Recombinant analogue of the human protein SLURP-1 inhibits the growth of multicellular carcinoma cell spheroids

2019 ◽  
Vol 489 (5) ◽  
pp. 517-520
Author(s):  
M. L. Bychkov ◽  
M. A. Shulepko ◽  
O. V. Shlepova ◽  
E. N. Lyukmanova ◽  
M. P. Kirpichnikov
Keyword(s):  
3D Model ◽  
Human Lung ◽  
A549 Cells ◽  
Growth Factor Receptor ◽  
Multicellular Spheroids ◽  
A431 Cells ◽  
Combined Application ◽  
Epidermal Growth ◽  
Lung Adenocarcinoma A549 ◽  
Human Epidermoid Carcinoma

The present study showed that the recombinant analogue of SLURP‑1 effectively inhibits the growth of a 3D model of tumors - multicellular spheroids from human epidermoid carcinoma A431 cells and human lung adenocarcinoma A549 cells. The combined application of SLURP‑1 with gefitinib (inhibitor of epidermal growth factor receptor (EGFR)) leads to the synergistic antiproliferative effect on spheroids from A431 cells. The results obtained suggest the possibility for design of first-in-class anticancer drugs based on recombinant SLURP‑1.

scholarly journals Shikonin causes cell-cycle arrest and induces apoptosis by regulating the EGFR–NF-κB signalling pathway in human epidermoid carcinoma A431 cells

2015 ◽  
Vol 35 (2) ◽  
Author(s):  
Rong Tian ◽  
You Li ◽  
Mei Gao
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Growth Factor Receptor ◽  
Signalling Pathways ◽  
Nuclear Factor ◽  
A431 Cells ◽  
Cycle Arrest ◽  
Epidermal Growth ◽  
Light Chain Enhancer ◽  
Human Epidermoid Carcinoma

Our findings indicated that shikonin-inhibited cell growth and caused cell-cycle arrest of the A431 cells through the regulation of apoptosis. Moreover, these effects were mediated, at least partially, by suppressing the activation of the EGFR (epidermal growth factor receptor)-NF-κB (nuclear factor kappa-light-chain-enhancer of activated B-cells) signalling pathways.

scholarly journals The Cytotoxicity of RNase-Derived Peptides

Biomolecules ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 16
Author(s):  
Vera Ulyanova ◽  
Elena Dudkina ◽  
Alsu Nadyrova ◽  
Vladimir Kalashnikov ◽  
Yulia Surchenko ◽  
...  
Keyword(s):  
Tumor Targeting ◽  
Cytotoxic Effect ◽  
Egf Receptor ◽  
Human Lung ◽  
A549 Cells ◽  
Cytotoxic Potential ◽  
Epidermal Growth ◽  
Structural Parts ◽  
Lung Adenocarcinoma A549 ◽  
Mcf 7

Bacterial ribonuclease binase exhibits a cytotoxic effect on tumor cells possessing certain oncogenes. The aim of this study was to identify the structural parts of the binase molecule that exert cytotoxicity. Out of five designed peptides, the peptides representing the binase regions 21–50 and 74–94 have the highest cytotoxic potential toward human cervical HeLa and breast BT-20 and MCF-7 cancer cells. The peptides B21–50 and B74–94 were not able to enter human lung adenocarcinoma A549 cells, unlike BT-20 cells, explaining their failure to inhibit A549 cell proliferation. The peptide B74–94 shares similarities with epidermal growth factor (EGF), suggesting the peptide’s specificity for EGF receptor overexpressed in BT-20 cells. Thus, the binase-derived peptides have the potential of being further developed as tumor-targeting peptides.

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