scholarly journals Micropropagation and nursery at garden of Dendrobium caesar

2019 ◽  
Vol 2 (3) ◽  
pp. 14-22
Author(s):  
Quang Minh Pham ◽  
Kien Cong Duong ◽  
Phuong Ngo Diem Quach ◽  
Minh Thi Thanh Hoang
Keyword(s):  
Tissue Culture ◽  
Sodium Hypochlorite ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Growth And Development ◽  
Active Charcoal ◽  
Established Method ◽  
Coconut Husk ◽  
In Vitro Plant

Dendrobium is one of the largest and most important orchid genera because of their ornamental and commercial value. Plant tissue culture is an established method for the effective micropropagation of of valuable plants. In this study, Dendrobium caesar keikis were sterilized with sodium hypochlorite 2.5 % in 15 minutes. Effects of 3 kinds of cytokinin (TDZ, BA and kinetin), adenine and 3 kinds of auxin (IAA, IBA, NAA) with different concentrations on inducing shoots and roots were studied. The highest shoot initation was observed on Knudson C (KC) medium supplemented with BA 2.0 mg/L. Strong roots were induced in KC media supplied with 0.5 g/L active charcoal (AC) and 1.0 mg/L IAA. In order to transfer in vitro plant to the nursery garden, the highest percentage of survival seedling was shown on coconut husk substrate, and peanut husk was the best for the growth and development of Dendrobium caesar. Micropropagation and nursery at garden for Dendrobium caesar were successfully established.

Media derived from brown seaweeds Cystoseira myriophylloides and Fucus spiralis for in vitro plant tissue culture

2016 ◽  
Vol 128 (2) ◽  
pp. 437-446 ◽  
Author(s):  
Siham Esserti ◽  
Mohamed Faize ◽  
Lalla Aicha Rifai ◽  
Amal Smaili ◽  
Malika Belfaiza ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Brown Seaweeds ◽  
Fucus Spiralis ◽  
In Vitro Plant

scholarly journals In vitro plant tissue culture: means for production of biological active compounds

Planta ◽  
2018 ◽  
Vol 248 (1) ◽  
pp. 1-18 ◽  
Author(s):  
Claudia A. Espinosa-Leal ◽  
César A. Puente-Garza ◽  
Silverio García-Lara
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Active Compounds ◽  
In Vitro Plant

scholarly journals Effect of Banana Extract on Growth and Development of Protocorm Like Bodies in Dendrobium sp. orchid

2016 ◽  
Vol 13 (1) ◽  
pp. 101-108 ◽  
Author(s):  
M Obaidul Islam ◽  
Md. Serazul Islam ◽  
Md Abu Saleh
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Growth And Development ◽  
Basal Medium ◽  
Plantlet Regeneration ◽  
Nutrient Requirement ◽  
Effect On Growth

An experiment was carried out in Plant Tissue Culture Laboratory, Department of Crop Botany, Bangladesh Agricultural University, Mymensingh to investigate the effect of banana extract on micropropagation of  <i>Dendrobium</i> sp. var. Sonia orchid through PLBs. The experiment was conducted during July 2012 to October 2013. Half-Murashige and Skoog (1/2MS) medium were used as basal medium and the medium was supplemented with banana extract at 12.5, 25, 50, 100, and 200 ml L-1 with a control, where no banana extract was supplemented. The cultures were done in 100 ml conical flasks and maintain at 25°C with 30µ mol m-2 S-1 lighting provided by florescent tubes for 16 hours per day. Banana extract showed significant effect on growth and development of PLBs. Among the treatments, 100 ml L-1 banana extract enhanced new PLBs regeneration from explanted PLBs and growth and development of PLBs. Present research indicated that nutrient requirement for PLBs multiplication and plantlets growth of  Dendrobium orchid is quantitatively different in vitro. Finally, 100 ml L-1 and 25 ml L-1 of banana extract may be recommended as supplement into 1/2MS medium for PLBs multiplication and plantlet regeneration, respectively in vitro.The Agriculturists 2015; 13(1) 101-108

Genetic analysis of in vitro plant tissue culture responses and regeneration capacities

Euphytica ◽  
1994 ◽  
Vol 79 (1-2) ◽  
pp. 45-58 ◽  
Author(s):  
Y. Henry ◽  
P. Vain ◽  
J. De Buyser
Keyword(s):  
Tissue Culture ◽  
Genetic Analysis ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
In Vitro Plant

Effect of exchange of gases for tissue culture vessels to produce the meristem of Solanum tuberosum in vitro

2019 ◽  
Vol 9 (o3) ◽  
Author(s):  
Malek Hubaish Walli ◽  
Haider Mahmooed Jasim ◽  
Faiq Hassan Al
Keyword(s):  
Tissue Culture ◽  
Solanum Tuberosum ◽  
Gas Exchange ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Growth And Development ◽  
Two Factors ◽  
Long Time

The microenvironment in plant tissue culture vessels has a significant effect on the growth and development of plantlets in vitro. The Studies have indicated the gas exchange between outside and inside air can effect on microenvironment of culture vessels, therefore, the experiment was conducted to determine the effect of gas exchange on the production of the fabric tissue to micropropagation of Solanum tuberosum and the possibility of storage the fabric to a long time without drought to the culture. In the experiment, two factors were used. The first factor is the volume of gas exchange of the diameter of the filter (1 cm, 1.5 cm, 2 cm, 2.5 cm) and the second factor used four varieties of Solanum tuberosum (Eastma, Red Scarlet, Arora, Violet ).

scholarly journals In Vitro Plant Tissue Culture: Means for Production of Passiflora Species

2020 ◽  
Vol 4 (4) ◽  
pp. 505-523
Author(s):  
Boboc Oros Paula ◽  
◽  
Timea Hitter Buru ◽  
Corina Cătană ◽  
Maria Cantor ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
In Vitro Plant
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