ГІСТОЛОГІЧНИЙ АНАЛІЗ ЛІМФОЇДНИХ УТВОРЕНЬ ШЛУНКА І ТОНКОЇ КИШКИ У КЛІНІКО-ПАТОГЕНЕТИЧНОМУ ПРОФІЛІ БАБЕЗІОЗУ

Peripheral lymphoid organs respond immediately to a decrease in the body's defence responses, and their localisation and morphological specificity account for their active involvement in immunogenesis (especially in animals). This fact accentuates the morpho-functional estimation of the lymphoid tissue condition with the purpose of timely diagnostics of starting deviations and preventing the development of decompensated changes. Thus, the analysis of histological changes of the latter in natural and experimental prototypes of babesiosis is relevant. The aim of the research is to define the role of histological changes of lymphoid masses in the stomach and small intestine in the formation of the clinical and pathogenetic profile of babesiosis. We studied the structural changes of lymphoid masses of the stomach and small intestine in animals with babesiosis postmortem by histological methods. Fixation was traditionally carried out in 12% aqueous formalin solution, dehydration in alcohol (30º to absolute), and pouring (paraffin/ celloidin). Staining was conducted with eosin and haematoxylin, Van Gizon, Brasche. Analysis was performed using an Olympus BX-41 microscope, Japan (x 100; x 200; x 1350). Verification was carried out by cytological, ultramicroscopic, molecular genetic methods (polymerase chain reaction) and in a biological experiment on animals. Analysis of specimens of the lamina propria in the gastric mucosa of the animals in the experimental group and clinical observation group established that the latter contained diffuse accumulations of the lymphoid tissue. Their dispersal involved different parts of the organ, including quite large areas. Haematoxylin and eosin staining gave a clear histological pattern with a marked contrast of fragments, which contributed to diagnostic resources. Lymphoid follicles were observed occasionally, their marginal, mantle zones were blurred. The germinative centres were not detected and did not show any lucency. Neutrophilic granulocytes, monocytes and plasma cells predominated among the cellular leucocyte populations. Examination of the lymphoid apparatus of the small intestine showed predominantly diffuse nodular hyperplasia, which was widespread. A focal version of the nosological prototype was observed in individual specimens occasionally, presented as single cells (most often in the terminal parts of the organ). Histological changes of lymphoid masses of the stomach and small intestine in animals with babesiosis consisted in the development of expressive diffuse nodular hyperplasia with disorganization of the structure of lymphoid follicles, destruction of zonality of the latter, making it impossible to detect germinative centres.

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Immune disorders of the gastrointestinal tract

Oxford Textbook of Medicine ◽

10.1093/med/9780199204854.003.1505_update_001 ◽

2010 ◽

pp. 2244-2256

Author(s):

D.J. Unsworth

Keyword(s):

Small Intestine ◽

Gastrointestinal Tract ◽

B Lymphocytes ◽

Lamina Propria ◽

Thoracic Duct ◽

Lymphoid Tissue ◽

Plasma Cells ◽

Immunoglobulin A ◽

Systemic Circulation ◽

Lymphoid Follicles

The gastrointestinal tract is protected by gut-associated lymphoid tissue that provides an environment where interaction occurs between luminal antigen and specially adapted immune tissue in Peyer’s patches (small intestine only) or lymphoid follicles. T and B lymphocytes primed in the gut migrate into the systemic circulation via the thoracic duct but home preferentially to the lamina propria of the intestine. Plasma cells of the lamina propria secrete immunoglobulin A as a dimer linked by a joining peptide....

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CELLULAR LOCALIZATION OF IMMUNOGLOBULINS WITH DIFFERENT ALLOTYPIC SPECIFICITIES IN RABBIT LYMPHOID TISSUES

Journal of Experimental Medicine ◽

10.1084/jem.122.5.853 ◽

1965 ◽

Vol 122 (5) ◽

pp. 853-876 ◽

Cited By ~ 287

Author(s):

Benvenuto Pernis ◽

Gerolamo Chiappino ◽

Andrew S. Kelus ◽

Philip G. H. Gell

Keyword(s):

Cellular Localization ◽

Plasma Cells ◽

Single Cells ◽

Germinal Centers ◽

The Other ◽

Lymphoid Tissues ◽

Double Staining ◽

Lymphoid Follicles ◽

B Locus ◽

Two Populations

The cellular localization of allotypes in rabbit lymphoid tissues has been studied by immunofluorescence. In heterozygous animals the double staining for two allotypes controlled by allelic genes (A1 and A2; A4 and A5; A4 and A6) has shown the existence of two populations of plasma cells, one containing one allotype and the other the alternative one. The localization in different cells of immunoglobulins marked by allelic allotypic specificities has been confirmed by microspectrography of single cells. An exception to this rule was given by the presence in the germinal centers of lymphoid follicles of apparently uniform mixtures of products of the two allelic genes. Double staining for two allotypes controlled by genes at different loci showed, instead, the presence of many cells containing both allotypes; the number of these cells was highest in doubly homozygotes, in the other it was consistent with random association of non-allelic specificities. In addition double staining for one allotype and gamma G globulins in the lymphoid tissues of rabbits homozygous at the a or at the b locus, has shown the presence of cells containing immunoglobulins that lack one allotype.

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HISTOLOGICAL CHANGES IN THE INTESTINES OF POULTRY DURING FODDER INTOXICATION

STATE AND DEVELOPMENT PROSPECTS OF AGRIBUSINESS. In the frame of the XXIII Agribusiness Forum of the South of Russia and the Exhibition «Interagromash» Volume 1 ◽

10.23947/interagro.2020.1.102-105 ◽

2020 ◽

Author(s):

E.P. Dolgov ◽

◽

A.A. Abramov ◽

E.V. Kuzminova ◽

E.V. Rogaleva ◽

...

Keyword(s):

Body Weight ◽

Histological Examination ◽

Aflatoxin B1 ◽

Structural Changes ◽

Clinical Manifestations ◽

Feed Additives ◽

The Body ◽

Histological Changes ◽

Beet Pulp

The article presents the data on the study of the influence of mycotoxins combination (T-2 toxin at the concentration of 0.095 mg/kg and aflatoxin B1 in the concentration of 0.019 mg/kg) on the body of quails and the results of pharmacocorrection of toxicosis with a complex consisting of beet pulp and lecithin. Structural changes in the intestines of quais at fodder mycotoxicosis are described. The use of antitoxic feed additives in poultry led to a weakening of the action of xenobiotics, which was confirmed by an increase in the safety of poultry and increase in body weight of quails, a decrease in the clinical manifestations of intoxication, as well as in positive changes in the structure of the intestine of the poultry during histological examination.

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HISTOLOGICAL CHANGES IN THE SMALL INTESTINE IN THE ADVANCED STAGES OF BURN DISEASE

World of Medicine and Biology ◽

10.26724/2079-8334-2017-3-61-90-96 ◽

2017 ◽

Vol 13 (61) ◽

pp. 090

Author(s):

G. M. Galunko

Keyword(s):

Small Intestine ◽

Histological Changes ◽

Advanced Stages ◽

Burn Disease

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STRUCTURAL CHANGES IN THE SMALL INTESTINE WALL AT THE MECHANICAL OBSTRUCTION OF THE DIGESTIVE CHANNEL IN THE EXPERIMENT

World of Medicine and Biology ◽

10.26724/2079-8334-2019-1-67-142 ◽

2019 ◽

Vol 15 (67) ◽

pp. 142

Author(s):

I. Ya. Dzyubanovskyi ◽

V. V. Benedykt ◽

S. O. Nesteruk ◽

K. S. Volkov

Keyword(s):

Small Intestine ◽

Structural Changes ◽

Mechanical Obstruction

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Distribution of Laminin Variants and Their Integrin Receptors in Human Secondary Lymphoid Tissue: Colocalization suggests that the α6β4-integrin is a receptor for laminin-5 in lymphoid follicles

Cell Adhesion and Communication ◽

10.3109/15419069609010771 ◽

1996 ◽

Vol 4 (4-5) ◽

pp. 269-279 ◽

Cited By ~ 12

Author(s):

Lies H. Jaspars ◽

Annemieke A. De Melker ◽

Petra Bonnet ◽

Arnoud Sonnenberg ◽

Chris J.L.M. Meijer

Keyword(s):

Lymphoid Tissue ◽

Integrin Receptors ◽

Lymphoid Follicles ◽

Secondary Lymphoid Tissue ◽

Laminin 5 ◽

Α6β4 Integrin

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Morphophysiological studies of the shoot apex. I. Recurrent growth flushes and their relationship with structural changes in the growing apex of Callistemon viminalis

Canadian Journal of Botany ◽

10.1139/b68-171 ◽

1968 ◽

Vol 46 (10) ◽

pp. 1287-1295 ◽

Cited By ~ 7

Author(s):

A. N. Purohit ◽

K. K. Nanda

Keyword(s):

Structural Changes ◽

Rest Period ◽

Growth Cycle ◽

Active Period ◽

Active Growth ◽

Histological Changes ◽

Floral Buds ◽

Rest Periods ◽

Number Of Leaves ◽

Callistemon Viminalis

The annual growth cycle of Callistemon viminalis consists of three phases: (1) a reproductive phase that continues till the end of February; (2) a vegetative phase that lasts from the end of February till the end of August; and (3) a long dormant phase which lasts till the end of December. The cycle exhibits four growth flushes with alternating periods of active growth and rest. The duration of the successive rest periods increases, leading eventually to the onset of the long dormant phase.The growing apex exhibits more or less similar histological changes in different growth flushes. An increase in divisional activity of the cells of flanking meristem, accompanied by a decline in the elongation of cells of pith rib meristem, characterizes the rest period. During the active period of growth, the cells of the pith rib meristem elongate but the activity of the flanking meristem decreases. The number of leaves produced in each growth flush, therefore, is dependent upon the duration of the preceding rest period, and their size and nature on the duration of active growth. In growth flush 1 the cells of the rib meristem show elongation twice during the active period corresponding with the separation of floral buds and of foliage leaves respectively. In the long dormant phase, on the other hand, the cells of the flanking meristem show high divisional activity twice, first during September when new leaf primordia are initiated, and again during November when floral buds are initiated. Events of great significance thus occur during the long dormant phase. The reproductive development is characterized by a marked increase in the rate of cell division in the flanking meristem accompanied by cessation of growth, the features that characterize the rest period as well.

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Inflammation and structural changes of splenic lymphoid tissue in visceral leishmaniasis: A study on naturally infected dogs

Parasite Immunology ◽

10.1111/j.1365-3024.2008.01051.x ◽

2008 ◽

Vol 30 (10) ◽

pp. 515-524 ◽

Cited By ~ 36

Author(s):

C. C. SANTANA ◽

J. VASSALLO ◽

L. A. R. DE FREITAS ◽

G. G. S. OLIVEIRA ◽

L. C. PONTES-DE-CARVALHO ◽

...

Keyword(s):

Visceral Leishmaniasis ◽

Lymphoid Tissue ◽

Structural Changes

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Histochemical Studies on Post Natal Development of Gut Associated Lymphoid Tissue (GALT) of Intestine of Pati Duck (Anas platyrhynchos domesticus) of Assam

Indian Journal of Animal Research ◽

10.18805/ijar.b-4533 ◽

2021 ◽

Author(s):

A. Deka ◽

M. Talukdar ◽

D.J. Talukdar

Keyword(s):

Phosphatase Activity ◽

Lymphoid Tissue ◽

Correct Diagnosis ◽

Peyer's Patches ◽

Peyer’S Patches ◽

Moderate Activity ◽

Strong Reaction ◽

Strong Activity ◽

Lymphoid Follicles ◽

Lymphoid Nodules

Background: The Pati duck constitutes a major indigenous duck variety in the state of Assam. The study of the gut associated lymphoid tissue (GALT) of Pati duck of Assam is of great value in regard to normal academic and bio-medical research aspects. It is also pre-requisite for correct diagnosis and evaluating the treatment of certain diseases like duck virus enteritis, duck cholera, aflatoxicosis, botulism etc, caused by different types of pathogens, food poisoning and food allergy. Methods: The study was conducted on Pati duck of Assam in where 45 numbers of ducks were selected by irrespective of sex at different stages of development. The birds were divided into five groups depending on its age viz., 1st week, 4th week, 16th week, 24th week and 42nd weeks old. The pieces of gut having lymphoid tissue or Peyer’s patches were collected immediately after slaughter. Samples were made cryosections (-20oC) at 10µm in thickness and were temporally stored at (-22oC). The histochemical staining was done after that. Result: The acid phosphatase activity was weak in the lymphoid follicles of 1st and 4th week of age of Pati duck, while its activity was moderate in 16th, 24th and 42th week of age.The alkaline phosphatase activity was moderate reaction in the lymphoid follicles of 1st and 4th week of age of Pati duck, while its activity was intense in 16th, 24th and 42th week of age of duck. The lymphoid follicles of both Peyer’s patches and solitary lymphoid nodules were showed moderate activity for adenosine tri-phosphatase activity in 1st and 4th week old Pati duck and strong activity in 16th, 24th and 42th week of age of Pati duck, respectively. The lymphoid nodules of intestine showed strong reaction for non-specific esterase activity in all the age groups of Pati duck.

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