scholarly journals A New Assay Method for Anti-Acetylcholine Receptor(AChR) Antibodies in Sera from Patients with Myasthenia Gravis Using Anti-Torpedo californica AChR Antibody.

1995 ◽  
Vol 19 (1) ◽  
pp. 55-61 ◽  
Author(s):  
Mitsuhiro OHTA ◽  
Kyozo HAYASHI ◽  
Nobuyuki ITOH ◽  
Kiyoe OHTA
Keyword(s):  
Myasthenia Gravis ◽  
Acetylcholine Receptor ◽  
Assay Method ◽  
Torpedo Californica ◽  
Achr Antibody ◽  
Anti Acetylcholine

scholarly journals TE671 Cell-based ELISA for Anti-Acetylcholine Receptor Antibody Determination in Myasthenia Gravis

1999 ◽  
Vol 45 (3) ◽  
pp. 400-405 ◽  
Author(s):  
Diego Franciotta ◽  
Gianvito Martino ◽  
Elena Brambilla ◽  
Elisabetta Zardini ◽  
Vera Locatelli ◽  
...  
Keyword(s):  
Myasthenia Gravis ◽  
Acetylcholine Receptor ◽  
Achr Antibody ◽  
Te671 Cell ◽  
Two Samples ◽  
Human Muscles ◽  
Anti Acetylcholine ◽  
Good Agreement

Abstract Background: Acetylcholine receptor (AChR) from human muscles is the antigen used currently in radioimmunoprecipitation assays (RIPAs) for the determination of anti-AChR antibodies in the diagnosis of myasthenia gravis (MG). Our aim was to develop and validate an ELISA using TE671 cells as the source of AChR. Methods: After TE671 cell homogenization, the crude AChR extract was used for plate coating. Anti-AChR antibodies were determined in 207 MG patients and in 77 controls. Results: The mean intra- and interassay CVs (for two samples with different anti-AChR antibody concentrations) were 9.7% and 15.7%, respectively. Test sensitivity and specificity, for generalized MG, were 79.5% (95% confidence interval, 72.8–85.0%) and 96.1% (89.0–99.1%). The detection limit was 2 nmol/L. Anti-AChR antibody concentrations from 53 MG patients, as tested with our ELISA, showed good agreement with an RIPA with a mean difference (SD) of 1.0 (5.6) nmol/L. Conclusion: Our ELISA is a simple screening test for the diagnosis of MG and enables rapid and inexpensive patient follow-up.

Anti-acetylcholine receptor antibodies in neonatal myasthenia gravis: Heterogeneity and pathogenic significance

1991 ◽  
Vol 4 (2) ◽  
pp. 185-195 ◽  
Author(s):  
Bruno Eymard ◽  
Béatrice Vernet-der Garabedian ◽  
Sonia Berrih-Aknin ◽  
Claude Pannier ◽  
Jean-François Bach ◽  
...  
Keyword(s):  
Myasthenia Gravis ◽  
Acetylcholine Receptor ◽  
Receptor Antibodies ◽  
Anti Acetylcholine

scholarly journals A case of refractory generalized myasthenia gravis with anti-acetylcholine receptor antibodies treated with rituximab

2015 ◽  
Vol 55 (4) ◽  
pp. 227-232 ◽  
Author(s):  
Ryutaro Hayashi ◽  
Masayuki Tahara ◽  
Tomoko Oeda ◽  
Tetsuro Konishi ◽  
Hideyuki Sawada
Keyword(s):  
Myasthenia Gravis ◽  
Acetylcholine Receptor ◽  
Receptor Antibodies ◽  
Anti Acetylcholine

Intravenous γ-globulin in myasthenia gravis: Interaction with anti-acetylcholine receptor autoantibodies

1991 ◽  
Vol 11 (3) ◽  
pp. 128-131 ◽  
Author(s):  
R. Liblau ◽  
Ph. Gajdos ◽  
F. A. Bustarret ◽  
R. El Habib ◽  
J. F. Bach ◽  
...  
Keyword(s):  
Myasthenia Gravis ◽  
Acetylcholine Receptor ◽  
Anti Acetylcholine
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