scholarly journals PROPAGASI IN VITRO ANGGREK (Dendrobium phalaenopsis Fitzg) TERHADAP PEMBERIAN HORMON IBA DAN KINETIN

2017 ◽  
Vol 7 (1) ◽  
pp. 15
Author(s):  
IMAM MAHADI
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Growth Regulator ◽  
Plant Growth Regulator ◽  
Ornamental Plants ◽  
Special Treatment ◽  
Culture Techniques ◽  
Tissue Culture Techniques ◽  
Number Of Shoots

Orchids are a very popular ornamental plants, but orchids have a very slow vegetative growth, thus requiring special treatment in the grown.To  spur grow than development can be used through tissue culture techniques of multiplication. But the success of the technique of tissue culture is inseparable with give a substance on the plant growth regulator. This study aims to determine the growth  an influence  D.phalaenopsis Fitzg orchids of giving a gaint swith IBA administration and Kinetin according to in vitro  and as well as to obtain a concentration combination of  IBA and Kinetin on the best orchid D.Phalaenops is Fitzg explants.  Base don’t he results of the study indicate that the combination of IBA and Kinetin concentration giving a influence percentage of live explants,   number of shoots and number of roots orchid D. phalaenopsis Fitzg explants.Combination treatment of IBA 1.0 ppm and 0.1ppm Kinetin is a combination of the best treatment to produce a number of shoots orchids.The giving of IBA 0.5ppm in the medium without the addition of kinetin concentration can increase the number of roots of the orchid D.phalaenopsis Fitzg.

scholarly journals Pertumbuhan dan Perkembangan Protocorn Like Bodies (PLB) Anggrek Papua Dendrobium discolor dengan Perlakuan Auksin dan Sitokinin

2021 ◽  
Vol 13 (1) ◽  
pp. 28-35
Author(s):  
Agustriani Warpur ◽  
Linus Y. Chrystomo ◽  
Suharno Suharno
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Combination Treatment ◽  
Growth Regulator ◽  
Plant Growth Regulator ◽  
Leaf Length ◽  
Significant Treatment ◽  
Culture Techniques ◽  
Growth Regulator Treatment

Dendrobium could meet the demands of domestic and foreign consumers from time to time because of their very charming flower blossoms. Orchid flowers are attractive because of the variety their color, size, shape, composition, number of buds the blossom of flowers, especially for the color it is vary greatly. One of the potential Dendrobium Papua orchids is Dendrobium discolor. To fulfill the needs of Dendrobium orchids in pots or in peaces it is depend on the success of Protocorn Like Bodies (PLB) cultivation. The purpose of this study was to determine the growth and development of PLB cultivated in vitro on Vacint & Went (V&W) base media with a combination of different plant growth regulators. The method used wasPLB cultivation with tissue culture techniques in vitro on V&W media with a factorial completely randomized design (CRD). The first factor was auxin treatment (2,4D) with 5 concentrations 1, 2, 3, and 4 ppm plus control (without plant growth regulator treatment), while the second factor was cytokinin treatment with 5 concentrations 1, 2, 3, and 4 ppm plus 1 control (without plant growth regulator treatment). Each treatment repeated 5 times, threfore, there are 125 treatment units in total 125 treatment units. The media used were Vacin and Went (VW). The variables measured were leaf length and the number of leaves per plantlet in each combination treatment of plant growth regulator (PGR) after one month of cultivation. Data were analyzed by using variance (ANOVA) to determine the significance. The results of this study indicated that the combination treatment of auxin and cytokinin PGR in V&W media had a significant effect on the length and number of plantlets leaves and the most significant treatment was the combination of PGR auxin: cytokinin = 0:3 ppm. So it can be concluded that the best medium for PLB culture is a combination of PGR auxin: cytokinin = 0:3 ppm. with an average value of 3.5 cm plantlet leaf length and number of 6 leaves per plantlet. Key words: Protocorn Like Bodies; tissue culture; V&W medium; auxins; cytokinins.

DEVELOPMENT OF NEOLAMARCKIA CADAMBA (KELEMPAYAN) TISSUE CULTURE TECHNIQUES FOR SUSTAINABLE SUPPLY OF PLANTING MATERIALS FOR COMMERCIAL PLANTATION

2015 ◽  
Vol 77 (24) ◽  
Author(s):  
Siti Suhaila A. Rahman ◽  
Norwati Muhammad ◽  
Nor Hasnida Hassan ◽  
Haliza Ismail ◽  
Nazirah Abdullah ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Mass Production ◽  
Nodal Segments ◽  
Direct Organogenesis ◽  
Timber Species ◽  
Culture Techniques ◽  
Commercial Plantation ◽  
Planting Materials ◽  
Tissue Culture Techniques

Neolamarckia cadamba (kelempayan) is a multipurpose and fast growing timber species. The tree is grown for timber, paper-making and as ornamental plant. It is reported that its barks and leaves possesed medicinal values and its flowers are used in perfumes. The species is also known to be suitable for plywood, packing case, toys and short-fibred pulp. Therefore, mass production of high quality planting material of N. cadamba is important to support plantation program of this species. Here we presented mass production of N. cadamba through tissue culture techniques. Nodal segments derived from in vitro germinated seeds were used and induced direct organogenesis to produce shoots and roots using MS media (1962) and plant growth regulators (BAP and IBA) that are relatively cheaper than previously used methods. The tissue culture technique of N. cadamba developed may help in ensuring supply of planting materials that are feasible for commercial plantation purposes.

scholarly journals The effect of NAA and coconut water combination on garlic (Allium sativum L.) tissue culture

2021 ◽  
Vol 905 (1) ◽  
pp. 012036
Author(s):  
Nandariyah ◽  
L S Mahmudah ◽  
R B Arniputri ◽  
A T Sakya
Keyword(s):  
Tissue Culture ◽  
Coconut Water ◽  
Emergence Time ◽  
Shoot Height ◽  
Culture Techniques ◽  
Randomized Design ◽  
Two Factors ◽  
Number Of Leaves ◽  
Tissue Culture Techniques ◽  
Number Of Shoots

Abstract Tissue culture techniques can increase the number of garlic seedlings. The purpose of this research is to determine the effect of NAA and coconut water in increasing the number of garlic seeds. This research used a Completely Randomized Design of two factors. The treatment used is NAA with concentrations of 0 ppm, 0.5 ppm, 1 ppm, 1.5 ppm, and coconut water concentrations of 0%, 10%, 20%. The variables observed were shoot emergence time, root emergence time, number of shoots, number of roots, number of leaves, shoot height, root length, and number of plantlets. The results showed that the addition of coconut water 20% without the addition of NAA in 1 bulb can produce 3.33 planlets and the results of explant propagation in 1 bulb can produce the number of shoots as many as 15.33 shoots. Giving coconut water with concentrations of 10% and 20% can increase the number of leaves, shoot height, and some planlets. The concentration of NAA 0.5 ppm can accelerate the root emergence time on garlic explant.

Effects of Different Plant Growth Regulator on in vitro Shoot Multiplication of Chrysanthemum morifolium

2003 ◽  
Vol 3 (6) ◽  
pp. 553-560 ◽  
Author(s):  
M.Z. Karim . ◽  
M.N. Amin . ◽  
M.A.K. Azad . ◽  
F. Begum . ◽  
M.M. Rahman . ◽  
...  
Keyword(s):  
Plant Growth ◽  
Growth Regulator ◽  
Plant Growth Regulator ◽  
Shoot Multiplication ◽  
Chrysanthemum Morifolium

Influence of plant growth regulator upon in vitro propagation of Paulownia sp

2015 ◽  
Vol 208 ◽  
pp. S113
Author(s):  
Marcel Danci ◽  
Ioana Cretu ◽  
Giancarla Velicevici ◽  
Roxana Luca ◽  
Alina Simina ◽  
...  
Keyword(s):  
Plant Growth ◽  
Growth Regulator ◽  
Plant Growth Regulator ◽  
In Vitro Propagation

scholarly journals In vitro plantlet production of the endangered Pinguicula vulgaris

2012 ◽  
Vol 7 (1) ◽  
pp. 48-53 ◽  
Author(s):  
Tomás Grevenstuk ◽  
Anabela Romano
Keyword(s):  
Plant Growth ◽  
Growth Regulator ◽  
Plant Growth Regulator ◽  
Normal Development ◽  
Shoot Proliferation ◽  
Carnivorous Plant ◽  
Habitat Requirements ◽  
Ex Vitro ◽  
Plantlet Production

AbstractThis study describes the development of a micropropagation protocol for Pinguicula vulgaris using cultures initiated from in vitro produced seedlings. P. vulgaris is a carnivorous plant with a northern, disjunctly circumpolar distribution and specific habitat requirements, and is hence becoming increasingly rare. Shoot proliferation was significantly influenced by Murashige and Skoog (MS) macronutrient concentration, showing higher proliferation rates in 1/4MS, but was not affected by the addition of 0.1 mg/L 6-benzyladenine (BA) or zeatin (Zea). The best medium for propagating P. vulgaris was plant growth regulator (PGR) free ¼MS. An average of 7.62 new shoots per initial explant could be obtained after 8 weeks of culture, of which over 79% produced roots during proliferation. Moreover, rooting percentages of 100% were obtained for the initial explants in all the tested media, including media without PGRs. The plantlets were successfully acclimatized to ex vitro conditions, exhibiting normal development.

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