In this work, the implementation of Digital Holographic Microscopy for detection of microorganisms in blood is
proposed. Some hemoparasites, like Filaria and Trypanosoma Cruzi, can be identified in blood samples by their
movement, which is oscillatory and propagates throughout the sample. Particularly, this behavior is considered
for the T. Cruzi parasite movement characterization. By comparing two consecutive holograms, a final image of
the vision field in which the parasite presence becomes evident is attained. The experimental set-up design for
Digital Holographic Microscopy implementation is described and a discussion about the influence of the
parameters required for holographic image quality is presented. The results obtained by different numerical
reconstruction methods are compared.
MICROSCOPÍA HOLOGRÁFICA DIGITAL APLICADA A LA
DETECCIÓN DE MICROORGANISMOS MÓVILES
DIGITAL HOLOGRAPHIC MICROSCOPY FOR MOTILE
MICROORGANISMS DETECTION