scholarly journals Activity of enzymes of tyrosine metabolism in the rat liver under the conditions of acetaminophen-induced hepatitis on the background of protein deficiency

2020 ◽  
Vol 12 (1) ◽  
pp. 14-19
Author(s):  
Oksana Voloshchuk ◽  
Halyna Kopylchuk

The contribution of the mis-metabolism of individual amino acids to the development of drug-induced damage to liver cells remains unexplored. The aim of the present study was to investigate the changes in liver tyrosine level and activity of the enzymes of its metabolism: tyrosine aminotransferase, 4-hydroxyphenylpyruvate dioxygenase, aldehyde dehydrogenase ALDH3A1 under the conditions of acetaminophen-induced hepatitis on the background of protein deficiency. Determination of tyrosine in deproteinized with 6% sulfosalicylic acid extracts of the liver tissue was performed using the automatic analyzer of amino acids T-339 (“Microtechnology”, Czech Republic). The enzyme activity was determined by spectrophotometric method – tyrosine aminotransferase by the amount of 4-hydroxybenzaldehyde, which has a maximum absorption at 330 nm, 4-hydroxyphenylpyruvate dioxygenase – by the colored product intensity at λ 336 nm, aldehyde dehydrogenase ALDH3A1 activity was measured at 340 nm wavelength. Results have shown that in animals with toxic liver injury which were maintained in conditions of alimentary protein deficiency, a 5-fold decrease in tyrosine level in the liver was observed. At the same time in animals of this group there was a decrease in TAT activity by 1.6 times, a 4-fold decrease in activity of aldehyde dehydrogenase ALDH3A1 and increase in the activity of 4-hydroxyphenylpyruvate dioxygenase by 2.5 time comparing to control parameters. Conclusion was made, that alimentary protein deficiency is a factor leading to an intensification of tyrosine metabolism disturbances in animals with toxic liver injury. The pronounced exhaustion of the tyrosine pool is accompanied by the activation of the homogentisate pathway of its metabolism, as evidenced by the increase in the activity of 4-hydroxyphenylpyruvate dioxygenase and simultaneous reduction in the aldehyde dehydrogenase ALDH3A1activity. The established changes open prospects to study the possible targets for the exogenous correction of metabolic disorders under the conditions of intoxication with acetaminophen, especially in people with protein deficiency.

1990 ◽  
Vol 10 (3) ◽  
pp. 371-379 ◽  
Author(s):  
Sally A. Weisdorf ◽  
Deborah K. Freese ◽  
William J. Radmer ◽  
Louis P. Dehner ◽  
Frank B. Cerra

2021 ◽  
Author(s):  
Sarah Ordway ◽  
Brett Sadowski ◽  
Kathryn E Driggers ◽  
Ryan Kwok

ABSTRACT Objectives Drug-induced liver injury (DILI) is a significant cause of morbidity and mortality. Establishing a diagnosis is challenging due to the broad differential diagnosis of liver injury. We retrospectively reviewed patients with severe idiosyncratic DILI at Walter Reed National Military Medical Center in order to define the scope and patterns of injury in the military population. Methods Using the military health database, we identified a total of 110 patients who had an International Classification of Disease (ICD)-10 code for toxic liver injury in the electronic medical record at Walter Reed National Military Medical Center between 2016 and 2019. Each patient record was reviewed, and all pertinent data for included patients were recorded into a database for analysis. Results Twenty-seven out of 110 patients with a diagnostic code for toxic liver injury met inclusion criteria for severe idiosyncratic DILI. Nine cases were caused by supplements, including 5 active duty service members using synthetic anabolic steroids or preworkout supplements. The majority of patients were men and one-third were serving on active duty. The ranges of liver enzyme elevation and patterns of liver injury widely varied. Conclusion Military service members are at particularly high risk for DILI given the frequent use of over-the-counter and other unregulated strength- and performance-enhancing supplements. These injuries not only have significant medical consequences but can profoundly impact military readiness and mission capability. Diagnosis of DILI among active duty service members requires a strong index of suspicion, and inquiry regarding all ingestions is crucial. Educating physicians, providers, and policy makers on the risks of supplement-induced liver injury among service members is crucial. These data will facilitate additional studies exploring susceptibility to severe idiosyncratic DILI among the military population.


2010 ◽  
pp. 189-190
Author(s):  
Henryk Dancygier ◽  
Peter Schirmacher

1964 ◽  
Vol 46 (4) ◽  
pp. 424-433 ◽  
Author(s):  
Kurt J. Isselbacher ◽  
Wallace A. Jones

Praxis ◽  
2010 ◽  
Vol 99 (21) ◽  
pp. 1259-1265 ◽  
Author(s):  
Bruggisser ◽  
Terraciano ◽  
Rätz Bravo ◽  
Haschke

Ein 71-jähriger Patient stellt sich mit Epistaxis und ikterischen Skleren auf der Notfallstation vor. Der Patient steht unter einer Therapie mit Phenprocoumon, Atorvastatin und Perindopril. Anamnestisch besteht ein langjähriger Alkoholabusus. Laborchemisch werden massiv erhöhte Leberwerte (ALAT, Bilirubin) gesehen. Der INR ist unter oraler Antikoagulation und bei akuter Leberinsuffizienz >12. Die weiterführenden Abklärungen schliessen eine Virushepatitis und eine Autoimmunhepatitis aus. Nachdem eine Leberbiopsie durchgeführt werden kann, wird eine medikamentös-toxische Hepatitis, ausgelöst durch die Komedikation von Atorvastatin, Phenprocoumon und Perindopril bei durch Alkohol bereits vorgeschädigter Leber diagnostiziert. Epidemiologie, Pathophysiologie und Klink der medikamentös induzierten Leberschäden (drug induced liver injury, DILI), speziell von Coumarinen, Statinen und ACE-Hemmern werden im Anschluss an den Fallbericht diskutiert.


Hepatology ◽  
2004 ◽  
Vol 40 (4) ◽  
pp. 773-773 ◽  
Author(s):  
Jay H. Hoofnagle

2011 ◽  
Vol 49 (08) ◽  
Author(s):  
C Agne ◽  
K Rifai ◽  
HH Kreipe ◽  
MP Manns ◽  
F Puls

2015 ◽  
Vol 53 (12) ◽  
Author(s):  
AB Widera ◽  
L Pütter ◽  
S Leserer ◽  
G Campos ◽  
K Rochlitz ◽  
...  

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