Design of sEMG Acquisition Circuit and Its Adaptive EEMD Denosing Research

The Surface electromyography (sEMG) signal is a kind of electrical signal which generated by human muscles during contraction. It is prone to being affected by noise because of its small amplitude, so it is necessary to remove the noise in its original signal with an appropriate algorithm. Based on the traditional signal denoising indicators, a new complex indicator r has been proposed in this paper which combines three different indicator parameters, that is, Signal to Noise Ratio (SNR), correlation coefficient (R), and standard error (SE). At the same time, an adaptive ensemble empirical mode decomposition (EEMD) method named AIO-EEMD which based on the proposed indicator is represented later. To verify the effective of the proposed algorithm, an electromyography signal acquisition circuit is designed firstly for collecting the original sEMG signal. Then, the denosing performance from the designed method is been compared with empirical mode decomposition (EMD) method and wavelet transform noise reduction method, respectively. The experiment results shown that the designed algorithm can not only automatically get the numbers of the reconstructed signal numbers, but also obtain the best reduction performance.

Histamine Detection in Mackerel (Scomberomorus Sp.) and its Products Derivatized with 9-Flourenilmethylchloroformate

Histamine is commonly present in food containing proteins, like in mackerel. Consuming fish is imperative for the improvement of human muscles. Nevertheless, some studies reported ingesting fish containing histamine more than 50 mg·kg-1 can cause toxicity. This study analyzed and determined the composition of histamine in mackerel and its products commonly consumed in Malaysia, especially on the East Coast of Malaysia. These included processed mackerel such as canned products, satay (skewed fish) and keropok lekor (fish cake/ cracker). Histamine analysis was performed using High Performance Liquid Chromatography (HPLC) equipped with a fluorescence detector. A derivatizing reaction was applied to increase the sensitivity of HPLC to histamine using 9-flourenilmethylchloroformate (FMOC-Cl). The chromatographic separation was achieved in 15 min. Method validation was in accordance to Commission Decision 657/2002/CE. The linear range was at 0.16 – 5.00 µg·mL-1 (histamine) with the LOD at 0.10 µg·mL-1 and LOQ at 0.30 µg·mL-1 . Method applicability was checked on seven real samples involving raw, cooked, and dry products, yielding acceptable recovery.

Development of Transport for Disabled People on the Example of Wheelchair Propulsion with Cam-Thread Drive

The increasingly frequent use of electric drives is a new direction of development in personal transport. Sometimes these drives take over the work of human muscles, and sometimes they only support them. This is particularly evident in means of transport such as bicycles and scooters, but also in transporting people with disabilities. This study questions whether this is the only right development direction, and explores the possibility of developing means of transport for the more effective use of human muscles by proposing new structural solutions. We identified that such an action favors the minimization of the environmental load generated by technical facilities and, at the same time, may be a response to social needs resulting from the principles of sustainable development. This paper presents the operation principle of the innovative Wheelchair Cam-thread Drive (WCD), followed by field tests, laboratory measurements and biomechanical analyses of the WCD, comparing it with a typical Wheelchair Push-rim Drive (WPD). We found that the WCD allows efficient driving on flat and level surfaces, but its propulsion method can adversely alter the location of the center of gravity on the human-wheelchair system. A brake is also required to control the driving speed. Ultimately, the WCD was found to put less strain on the human movement system, so it could be used for rehabilitation exercises. The WCD appears to be a promising design, deserving further research into the drive biomechanics and the optimization of the mechanism operation. Such an innovative manual drive presents an interesting alternative to electric drives.

Distribution of motor unit properties across human muscles

The purpose of our review was to compare the distribution of motor unit properties across human muscles of different sizes and recruitment ranges. Although motor units can be distinguished based on several different attributes, we focused on four key parameters that have a significant influence on the force produced by muscle during voluntary contractions: the number of motor units, average innervation number, and the distributions of contractile characteristics and discharge rates within motor unit pools. Despite relatively few publications on this topic, current data indicate that the most influential factor in the distribution of these motor unit properties between muscles is innervation number. Nonetheless, despite a five-fold difference in innervation number between a hand muscle (first dorsal interosseus) and a lower leg muscle (tibialis anterior), the general organization of their motor unit pools and the range of discharge rates appears to be relatively similar. These observations provide foundational knowledge for studies on the control of movement and the changes that occur with ageing and neurological disorders.

The effect of stretch–shortening magnitude and muscle–tendon unit length on performance enhancement in a stretch–shortening cycle

Stretch-induced residual force enhancement (rFE) is associated with increased performance in a stretch–shortening cycle (SSC). Although the influence of different range of motions and muscle–tendon unit lengths has been investigated in pure stretch-hold experiments in vivo, the contribution to a SSC movement in human muscles remains unclear. In two sessions, 25 healthy participants performed isometric reference (ISO), shortening hold (SHO) and SSC contractions on an isokinetic dynamometer. We measured the net knee-joint torque, rotational mechanical work, knee kinematics and fascicle behavior (m. vastus lateralis) of the upper right leg. In session 1 the SHO- and SSC-magnitude was changed respectively (SHO: 50°–20°, 80°–20° and 110°–20°; SSC: 20°–50°–20°, 20°–80°–20° and 20°–110°–20°) and in session 2 the muscle–tendon unit length (SHO: 50°–20°, 80°–50° and 110°–80°; SSC: 20°–50°–20°, 50°–80°–50° and 80°–110°–80°; straight leg = 0°). In both sessions, rotational work was significantly (p < 0.05) increased in the SSC compared to the SHO contractions (in the range of 8.1–17.9%). No significant difference of joint torque was found in the steady-state for all SSC-magnitudes compared to the corresponding SHO contractions in session 1. In session 2, we found only significantly (p < 0.05) less depressed joint torque in the SSC at the longest muscle–tendon unit length compared to the corresponding SHO condition, without any differences in knee kinematics and fascicle behavior. Therefore, the physiological relevance of rFE might be particularly important for movements at greater muscle–tendon unit lengths.

Human myotube formation is determined by MyoD–Myomixer/Myomaker axis

Myoblast fusion is essential for formations of myofibers, the basic cellular and functional units of skeletal muscles. Recent genetic studies in mice identified two long-sought membrane proteins, Myomaker and Myomixer, which cooperatively drive myoblast fusion. It is unknown whether and how human muscles, with myofibers of tremendously larger size, use this mechanism to achieve multinucleations. Here, we report an interesting fusion model of human myoblasts where Myomaker is sufficient to induce low-grade fusion, while Myomixer boosts its efficiency to generate giant myotubes. By CRISPR mutagenesis and biochemical assays, we identified MyoD as the key molecular switch of fusion that is required and sufficient to initiate Myomixer and Myomaker expression. Mechanistically, we defined the E-box motifs on promoters of Myomixer and Myomaker by which MyoD induces their expression for multinucleations of human muscle cells. Together, our study uncovered the key molecular apparatus and the transcriptional control mechanism underlying human myoblast fusion.

Model Predictive Control for Modeling Human Gait Motions Assisted by Vicon Technology

Human muscles and the central nervous system (CNS) play the key role to control the human movements and activities. The human CNS determines each human motion following three steps: estimation of the movement trajectory; calculation of required energy for muscles; then perform the motion. In these three step tasks, the human CNS determines the first two steps and the human muscles conduct the third one. This paper efforts the use of model predictive control (MPC) algorithm to simulate the human CNS calculation in the case of gait motion. We first build up the human gait motion mathematical model with 5-link mechanism. This allows us to apply MPC to calculate the optimal torques at each joint and optimal trajectory for muscles. Outcomes of simulations simultaneously are compared with the real human movements captured by the Vicon motion capture technology which is the novelty of this study. Results show that tracking errors are not excessed 7%.

Age-Associated Salivary MicroRNA Biomarkers for Oculopharyngeal Muscular Dystrophy

Small non-coding microRNAs (miRNAs) are involved in the regulation of mRNA stability. Their features, including high stability and secretion to biofluids, make them attractive as potential biomarkers for diverse pathologies. This is the first study reporting miRNA as potential biomarkers for oculopharyngeal muscular dystrophy (OPMD), an adult-onset myopathy. We hypothesized that miRNA that is differentially expressed in affected muscles from OPMD patients is secreted to biofluids and those miRNAs could be used as biomarkers for OPMD. We first identified candidate miRNAs from OPMD-affected muscles and from muscles from an OPMD mouse model using RNA sequencing. We then compared the OPMD-deregulated miRNAs to the literature and, subsequently, we selected a few candidates for expression studies in serum and saliva biofluids using qRT-PCR. We identified 126 miRNAs OPMD-deregulated in human muscles, but 36 deregulated miRNAs in mice only (pFDR < 0.05). Only 15 OPMD-deregulated miRNAs overlapped between the in humans and mouse studies. The majority of the OPMD-deregulated miRNAs showed opposite deregulation direction compared with known muscular dystrophies miRNAs (myoMirs), which are associated. In contrast, similar dysregulation direction was found for 13 miRNAs that are common between OPMD and aging muscles. A significant age-association (p < 0.05) was found for 17 OPMD-deregulated miRNAs (13.4%), whereas in controls, only six miRNAs (1.4%) showed a significant age-association, suggesting that miRNA expression in OPMD is highly age-associated. miRNA expression in biofluids revealed that OPMD-associated deregulation in saliva was similar to that in muscles, but not in serum. The same as in muscle, miRNA expression levels in saliva were also found to be associated with age (p < 0.05). Moreover, the majority of OPMD-miRNAs were found to be associated with dysphagia as an initial symptom. We suggest that levels of specific miRNAs in saliva can mark muscle degeneration in general and dysphagia in OPMD.