Objective: To study the prevalence of 25-hydroxyvitamin D deficiency pattern during three year (2017-2020) and severity correlation among individuals with acute traumatic brain injury (TBI). Methodology: Subjects with acute TBI admitted from June 1st, 2017 through June 30th, 2020 were recruited. 280 out of 445 met inclusion criteria. The demographic injury related details, assessment of 25 OH vitamin D and Glasgow Comma (GCS) score were done at the time of admission. Results: The year wise enrolled subjects were young with mean age of 28.39±0.86 years with males (73.3%) and female (23.7%), in first year, 27.77±5.35 years with males (81.67%) and female (18.33%), in second year and 23.04±7.10 years with males (88.57%) and female (11.42%), in third years. Mean value of 25(OH) vitamin D in subjects during three years were 23.78±11.79ng/mL, 21.65±12.53 ng/mL and 25.18±18.58ng/mL. The vitamin D deficiency levels in this study were tabulated as: deficient (level <20 ng/mL), insufficient (level 20–29.9ng/mL), and sufficient (level ≥30ng/mL). Which were found during three years as: In First year, Deficient (64.44%), Sufficient (11.11%), insufficient (24.44%), in second years, Deficient (88.33%), Sufficient (2.66%), insufficient (10.00%) and in third year Deficient (88.57%), Sufficient (1.42%), insufficient (10.00%). In which sufficient level were found to be decreased statistically significant with years with P value= 0.0001. The severity assessment through GCS score were found to be statistically increased with deficient levels with P values=0.0447, but found no significance, when comparison were done between years wise GCS score and levels of vitamin D. Conclusion: The study found decreased prevalence of vitamin D deficiency levels with increased severity. Therefore it should be routinely screened and treated as indicated.