scholarly journals Degradation of Malachite Green by Green Synthesized Copper Nanoparticles by Using Aloe Barbadensis Leaf Extracts

Author(s):  
Madiha Batool
Author(s):  
Manmeet Kaur ◽  
Suman Prajapati ◽  
Samneek Cholia ◽  
Jaskeet Singh Mann ◽  
Gurpreet Singh

Background: In the recent years, the green synthesis of nanoparticles has taken a lead role over the conventional chemical and physical approach due to its non-toxic, cost effective parameters and has found its place in various applications. Objectives: The major objectives of this study was to synthesise and characterize the copper nanoparticles using the rose extract at different set of conditions and analyse these nanoparticles as a source of dye degradation agent under sunlight conditions. Methods: Present study was conducted with the aim to synthesis the copper nanoparticle using the rose petal extract. The components present the in the extract act as the reduction and stabilization agents for the synthesis of CuNPs. The synthesized nanoparticles were characterized by using UV-VIS, FTIR, XRD and SEM analysis. Photocatalytic degradation of two dyes (Malachite Green and Carbol fuchsin) was analysed using double beam spectroscopic analysis Results: UV-Vis analysis indicated the presence of a peak at around 630 nm. The FT-IR analysis indicated the involvement of various biomolecules during the synthesis of nanoparticles. The structure and the conformation was elucidated using XRD and SEM showed the agglomerated form of the synthesized nanoparticles with the size range of about 60-90 nm. The synthesised copper nanoparticles was used for degradation of malachite green and carbol fuchsin dye using photocatalytic under sunlight irradiation. UV-Vis spectral analysis indicated that synthesised copper nanoparticle act more effective in degradation of malachite green (around 95%) whereas carbol fuchsin showed a maximum degradation by 52% therefore suggesting that CuNPs act as an efficient photo catalyst in dye degradation. Conclusion: The results obtained from this study indicates that rose extract has the potential of synthesis of copper nanoparticles which is non-toxic and convenient approach as compared to physical and chemical synthesis. These nanoparticles can be effectively employed as dye decolourization agents to treat industrial effluent and prevent the environmental pollution.


2015 ◽  
Vol 832 ◽  
pp. 132-138
Author(s):  
E. Thanikaivelan ◽  
R. Jothilakshmi ◽  
P. Murugakoothan

The copper nanoparticles were synthesized by green technology using leaf extracts of Ocimum sanctum. The synthesized copper nanoparticles were confirmed by the change of colour after the addition of leaf extract into the copper sulfate solution. The synthesized copper was characterized by X-ray Diffraction (XRD), transmission electron microscopy (TEM) and UV-vis absorption spectroscopy. The copper nanoparticles are crystallized with FCC structure. The synthesized copper nanoparticles exhibit spherical morphology with average particle size of 20 nm. The copper nanoparticle exhibits absorption broad band between 550 nm – 575 nm. The optical trapping effect of Gaussian beam acting on a copper nanoparticle in Rayleigh regime was studied. The optical scattering and optical gradient forces were calculated for 20 nm copper particle.


2021 ◽  
Author(s):  
Roberta L. C. Dayrell ◽  
Gregory R. Cawthray ◽  
Hans Lambers ◽  
Kosala Ranathunge

Abstract Aim Organic substances in leaves of several southwest Australian native species interfere with sensitive colorimetric assays and prevent quantification of inorganic phosphate concentration ([Pi]). We aimed to develop a reproducible routine procedure for treating leaf extracts with activated charcoal (AC) to remove interfering substances, allowing the determination of [Pi] by the malachite green spectrophotometric assay. Methods Leaf extracts of native plants from southwest Australia in 1% (v/v) acetic acid were treated with 10 mg mL−1 acid-washed AC for removal of interfering substances. Standard solutions (0 to 18 μM Pi) with and without AC treatment were compared to quantify Pi loss. A spiking and recovery test was performed to validate the AC treatment. Results Leaf extracts treated with AC exhibited distinguishable absorbance peaks for the malachite green-orthophosphate complex between 630 and 650 nm, as opposed to untreated samples. The Pi-adsorption by AC represented a relatively larger fraction of [Pi] in solutions at 0–4 μM Pi range and stabilised at higher [Pi] when maximum adsorption capacity of AC reached at 11.7 μg Pi g−1AC. The Pi recovery after AC treatment in spiked samples ranged between 100 and 111%. Conclusion The AC treatment successfully removed interfering substances from samples but caused Pi loss. Thus, quantification of [Pi] in AC-treated extracts requires sample [Pi] ≥ 6 μM Pi and the use of AC-treated standards. The error of the AC treatment was minor compared with environmental variability of leaf [Pi]. The AC treatment was a reproducible time- and cost-effective method to remove interfering substances from leaf extracts.


Author(s):  
Archana Rai ◽  

Natural products are valuable and well known for their biological activities. In the current scenario, the research and analysis of plant leaf extract and nanoparticles synthesis with their biological activities has been expended significantly. For the synthesis of nanoparticles, Copper is the preferred metal among other metals due to its reported use in medical field as antimicrobial agents and its lethality. The aim of the present work is to assess antimicrobial and antioxidant activities of the methanolic leaf extracts and aqueous leaf extract mediated copper nanoparticles of Parthenium hysterophorus. Study also revealed the comparative analysis between methanolic leaf extracts and copper nanoparticles. Synthesis of copper nanoparticles (CuNPs) was confirmed by the change of color of aqueous extract, which were further confirmed by using UV-Vis spectrophotometer. The results indicated that copper nanoparticles have great antimicrobial activity against Bacillus subtilis, Staphylococcus aureus, Candida albicans and Aspergillus flavus rather than methanolic leaf extract. These studies confirmed that copper nanoparticles are capable to rendering high antimicrobial efficacy and hence has a great potential in the development of antimicrobial agents. Based on the DPPH, copper nanoparticles found to be good antioxidant when compared to methanolic leaf extract. Furthermore cells viability assay was also done against copper nanoparticles. These results concluded that copper nanoparticles are good source of therapeutic agent and applications of copper nanoparticles based on these findings may lead to valuable discoveries in various fields such as medical devices and antimicrobial systems.


2021 ◽  
Vol 6 (1) ◽  
pp. 52-67
Author(s):  
Saurabh Sharma ◽  
Kuldeep Kumar ◽  
Naveen Thakur

Abstract The presence of various phytochemicals makes the leaf extract-based green synthesis advantageous to other conventional methods, as it facilitates the production of non-toxic by-product. In the present study, leaf extracts from two different plants: Aloe barbadensis miller and Ocimum tenuiflorum, were used to synthesise Ag nanoparticles. The absorbance at 419-432 nm from UV-visible spectroscopy indicates the formation of Ag in the synthesised samples. The effect of precursors’ concentration on the stability, size and shape of the synthesised samples has also been investigated at constant heating temperature, stirring time, and the pH of the solution. The TEM results showed that all the synthesised samples of nanoparticles demonstrated stability with a size range of 7-70 and 9-48 nm with Aloe barbadensis miller and Ocimum tenuiflorum leaf extracts, respectively. The formation of smaller Ag nanoparticles due to utilisation of different precursor concentration and leaf extracts was also explained. The synthesised samples’ anti-bacterial activity was examined against the pathogens, Bacillus subtilis, Staphylococcus aureus, and Escherichia coli. In general, the green synthesis approach established a prospective for developing highly stable Ag nanoparticles with rigid particle shape/size distribution from different leaf extracts for the development of better anti-bacterial agents.


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