scholarly journals INVESTIGATING COOLING RATE AND FETAL BOVINE SERUM CONCENTRATION ON SURVIVAL AND STEMNESS OF MESENCHYMAL STEM CELLS AFTER CRYOPRESERVATION

2009 ◽  
Vol 12 (9) ◽  
pp. 12-22
Author(s):  
Phuc Van Pham ◽  
Tam Thanh Nguyen ◽  
Nhung Thi Hong Vuong ◽  
Tuyet Thi Bach Duong ◽  
Ngoc Kim Phan
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Serum Concentration ◽  
Cord Blood ◽  
Umbilical Cord Blood ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Fetal Bovine Serum Concentration ◽  
Fetal Bovine ◽  
Different Sources

Mesenchymal stem cells (MSCs) can be derived from many different sources. Umbilical cord blood is a rich source of MSCs. The cryopreservation of MSCs that MSCs are still alive and differentiate into many different kinds of functional cells is very important. The aims of this research are to identify ratio of alive and dead cells as well as stemness of them after thaw. The results showed that the stemness was not affected by cryopreservative protocols or media. All cells being alive after thaw could form colonies and differentiate into adipocytes and osteoblasts. Ratio of alive and dead cells was affected very much by cryopreservative protocols and media.

scholarly journals Cord blood serum harvesting by hydroxyethyl starch: a fetal bovine serum alternative in expansion of umbilical cord-derived mesenchymal stem cells

2020 ◽  
Vol 72 (4) ◽  
pp. 551-567
Author(s):  
Maryam Samareh Salavati Pour ◽  
Reza Vahidi ◽  
Mahla Lashkari ◽  
Ali Derakhshani ◽  
Zahra Ameri ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Blood Serum ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Hydroxyethyl Starch ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Cord Blood Serum ◽  
Fetal Bovine

scholarly journals Human Umbilical Cord Blood Serum Has Higher Potential in Inducing Proliferation of Fibroblast than Fetal Bovine Serum

2017 ◽  
Vol 1 (2) ◽  
pp. 65 ◽  
Author(s):  
Ferry Sandra ◽  
Rita Lahirin
Keyword(s):  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Umbilical Vein ◽  
Human Umbilical Cord Blood ◽  
Human Umbilical Cord ◽  
Nih3t3 Cells ◽  
Fetal Bovine

Background: Cytokines and growth factors were reported to play an important role in stimulating fibroblast proliferation. In vitro culture, fibroblast is mostly culture in medium containing fetal bovine serum (FBS).  Human umbilical cord blood (hUCB) has been reported to have low immunogenic property and potential in wound healing, so therefore hUCB serum (hUCBS) could be potential and were investigated in current study.Materials and Methods: Five hUCBs were collected from healthy volunteers with normal delivering procedure. hUCB was ex utero immediately collected from umbilical vein in vacutainers and processed. NIH3T3 cells were cultured in DMEM with 10% FBS or 5-20% hUCBS for 48 hours. Cells were then quantified using MTT assay. Protein concentration of FBS and hUCBS were quantified using Bradford assay.Results: NIH3T3 cells density grown in DMEM with 10% FBS was the lowest. NIH3T3 cells densities were increased along with the increment of hUCBS concentrations. MTT results showed that average number of NIH3T3 cells grown in DMEM with 10% FBS was 6,185±1,243. Meanwhile average numbers of NIH3T3 cells grown in DMEM with 5%, 10% and 20% hUCBS were 8,126±628, 9,685±313 and 12,200±304, respectively. Average numbers of NIH3T3 cells grown in DMEM with 5% hUCBS were significantly higher than the ones with 10% FBS (p=0.000). Bradford results showed that concentration of hUCBS was significantly higher than the one of FBS (p=0.000).Conclusion: hUCBS could induce higher proliferation rate of NIH3T3 cells than FBS. Hence hUCBS could be suggested as an alternate of FBS in inducing fibroblast.Keywords: NIH3T3, fibroblast, UCB, serum, FBS, proliferation

Sign in / Sign up

Export Citation Format

Share Document