scholarly journals Sororreatividade para Brucella abortus e Brucella ovis em pequenos ruminantes no Sertão de Itaparica, PE

2021 ◽  
Vol 3 (3) ◽  
pp. 111-117
Author(s):  
Maria de Fátima do Nascimento ◽  
Maviael Lopes Ferraz ◽  
Fabjane Samille da Silva ◽  
Edilma Zenilda de Souza ◽  
Marileide de Souza Sá ◽  
...  
Keyword(s):  
Brucella Abortus ◽  
Brucella Ovis

Objetivou-se realizar um levantamento soro-epidemiológico da brucelose nos rebanhos caprinos e ovinos no sertão de Itaparica. Foram coletadas amostras de 239 caprinos e 435 ovinos, totalizando 674 amostras, procedentes de 16 propriedades. As amostras foram obtidas por meio de venopunção da jugular, sendo acondicionadas em caixa de isopor com gelo para envio ao laboratório. Para a pesquisa de anticorpos anti-Brucella abortus, utilizou-se o teste do antígeno acidificado tamponado (TAAT) e a técnica de fixação do complemento. Neste trabalho, todas as amostras de soro caprino analisadas (n=161) mostraram resultado negativo no TAAT. Com relação à espécie ovina, das 343 amostras submetidas ao TAAT, observou-se uma soropositividade de 0,87% (n=03), contudo, essas amostras foram negativas no teste de fixação do complemento. A infecção natural pela Brucella abortus não ocorre nos rebanhos de caprinos e ovinos avaliados neste trabalho. Registra-se a soropositividade para Brucella ovis na espécie ovina, porém em baixa freqüência. Dessa forma, salienta-se a importância da continuidade de estudos como esse no Estado de Pernambuco e em outros estados geograficamente próximos, devido a importância econômica que a doença apresenta.

scholarly journals Brucella abortus RB51 and Hot Saline Extract from Brucella ovis as Antigens in a Complement Fixation Test Used To Detect Sheep Vaccinated with Brucella abortus RB51

2001 ◽  
Vol 8 (1) ◽  
pp. 119-122 ◽  
Author(s):  
Rosanna Adone ◽  
Franco Ciuchini
Keyword(s):  
Immune Responses ◽  
Brucella Abortus ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Infected Sheep ◽  
Fixation Test ◽  
Anticomplementary Activity ◽  
Serum Samples ◽  
Saline Extract ◽  
Brucella Ovis

ABSTRACT The efficacy of Brucella abortus RB51 and hot saline extract (HSE) from Brucella ovis as antigens in complement fixation (CF) tests was comparatively evaluated in detecting immune responses of sheep vaccinated with B. abortus strain RB51. For this study, four 5-month-old sheep were vaccinated subcutaneously with 5 × 109 CFU of RB51, and two sheep received saline. Serum samples collected at different times after vaccination were tested for the presence of antibodies to RB51 by a CF test with RB51 as antigen, previously deprived of anticomplementary activity, and with HSE antigen, which already used as the official antigen to detectB. ovis-infected sheep. The results showed that vaccinated sheep developed antibodies which reacted weakly against HSE antigen and these antibodies were detectable for 30 days after vaccination. However, antibodies to RB51 could be detected for a longer period after vaccination by using homologous RB51 antigen in CF tests. In fact, high titers were still present at 110 days postvaccination with RB51 antigen. Sera from sheep naturally infected with B. ovisalso reacted to RB51 but gave lower titers than those detected by HSE antigen. As expected, all sera from RB51-vaccinated sheep remained negative when tested with standard S-type Brucella standard antigens.

scholarly journals Genomic Island 2 Is an Unstable Genetic Element Contributing to Brucella Lipopolysaccharide Spontaneous Smooth-to-Rough Dissociation

2010 ◽  
Vol 192 (24) ◽  
pp. 6346-6351 ◽  
Author(s):  
Marcos Mancilla ◽  
Ignacio López-Goñi ◽  
Ignacio Moriyón ◽  
Ana María Zárraga
Keyword(s):  
Brucella Abortus ◽  
Genomic Island ◽  
Brucella Melitensis ◽  
Vaccine Production ◽  
Negative Bacterium ◽  
Integrase Gene ◽  
Brucella Ovis ◽  
Phage Integrase ◽  
Brucella Canis ◽  
Putative Phage

ABSTRACT Brucella is a Gram-negative bacterium that causes a worldwide-distributed zoonosis. The genus includes smooth (S) and rough (R) species that differ in the presence or absence, respectively, of the O-polysaccharide of lipopolysaccharide. In S brucellae, the O-polysaccharide is a critical diagnostic antigen and a virulence determinant. However, S brucellae spontaneously dissociate into R forms, a problem in antigen and S vaccine production. Spontaneous R mutants of Brucella abortus, Brucella melitensis, and Brucella suis carried the chromosomal scar corresponding to genomic island 2 (GI-2) excision, an event causing the loss of the wboA and wboB O-polysaccharide genes, and the predicted excised circular intermediate was identified in B. abortus, B. melitensis, and B. suis cultures. Moreover, disruption of a putative phage integrase gene in B. abortus GI-2 caused a reduction in O-polysaccharide loss rates under conditions promoting S-R dissociation. However, spontaneous R mutants not carrying the GI-2 scar were also detected. These results demonstrate that the phage integrase-related GI-2 excision is a cause of S-R brucella dissociation and that other undescribed mechanisms must also be involved. In the R Brucella species, previous works have shown that Brucella ovis but not Brucella canis lacks GI-2, and a chromosomal scar identical to those in R mutants was observed. These results suggest that the phage integrase-promoted GI-2 excision played a role in B. ovis speciation and are consistent with other evidence, suggesting that this species and B. canis have emerged as two independent lineages.

scholarly journals Inquérito soro-epidemiológico de Brucella ovis e Brucella abortus em rebanhos ovinos pertencentes à microrregião de Teresina, Brasil

PubVet ◽  
2017 ◽  
Vol 11 (5) ◽  
pp. 470-475 ◽  
Author(s):  
Gardenia Alves da Silva ◽  
Ana Gabriela Pereira Moura Leite ◽  
Letícia Soares de Araújo Teixeira ◽  
Estéfane Kelly Dias Araújo ◽  
Misael Santana das Virgens ◽  
...  
Keyword(s):  
Brucella Abortus ◽  
Brucella Ovis

scholarly journals Vaccination with Brucella abortus rough mutant RB51 protects BALB/c mice against virulent strains of Brucella abortus, Brucella melitensis, and Brucella ovis.

1994 ◽  
Vol 62 (11) ◽  
pp. 4990-4996 ◽  
Author(s):  
M P Jiménez de Bagüés ◽  
P H Elzer ◽  
S M Jones ◽  
J M Blasco ◽  
F M Enright ◽  
...  
Keyword(s):  
Brucella Abortus ◽  
Brucella Melitensis ◽  
Brucella Ovis ◽  
Virulent Strains ◽  
Rough Mutant

scholarly journals ATP-Binding Cassette Systems ofBrucella

2009 ◽  
Vol 2009 ◽  
pp. 1-16 ◽  
Author(s):  
Dominic C. Jenner ◽  
Elie Dassa ◽  
Adrian M. Whatmore ◽  
Helen S. Atkins
Keyword(s):  
Middle East ◽  
South America ◽  
Virulence Factors ◽  
Brucella Abortus ◽  
Brucella Melitensis ◽  
Human Brucellosis ◽  
Brucella Ovis ◽  
Brucella Canis ◽  
The World ◽  
Brucella Suis

Brucellosis is a prevalent zoonotic disease and is endemic in the Middle East, South America, and other areas of the world. In this study, complete inventories of putative functional ABC systems of fiveBrucellaspecies have been compiled and compared. ABC systems ofBrucella melitensis16M,Brucella abortus9-941,Brucella canisRM6/66,Brucella suis1330, andBrucella ovis63/290 were identified and aligned. High numbers of ABC systems, particularly nutrient importers, were found in allBrucellaspecies. However, differences in the total numbers of ABC systems were identified (B. melitensis, 79;B. suis, 72;B. abortus64;B. canis, 74;B. ovis, 59) as well as specific differences in the functional ABC systems of theBrucellaspecies. SinceB. ovisis not known to cause human brucellosis, functional ABC systems absent in theB. ovisgenome may represent virulence factors in human brucellosis.

scholarly journals The Outer Membrane of Brucella ovisShows Increased Permeability to Hydrophobic Probes and Is More Susceptible to Cationic Peptides than Are the Outer Membranes of Mutant Rough Brucella abortus Strains

1999 ◽  
Vol 67 (11) ◽  
pp. 6181-6186 ◽  
Author(s):  
Enrique Freer ◽  
Javier Pizarro-Cerdá ◽  
Andrej Weintraub ◽  
José-Antonio Bengoechea ◽  
Ignacio Moriyón ◽  
...  
Keyword(s):  
Outer Membrane ◽  
Brucella Abortus ◽  
Polymyxin B ◽  
Cationic Peptides ◽  
Morphological Alterations ◽  
Outer Membranes ◽  
Brucella Ovis ◽  
Culture Cells ◽  
Structural Differences ◽  
Hydrophobic Probe

ABSTRACT The permeability of the outer membrane (OM) to hydrophobic probes and its susceptibility to bactericidal cationic peptides were investigated for natural rough Brucella ovis and for mutant rough Brucella abortus strains. The OM of B. ovis displayed an abrupt and faster kinetic profile than roughB. abortus during the uptake of the hydrophobic probeN-phenyl-naphthylamine. B. ovis was more sensitive than rough B. abortus to the action of cationic peptides. Bactenecins 5 and 7 induced morphological alterations on the OMs of both rough Brucella strains. B. ovislipopolysaccharide (LPS) captured considerably more polymyxin B than LPSs from both rough and smooth B. abortus strains. Polymyxin B, poly-l-lysine, and poly-l-ornithine produced a thick coating on the surfaces of both strains, which was more evident in B. ovis than in rough B. abortus. The distinct functional properties of the OMs of these two rough strains correlate with some structural differences of their OMs and with their different biological behaviors in animals and culture cells.

scholarly journals The CO2-dependence of Brucella ovis and Brucella abortus biovars is caused by defective carbonic anhydrases

2018 ◽  
Vol 49 (1) ◽  
Author(s):  
Lara Pérez-Etayo ◽  
María Jesús de Miguel ◽  
Raquel Conde-Álvarez ◽  
Pilar M. Muñoz ◽  
Mammar Khames ◽  
...  
Keyword(s):  
Brucella Abortus ◽  
Carbonic Anhydrases ◽  
Brucella Ovis

scholarly journals Pesquisa de anticorpos anti-Brucella abortus e anti-Brucella ovis em ovinos no município de Uberlândia, MG

2011 ◽  
Vol 63 (4) ◽  
pp. 1022-1024 ◽  
Author(s):  
S.R.S. Salaberry ◽  
L.M. Paulin ◽  
R.L. Santana ◽  
J.R. Castro ◽  
A.M.C. Lima-Ribeiro
Keyword(s):  
Brucella Abortus ◽  
Brucella Ovis
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