly-lactide-co-glycolide Microparticle Sizes: A Rational Factorial Design and Surface Response Analysis

2006 ◽  
Vol 6 (8) ◽  
pp. 2403-2407 ◽  
Author(s):  
Jocimara Ambrósio de Moraes Namur ◽  
Elaine Christine de Magalhães Cabral-Albuquerque ◽  
Wagner Quintilio ◽  
Maria Helena Andrade Santana ◽  
Mario José Politi ◽  
...  
Keyword(s):  
Factorial Design ◽  
Response Analysis ◽  
Surface Response ◽  
Surface Response Analysis

scholarly journals Utilization of the co-product of jaboticaba (Myrciaria jaboticaba Berg.) for pectin extraction

2020 ◽  
Vol 9 (6) ◽  
pp. e25963453
Author(s):  
Caroline Cagnin ◽  
Geovana Rocha Placido ◽  
Maisa Dias Cavalcante ◽  
Bheatriz Silva Morais de Freitas ◽  
Daniel Emanuel Cabral de Oliveira ◽  
...  
Keyword(s):  
Citric Acid ◽  
Acid Content ◽  
Galacturonic Acid ◽  
Food Industry ◽  
Response Analysis ◽  
Citric Acid Concentration ◽  
Pectin Content ◽  
Parameters Extraction ◽  
Neutral Sugars ◽  
Surface Response Analysis

Pectin is a polysaccharide that can be applied to various food industry products to modify the viscosity of food. The objective of this work was to optimize the extraction of pectin of jabuticaba peel by modificating the yield, galacturonic acid content (AUA), esterification content (DE), pectin content (P), and neutral sugars (N). The experiment was performed using the technique of factorial planning of complete experiments and surface response analysis. The extraction can be reported through factorial planning with coefficients of determination (R²) of 0.92, 0.91, 0.89, 0.88 and 0.90 for the yield extraction, AUA, DE, P, and N, respectively. The content of AUA ranged from 22.82 to 65.35%, the DE varied from 26.72 to 77.50%, the percentage of P found in the extraction 25.50 to 66.12%. The best performance was verified utilizing the following parameters: extraction time less than 75 min, temperature of 70°C, and citric acid concentration of 0.75 mol.L-1.

Effect of anti-browning compounds on the quality of minimally processed avocados / Efecto de inhibidores del pardeamiento en la calidad de aguacates mínimamente procesados

1998 ◽  
Vol 4 (2) ◽  
pp. 107-113 ◽  
Author(s):  
L. Dorantes-Alvarez ◽  
L. Parada-Dorantes ◽  
A. Ortiz-Moreno ◽  
T. Santiago-Pineda ◽  
A. Chiralt-Boix ◽  
...  
Keyword(s):  
Water Activity ◽  
The Other ◽  
Response Analysis ◽  
Minimally Processed ◽  
Tetrasodium Pyrophosphate ◽  
Surface Response ◽  
Surface Response Analysis

This work is concerned with the effect of anti-browning compounds (other than sulfites) on the preservation of minimally processed avocados. Ten different anti-browning agents were tested. After 5 days of storage results showed that cystein and tetrasodium pyrophosphate preserved better the original colour than the other agents used. Surface response analysis was used to monitor the effects of different mixtures of tetrasodium pyrophosphate/cystein, water activity, and pH of the immersing solution. It was found that one of the best combinations consisted of treatment with 1% tetrasodium pyrophosphate, 0.2% cystein at a water activity (a w) of 0.80 and pH 5.5. Selected formulations gave acceptable results for colour, flavour and strength of three minimally processed avocado slices samples, after 8 days of storage

Design of surface response analysis of KLa depending on aeration and agitation rate in a 14 L bioreactor

2010 ◽  
Author(s):  
F. Rodríguez-Gómez ◽  
M. Lozano-Contreras ◽  
M.G. Maldonado-Blanco ◽  
H.A. Luna-Olvera ◽  
C.F. Sandoval Coronado ◽  
...  
Keyword(s):  
Response Analysis ◽  
Agitation Rate ◽  
Surface Response ◽  
Surface Response Analysis

scholarly journals Optimization of dextran syntesis and acidic hydrolisis by surface response analysis

1999 ◽  
Vol 16 (2) ◽  
pp. 129-139 ◽  
Author(s):  
D.P. GUIMARÃES ◽  
F.A.A. COSTA ◽  
M.I. RODRIGUES ◽  
F. MAUGERI
Keyword(s):  
Response Analysis ◽  
Surface Response ◽  
Surface Response Analysis

scholarly journals Resolving browning during the establishment of explant cultures in Vicia faba L. for genetic transformation

2013 ◽  
Vol 61 (5) ◽  
pp. 1279-1288 ◽  
Author(s):  
Helena Klenotičová ◽  
Iva Smýkalová ◽  
Lenka Švábová ◽  
Miroslav Griga
Keyword(s):  
Ascorbic Acid ◽  
Citric Acid ◽  
Genetic Transformation ◽  
Faba Bean ◽  
Activated Charcoal ◽  
Shoot Growth ◽  
Shoot Proliferation ◽  
Cotyledonary Nodes ◽  
Regeneration Systems

Optimisation ofin vitroregeneration systems of two explant types for low-tannine cultivars of faba bean based on culturing of shoot apices and cotyledonary nodes were provided by usage of various antioxidants - ascorbic acid, citric acid, glutathione and activated charcoal. In subsequent testing, the combined effects of antioxidants with transformation co-cultivation compounds acetosyringone and L-cysteine was studied. The application of antioxidants lead to decreased callogenesis, citric acids treatments (50 mg.l−1) dramatically decreased necrotic response of explants. However, citric acid, used together with ascorbic acid completely inhibited shoot growth in shoot apex cultures. Glutathion evoked hyperhydricity of explants. Activated charcoal induced rooting on media which are commonly used for shoot proliferation. Combination of acetosyringone with antioxidants influenced shoot proliferation, except of variant with ascorbic acid. Citric acid was the best and universal antioxidant in faba beanin vitrocultures and its use is recommended for faba bean genetic transformation experiments.

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