scholarly journals Direct fluorescent antibody technique for the detection of bacterial kidney disease in paraffin-embedded tissues.

1985 ◽  
Vol 19 (4) ◽  
pp. 271-272 ◽  
Author(s):  
Tayehito OCHIAI ◽  
William T. YASUTAKE ◽  
Rowan W. GOLD
Keyword(s):  
Kidney Disease ◽  
Fluorescent Antibody ◽  
Fluorescent Antibody Technique ◽  
Antibody Technique ◽  
Bacterial Kidney Disease ◽  
Direct Fluorescent Antibody

Detection of Bacterial Kidney Disease in Wild Salmonids in the Margaree River System and Adjacent Waters Using an Indirect Fluorescent Antibody Technique

1979 ◽  
Vol 36 (12) ◽  
pp. 1464-1468 ◽  
Author(s):  
W. D. Paterson ◽  
C. Gallant ◽  
D. Desautels ◽  
L. Marshall
Keyword(s):  
Kidney Disease ◽  
Atlantic Salmon ◽  
Fluorescent Antibody ◽  
River System ◽  
Fluorescent Antibody Technique ◽  
Indirect Fluorescent Antibody ◽  
Antibody Technique ◽  
Bacterial Kidney Disease ◽  
Key Words Kidney ◽  
Asymptomatic Infections

The indirect fluorescent antibody technique demonstrated the enzootic nature of bacterial kidney disease in 224 of the 605 wild salmonids sampled from the Margaree River system and some adjacent waters. Asymptomatic infections were observed in 33.4% of 456 Atlantic salmon (Salmo salar) parr and 35.1% of 37 returning adults in samples taken from the source of the Margaree River to and including saltwater areas off the coast. Key words: kidney disease, Atlantic salmon, wild, distribution, fluorescent antibody

scholarly journals Immunoglobulins on the surface of blast cells in human acute leukemia

Blood ◽  
1975 ◽  
Vol 46 (3) ◽  
pp. 443-451
Author(s):  
RS Samoilova ◽  
TI Bulicheva ◽  
SV Skurkovich
Keyword(s):  
Lymphoblastic Leukemia ◽  
Fluorescent Antibody ◽  
Precursor Cell ◽  
Fluorescent Antibody Technique ◽  
Antibody Technique ◽  
Normal Number ◽  
Common Precursor ◽  
Direct Fluorescent Antibody ◽  
Blast Cells ◽  
Lymphocyte Populations

By means of the direct fluorescent antibody technique it was shown that malignant cells in acute lymphoblastic leukemia do not, as a rule, bear immunoglobulins on the cell surgace (43 of 45 cases). During complete remission the number of Ig-positive cells corresponds to the number of Ig-positive cells in normal lymphocyte populations. During incomplete remission as well as during incomplete remission as well as during intensive chemotherapy, the lymphocyte-like cells are evidently malignant, since they do not contain a normal number of Ig-positive lymphocytes. In acute myeloblastic leukemia (AML) and acute myelomonoblastic leukemia (AMML), lymphocytes distribute normally according to the number of Ig-positive and Ig-negative in 34 of 38 cases. AMML blast cells were Ig-positive in nine of ten cases. The data are discussed in the light of current concepts about the presence of a common precursor cell of granulocytes and monocytes.

scholarly journals Immunoglobulins on the surface of blast cells in human acute leukemia

Blood ◽  
1975 ◽  
Vol 46 (3) ◽  
pp. 443-451 ◽  
Author(s):  
RS Samoilova ◽  
TI Bulicheva ◽  
SV Skurkovich
Keyword(s):  
Lymphoblastic Leukemia ◽  
Fluorescent Antibody ◽  
Precursor Cell ◽  
Fluorescent Antibody Technique ◽  
Antibody Technique ◽  
Normal Number ◽  
Common Precursor ◽  
Direct Fluorescent Antibody ◽  
Blast Cells ◽  
Lymphocyte Populations

Abstract By means of the direct fluorescent antibody technique it was shown that malignant cells in acute lymphoblastic leukemia do not, as a rule, bear immunoglobulins on the cell surgace (43 of 45 cases). During complete remission the number of Ig-positive cells corresponds to the number of Ig-positive cells in normal lymphocyte populations. During incomplete remission as well as during incomplete remission as well as during intensive chemotherapy, the lymphocyte-like cells are evidently malignant, since they do not contain a normal number of Ig-positive lymphocytes. In acute myeloblastic leukemia (AML) and acute myelomonoblastic leukemia (AMML), lymphocytes distribute normally according to the number of Ig-positive and Ig-negative in 34 of 38 cases. AMML blast cells were Ig-positive in nine of ten cases. The data are discussed in the light of current concepts about the presence of a common precursor cell of granulocytes and monocytes.

Myxosoma cerebralis: Fluorescent Antibody Techniques for Antigen Recognition

1978 ◽  
Vol 35 (6) ◽  
pp. 828-832 ◽  
Author(s):  
Maria E. Markiw ◽  
Ken Wolf
Keyword(s):  
Life Cycle ◽  
Key Words ◽  
Fluorescent Antibody ◽  
Cross Reactivity ◽  
Antigen Recognition ◽  
Fluorescent Antibody Technique ◽  
Indirect Fluorescent Antibody ◽  
Whirling Disease ◽  
Antibody Technique ◽  
Direct Fluorescent Antibody

Rabbits were immunized with antigens extracted from mature spores or prespore stages of Myxosoma cerebralis, and the resulting antisera and their globulins were used in direct and indirect fluorescent antibody techniques. Both kinds of antisera reacted with homologous spores and with stages of the organism that precede spores. When tested for specificity against spores of 12 other myxosporidans the direct fluorescent antibody technique showed cross-reactivity with only one other and that was a species of Myxosoma. The indirect fluorescent antibody technique showed some reactions across generic lines. The antisera have application in studies of the parasite's life cycle and in diagnostics. Key words: spores, parasites, direct fluorescent antibody techniques, indirect fluorescent antibody techniques, diagnosis, myxosporidans, whirling disease

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