Health anxiety will increase as a result of COVID-19. Many people who feel vulnerable, including individuals with disabilities and chronic illnesses, may be more susceptible to stigmatize individuals who have an infection. Researchers in this study aimed to develop a measure of negative attitudes toward COVID-19 among people with disabilities and chronic illnesses to better understand how stigmatization, which can cause negative outcomes, plays a role in individuals with disabilities' response to COVID-19. The study was conducted with 269 participants with any disability or chronic illness in which all completed the online questionnaire. To examine the psychometric properties of the COVID-19 Negative Attitudes Scale (COVID-19 NAS), an exploratory factor analyses, confirmatory factor analyses, reliability analysis, and correlation were conducted. In addition, concurrent validity was assessed through correlations with participants' stress related to COVID-19, depression and anxiety, and resilience. Exploratory factor analyses demonstrated that COVID-19 NAS is a unidimensional scale. Confirmatory factor analysis results confirmed the one dimensionality of the scale. Our findings indicate that the COVID-19 NAS could be a valuable tool in assessing negative attitudes toward COVID-19 for people with chronic conditions and disabilities.