scholarly journals MICROSCOPÍA HOLOGRÁFICA DIGITAL APLICADA A LA DETECCIÓN DE MICROORGANISMOS MÓVILES DIGITAL HOLOGRAPHIC MICROSCOPY FOR MOTILE MICROORGANISMS DETECTION

Anales AFA ◽  
2010 ◽  
pp. 74-80
Author(s):  
A.C. Monaldi ◽  
G.G. Romero ◽  
E.E. Alanís ◽  
G.J. Curkovic
Keyword(s):  
Image Quality ◽  
Trypanosoma Cruzi ◽  
Digital Holographic Microscopy ◽  
Blood Samples ◽  
Holographic Image ◽  
Numerical Reconstruction ◽  
Reconstruction Methods ◽  
Holographic Microscopy ◽  
Set Up

In this work, the implementation of Digital Holographic Microscopy for detection of microorganisms in blood is proposed. Some hemoparasites, like Filaria and Trypanosoma Cruzi, can be identified in blood samples by their movement, which is oscillatory and propagates throughout the sample. Particularly, this behavior is considered for the T. Cruzi parasite movement characterization. By comparing two consecutive holograms, a final image of the vision field in which the parasite presence becomes evident is attained. The experimental set-up design for Digital Holographic Microscopy implementation is described and a discussion about the influence of the parameters required for holographic image quality is presented. The results obtained by different numerical reconstruction methods are compared.

scholarly journals Measuring the Cell-Induced Deformation of Collagen Matrix Detected With Digital Holographic Microscopy

2007 ◽  
Author(s):  
Kaveh Azartash ◽  
Enrico Gratton
Keyword(s):  
Mammalian Cells ◽  
Collagen Matrix ◽  
Spatial Arrangement ◽  
Digital Holographic Microscopy ◽  
Physical Aspect ◽  
Reconstruction Algorithms ◽  
Phase Images ◽  
Holographic Microscopy ◽  
Set Up ◽  
Charged Couple Device

A modified Mach-Zender set-up in reflection is applied to record and reconstruct holographic amplitude and phase images. A charged couple device (CCD) is used to record a hologram and numerical reconstruction algorithms are then applied to rebuild the hologram for obtaining both phase and amplitude information. One could also focus on multiple focal planes from a single hologram, similar to the focusing control of a conventional microscope. The morphology and behavior of mammalian cells is determined by an interaction between signals from the intracellular matrix and the cellular responses. It is important to note that the physical aspect of the extracellular matrix is as significant as the chemical nature of it. Specifically the stresses, mechanical forces, and the profile of the external environment have major effects on cell behavior. The mechanical and physical characteristics of a tissue are greatly dependent on a hierarchical spatial arrangement of its extra-cellular matrix components. A key player in the ECM is collagen which exhibits significant tensile strength on the cellular scale. Digital holographic microscopy (DHM) is applied to study the deformation of collage matrix in response to cell migration.

Effect of System Parameters on the Reconstructed Image Quality in Digital Holographic Microscopy

2011 ◽  
Vol 40 (2) ◽  
pp. 300-306 ◽  
Author(s):  
马利红 MA Li-hong ◽  
王辉 WANG Hui ◽  
李勇 YONG Li ◽  
邓丽军 DENG Li-jun
Keyword(s):  
Image Quality ◽  
Reconstructed Image ◽  
Digital Holographic Microscopy ◽  
System Parameters ◽  
Holographic Microscopy
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