Liver X receptor α promotes milk fat synthesis in buffalo mammary epithelial cells by regulating the expression of FASN

2021 ◽  
Author(s):  
Yongyun Zhang ◽  
Xinyang Fan ◽  
Lihua Qiu ◽  
Wei Zhu ◽  
Lige Huang ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Milk Fat ◽  
Mammary Epithelial Cells ◽  
Liver X Receptor ◽  
Mammary Epithelial ◽  
Liver X Receptor Α ◽  
Fat Synthesis ◽  
Milk Fat Synthesis

Liver X receptor α participates in LPS-induced reduction of triglyceride synthesis in bovine mammary epithelial cells

2020 ◽  
pp. 1-7
Author(s):  
Jianfa Wang ◽  
Shuai Lian ◽  
Jun Song ◽  
Hai Wang ◽  
Xu Zhang ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Epithelial Cells ◽  
Dairy Cow ◽  
Milk Fat ◽  
Mammary Epithelial Cells ◽  
Liver X Receptor ◽  
Mammary Epithelial ◽  
Triglyceride Synthesis ◽  
Milk Fat Depression ◽  
Liver X Receptor Α

Abstract Lipopolysaccharides (LPS) could induce milk fat depression via regulating the body and blood fat metabolism. However, it is not completely clear how LPS might regulate triglyceride synthesis in dairy cow mammary epithelial cells (DCMECs). DCMECs were isolated and purified from dairy cow mammary tissue and treated with LPS. The level of triglyceride synthesis, the expression and activity of the liver X receptor α (LXRα), enzymes related to de novo fatty acid synthesis, and the expression of the fatty acid transporters were investigated. We found that LPS decreased the level of triglyceride synthesis via a down-regulation of the transcription, translation, and nuclear translocation level of the LXRα. The results also indicated that the transcription level of the LXRα target genes, sterol regulatory element binding protein 1 (SREBP1), fatty acid synthetase (FAS), acetyl-CoA carboxylase-1 (ACC1), were significantly down-regulated in DCMECs after LPS treatment. Our data may provide new insight into the mechanisms of milk fat depression caused by LPS.

MicroRNA-432 inhibits milk fat synthesis by targeting SCD and LPL in ovine mammary epithelial cells

2021 ◽  
Author(s):  
Zhiyun Hao ◽  
Yuzhu Luo ◽  
Jiqing Wang ◽  
Jon Hickford ◽  
Huitong Zhou ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Epithelial Cells ◽  
Milk Production ◽  
Milk Fat ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Differentially Expressed ◽  
Differentially Expressed Mirnas ◽  
Fat Synthesis ◽  
Milk Fat Synthesis

In our previous studies, microRNA-432 (miR-432) was found to be one of differentially expressed miRNAs in ovine mammary gland between the two breeds of lactating sheep with different milk production...

Effect of INSIG1 on the milk fat synthesis of buffalo mammary epithelial cells

2020 ◽  
Vol 87 (3) ◽  
pp. 349-355
Author(s):  
Xinyang Fan ◽  
Lihua Qiu ◽  
Xiaohong Teng ◽  
Yongyun Zhang ◽  
Yongwang Miao
Keyword(s):  
Epithelial Cells ◽  
Milk Fat ◽  
Mammary Epithelial Cells ◽  
Strong Support ◽  
Mammary Epithelial ◽  
Mammary Tissue ◽  
Peak Period ◽  
Fat Synthesis ◽  
Milk Fat Synthesis

AbstractWe hypothesized that insulin-induced gene 1 (INSIG1) affects milk fat synthesis in buffalo. For this reason, the protein abundance of INSIG1 in the mammary tissue of buffalo during the peak period of lactation and dry-off period was evaluated. The results showed that the expression of INSIG1 at the peak of lactation was lower than that in the dry-off period. To explore the role of INSIG1 in milk fat synthesis, the buffalo mammary epithelial cells (BMECs) were isolated and purified from buffalo mammary tissue, and INSIG1 gene were overexpressed and knocked down by constructing the recombinant lentivirus vector of INSIG1 gene and transfecting into BMECs. Results revealed that INSIG1 overexpression decreased the expression of INSIG2, SREBP, PPARG, SCD, GPAM, DGAT2 and AGPAT6, which led to reduction of triglycerides (TAG) content in the cell. In contrast, knockdown of INSIG1 had a positive effect on mRNA expression of the above genes. Overall, the data provide strong support for a key role of INSIG1 in the regulation of milk fat synthesis in BMECs.

CRTC2 Is a Key Mediator of Amino Acid-Induced Milk Fat Synthesis in Mammary Epithelial Cells

2019 ◽  
Vol 67 (37) ◽  
pp. 10513-10520 ◽  
Author(s):  
Ping Li ◽  
Chengjian Zhou ◽  
Xueying Li ◽  
Mengmeng Yu ◽  
Meng Li ◽  
...  
Keyword(s):  
Amino Acid ◽  
Epithelial Cells ◽  
Milk Fat ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Fat Synthesis ◽  
Milk Fat Synthesis

Effects of Insulin on Milk Fat Synthesis by Regulating the Expressions of SREBP1 and mTOR in Bovine Mammary Epithelial Cells

2020 ◽  
Author(s):  
Nan Li ◽  
Peng-Xia Zhang ◽  
Xin Huang ◽  
Hai-Tao Yao ◽  
Dong-Pu Liu ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Milk Fat ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Blank Group ◽  
Bovine Mammary Epithelial Cells ◽  
Real Time Quantitative Pcr ◽  
Notable Increase ◽  
Fat Synthesis ◽  
Milk Fat Synthesis

Due to the complexity of insulin in life activities, the role of insulin in mammalian lactation has not been well explained. To investigate the influence of insulin on milk fat synthesis, bovine mammary epithelial cells (BMECs) were cultured in treatment with insulin. We determined the content of Triglyceride (TG) in cell-free culture medium and found a notable increase in TG secrection. Lipid droplet staining study showed a consistent result. We also used real-time quantitative PCR and western blotting to detect the expression of signaling molecules related to milk fat synthesis. We found that insulin resulted in an obvious increase of SREBP-1, mTOR and lipogenic gene expression compared with the blank group. Taken together, our study reveals that insulin plays a significant role in milk fat synthesis.

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