scholarly journals Effect of Banana Extract on Growth and Development of Protocorm Like Bodies in Dendrobium sp. orchid

2016 ◽  
Vol 13 (1) ◽  
pp. 101-108 ◽  
Author(s):  
M Obaidul Islam ◽  
Md. Serazul Islam ◽  
Md Abu Saleh
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Growth And Development ◽  
Basal Medium ◽  
Plantlet Regeneration ◽  
Nutrient Requirement ◽  
Effect On Growth

An experiment was carried out in Plant Tissue Culture Laboratory, Department of Crop Botany, Bangladesh Agricultural University, Mymensingh to investigate the effect of banana extract on micropropagation of  <i>Dendrobium</i> sp. var. Sonia orchid through PLBs. The experiment was conducted during July 2012 to October 2013. Half-Murashige and Skoog (1/2MS) medium were used as basal medium and the medium was supplemented with banana extract at 12.5, 25, 50, 100, and 200 ml L-1 with a control, where no banana extract was supplemented. The cultures were done in 100 ml conical flasks and maintain at 25°C with 30µ mol m-2 S-1 lighting provided by florescent tubes for 16 hours per day. Banana extract showed significant effect on growth and development of PLBs. Among the treatments, 100 ml L-1 banana extract enhanced new PLBs regeneration from explanted PLBs and growth and development of PLBs. Present research indicated that nutrient requirement for PLBs multiplication and plantlets growth of  Dendrobium orchid is quantitatively different in vitro. Finally, 100 ml L-1 and 25 ml L-1 of banana extract may be recommended as supplement into 1/2MS medium for PLBs multiplication and plantlet regeneration, respectively in vitro.The Agriculturists 2015; 13(1) 101-108

scholarly journals Micropropagation and nursery at garden of Dendrobium caesar

2019 ◽  
Vol 2 (3) ◽  
pp. 14-22
Author(s):  
Quang Minh Pham ◽  
Kien Cong Duong ◽  
Phuong Ngo Diem Quach ◽  
Minh Thi Thanh Hoang
Keyword(s):  
Tissue Culture ◽  
Sodium Hypochlorite ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Growth And Development ◽  
Active Charcoal ◽  
Established Method ◽  
Coconut Husk ◽  
In Vitro Plant

Dendrobium is one of the largest and most important orchid genera because of their ornamental and commercial value. Plant tissue culture is an established method for the effective micropropagation of of valuable plants. In this study, Dendrobium caesar keikis were sterilized with sodium hypochlorite 2.5 % in 15 minutes. Effects of 3 kinds of cytokinin (TDZ, BA and kinetin), adenine and 3 kinds of auxin (IAA, IBA, NAA) with different concentrations on inducing shoots and roots were studied. The highest shoot initation was observed on Knudson C (KC) medium supplemented with BA 2.0 mg/L. Strong roots were induced in KC media supplied with 0.5 g/L active charcoal (AC) and 1.0 mg/L IAA. In order to transfer in vitro plant to the nursery garden, the highest percentage of survival seedling was shown on coconut husk substrate, and peanut husk was the best for the growth and development of Dendrobium caesar. Micropropagation and nursery at garden for Dendrobium caesar were successfully established.

Effect of exchange of gases for tissue culture vessels to produce the meristem of Solanum tuberosum in vitro

2019 ◽  
Vol 9 (o3) ◽  
Author(s):  
Malek Hubaish Walli ◽  
Haider Mahmooed Jasim ◽  
Faiq Hassan Al
Keyword(s):  
Tissue Culture ◽  
Solanum Tuberosum ◽  
Gas Exchange ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Growth And Development ◽  
Two Factors ◽  
Long Time

The microenvironment in plant tissue culture vessels has a significant effect on the growth and development of plantlets in vitro. The Studies have indicated the gas exchange between outside and inside air can effect on microenvironment of culture vessels, therefore, the experiment was conducted to determine the effect of gas exchange on the production of the fabric tissue to micropropagation of Solanum tuberosum and the possibility of storage the fabric to a long time without drought to the culture. In the experiment, two factors were used. The first factor is the volume of gas exchange of the diameter of the filter (1 cm, 1.5 cm, 2 cm, 2.5 cm) and the second factor used four varieties of Solanum tuberosum (Eastma, Red Scarlet, Arora, Violet ).

Media derived from brown seaweeds Cystoseira myriophylloides and Fucus spiralis for in vitro plant tissue culture

2016 ◽  
Vol 128 (2) ◽  
pp. 437-446 ◽  
Author(s):  
Siham Esserti ◽  
Mohamed Faize ◽  
Lalla Aicha Rifai ◽  
Amal Smaili ◽  
Malika Belfaiza ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Brown Seaweeds ◽  
Fucus Spiralis ◽  
In Vitro Plant

A method to rescue the polluted explant in banana (Musa spp) tissue culture

2012 ◽  
Vol 2 (4) ◽  
pp. 157-161
Author(s):  
Chang S ◽  
Li J ◽  
Sun W ◽  
Lin F ◽  
Xu B
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Basal Medium ◽  
Banana Production ◽  
Musa Spp

Plant tissue culture is an important commercial tool in banana production. Sterilization is the first key step in plant tissue culture. When explants were found infected, they were always discarded. But if the materials were precious and rare, once they were cast away, it will be very difficult to get them again. No paper has been found to resolve this problem. In this paper, two precious banana plantlets were polluted during the process of tissue culture. The leaves and the stem were cut from the polluted seedlings. Only the pseudostem was left. The black and brown part of the pseudostem were also cut and discarded. The modified pseudostem was soaked in 6% NaClO solution 10 minutes first, and then, it was kept in 70% ethanol for 1.5 minutes. After that, the pseudostem was cultured on MS basal medium supplemented with sucrose, 7 g L‐1 agar and 3 mg/L 6‐Benzylaminopurine (6‐BA). Most of the explants grow healthily during the following time.

scholarly journals In vitro plant tissue culture: means for production of biological active compounds

Planta ◽  
2018 ◽  
Vol 248 (1) ◽  
pp. 1-18 ◽  
Author(s):  
Claudia A. Espinosa-Leal ◽  
César A. Puente-Garza ◽  
Silverio García-Lara
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Active Compounds ◽  
In Vitro Plant

scholarly journals Endogenous Bacterial Contamination of Plant Tissue Culture Materials: Identification and Control Strategy

2018 ◽  
Vol 28 (1) ◽  
pp. 99-108 ◽  
Author(s):  
Mohammad Ali ◽  
Shefali Boonerjee ◽  
Mohammad Nurul Islam ◽  
Mihir Lal Saha ◽  
M Imdadul Hoque ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Bacterial Contamination ◽  
Antibiotic Sensitivity ◽  
Sensitivity Test ◽  
Bacterial Isolates ◽  
Culture And Sensitivity ◽  
And Control

The endogenous bacterial contamination of plant tissue culture materials and their possible control was studied. Nine bacterial isolates were isolated from the contaminated tissue culture materials viz. potato and tea. On the basis of morphology and biochemical characters of nine isolates, seven were identified as Gram positive belonging to Bacillus alcalophilus, B. circulans, B. infantis, B. lentus, B. schlegelii, B. pumilus and B. subtilis. Remaining two were Gram negative and identified as Enterobacter cloacae sub. sp. dissolvens and Pantoea agglomerans. Molecular analysis was conducted on the basis of 16S rDNA sequence to confirm three isolates. Culture and sensitivity test was carried out to screen out the antibiotic sensitivity where streptomycin (S-10), polymyxin (PB-300) and gentamicin (CN-120) antibiotics were found to be effective against all bacterial isolates. The culture and sensitivity test reflected the feasibility to control or eliminate the contaminant bacteria during in vitro culture of plant which is very much required in the commercial tissue culture production.Plant Tissue Cult. & Biotech. 28(1): 99-108, 2018 (June)

scholarly journals In vitro production of capsaicin through plant tissue culture

2017 ◽  
pp. 24-33
Author(s):  
Swetnisha, Ajitabh Bora, H.K. Gogoi, P.S. Raju
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
In Vitro Production ◽  
Agricultural Produce ◽  
Medicinal Properties ◽  
Method Of Production ◽  
Culture Strategies ◽  
Vitro Production

Capsaicin, a secondary metabolite produced in capsicum, is in high demand in pharmaceutical industry because of its various medicinal properties. Currently, the supply of capsaicin depends upon its extraction from capsicum fruits. This limits the production of capsaicin as it depends upon agricultural produce. The current review has compiled information from various literature published on chemistry and importance of capsaicin along with its method of production. It also reviews the process of in vitro production of capsaicin through plant tissue culture, strategies of increasing capsaicin accumulation and its advantages over extraction from fruits and artificial synthesis.

scholarly journals Sodium Dichloroisocyanurate: An eco-friendly chemical alternative for media autoclaving and explant sterilisation in plant tissue culture

2021 ◽  
Vol 12 (1) ◽  
pp. 107-112
Author(s):  
Simran Chandrahas Shetty ◽  
Narasimhan S
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Active Agent ◽  
Electrical Energy ◽  
Life Forms ◽  
Water Soluble ◽  
Viable Alternative ◽  
Sodium Dichloroisocyanurate

Autoclaving nutrient media is still considered as the optimum mode of sterilisation in plant cell and tissue culture. During the process steam under high pressure is maintained at 120 degrees Celsius, 15 psi for 15-20 minutes in a chamber, optimised to kill all possible microbial life forms. But the disadvantages related to the process of autoclaving are plentiful. They are, decrease in the media pH, salt precipitation, agar depolymerisation, carbohydrate hydrolysis, volatile obliteration and necessity of the infrastructure investment. Requirements of additional resources (time, human resources, electrical energy) have forced the lookout for a more viable alternative, that is, chemical sterilisation. The use of Sodium dichloroisocyanurate (NaDCC) is a useful alternative for media and explant sterilisation. NaDCC is stable, water-soluble, non-toxic and easy to use at room temperature, does not have any environmental hazards and is not phytotoxic. The use of NaDCC as a disinfectant has been documented well concerning water sterilisation, surface sterilisation and also as a broad spectrum disinfecting agent. Disinfecting property of NaDCC is due to the hydrolytic release of chlorine, and this can be utilised for sterilisation of media and explants in plant tissue culture. NaDCC is a useful alternative for autoclaving at a concentration range of 0.05 to 1.0 g/l. However, only a few reports are available for its use as a sterilising agent for media and explants for in vitro cultures of plants. This paper discusses and reviews the possibility of establishing NaDCC as an active agent for explant sterilisation and as a viable alternative to medium sterilisation through autoclaving.

scholarly journals IN VITRO SHOOT MULTIPLICATION OF STEVIA REBAUDIANA (BERT) THROUGH PLANT TISSUE CULTURE.

2016 ◽  
Vol 4 (11) ◽  
pp. 2300-2307
Author(s):  
Vibha Bhingradiya ◽  
◽  
Archana Mankad ◽  
Ruby Patel ◽  
Shivangi Mathur ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Stevia Rebaudiana ◽  
Shoot Multiplication
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