scholarly journals FUNCTIONAL STATE OF THE MARINE MICROALGAE CULTURES AS AN INDICATOR OF THE WATER POLLUTION LEVEL OF THE SEVASTOPOL BAY

2021 ◽  
pp. 83-90
Author(s):  
L.V. Stelmakh ◽  
◽  
I.M. Mansurova ◽  
Keyword(s):  
Functional State ◽  
Black Sea ◽  
Phaeodactylum Tricornutum ◽  
Inhibitory Effect ◽  
The Black Sea ◽  
Pollution Level ◽  
Sevastopol Bay ◽  
Algae Growth ◽  
Taxonomic Affiliation

The possibility of using cultures of Black Sea planktonic microalgae of different taxonomic affiliation to assess the quality of the aquatic environment based on the assessment of their functional state is shown. The research was carried out on the diatom Phaeodactylum tricornutum, three dinoflagellates species Prorocentrum cordatum, Prorocentrum pusillum and Gyrodinium fissum, as well as the coccolithopho-ride Emiliania huxleyi. It was found that the waters of the Sevastopol Bay in the period from May to August 2021 in 50% of cases had a weak inhibitory effect on the growth of the tested species. In other cases, algae growth was either stimulated or the effect of pollution on cultures was not revealed. The need for using several types of algae in carrying out complex work on water biotesting of the coastal are-as of the Black Sea is highlighted.

Response of marine microalgae Phaeodactylum tricornutum, Prorocentrum cordatum and Gyrodinium fissum to complex pollution of Sevastopol bays (Black Sea)

2021 ◽  
Vol 48 ◽  
pp. 109-116
Author(s):  
Liudmila Stelmakh ◽  
Nela Kovrigina ◽  
Tatiana Gorbunova
Keyword(s):  
Black Sea ◽  
Coastal Waters ◽  
Phaeodactylum Tricornutum ◽  
Laboratory Experiments ◽  
Toxicity Assessment ◽  
Marine Microalgae ◽  
The Black Sea ◽  
Organic Substances ◽  
Taxonomic Affiliation ◽  
Concentration Levels

For the degrees of toxicity assessment within the Black Sea surface coastal waters near Sevastopol, laboratory experiments were performed on three marine microalgae species culture’s growth in water sampled on the three stations of the region from May to December 2020. The study has indicated, that in the majority of cases, insignificant inhibiting influences of the waters to diatom Phaeodactylum tricornutum Bohlin and insignificant stimulated effect to dinoflagellates Prorocentrum сordatum (Ostenfeld) J.D.Dodge and Gyrodinium fissum (Levander) Kofoid & Swezy were observed. Rarely this effect was characterized as chronic or subacute. The concentration levels of both stable and unstable forms of organic substances in the studied waters were estimated. The results of the testing of the water to determine its contamination using microalgae and analysis of the organic substances concentration levels in them, allowed the conclusion that the studied area should be classified as episodically polluted. Justification for the necessity to use several microalgae species of different taxonomic affiliation when performing marine water biotesting is provided in this study.

Oithona davisae: naturalization in the Black sea, inter-annual and seasonal dynamics, effect on the structure of the planktonic copepod community

2019 ◽  
Vol 59 (6) ◽  
pp. 1008-1015
Author(s):  
A. D. Gubanova ◽  
O. A. Garbazey ◽  
D. A. Altukhov ◽  
V. S. Mukhanov ◽  
E. V. Popova
Keyword(s):  
Black Sea ◽  
Coastal Waters ◽  
Native Species ◽  
Significant Decline ◽  
Acartia Tonsa ◽  
The Black Sea ◽  
Copepod Community ◽  
Oithona Davisae ◽  
Sevastopol Bay

Long-term (20032014) routine observations of zooplankton in Sevastopol Bay (the Black Sea) have allowed the naturalization of the invasive copepod Oithona davisae to be studied in the Black Sea coastal waters. Inter-annual and seasonal variability of the species and their impact on the native copepod community have been analyzed. The invasion of O. davisae and their undoubted dominance in terms of abundance were shown to alter the community structure but, at the same time, the abundances of the native species did not decrease, excepting the Black Sea earlier invader Acartia tonsa. A significant decline in A. tonsa numbers over the stages of O. davisae establishment and naturalization provided evidence of competition between the species. O. davisae have been demonstrated to gain competitive advantage over A. tonsa, that ensured their fast dispersal in the Black Sea, acclimatization in the new habitat and the successful competition over native species.

Earth Observation products  for Science and Innovation in the Black Sea

2021 ◽  
Author(s):  
Marilaure Grégoire ◽  
Keyword(s):  
Black Sea ◽  
Earth Observation ◽  
Sea Surface Salinity ◽  
The Black Sea ◽  
Full Potential ◽  
Ocean Colour ◽  
Surface Salinity ◽  
Mesoscale Dynamics

<p>The Black Sea is a small enclosed basin where coastal regions have a large influence and mesoscale signals dominate the dynamics (the Rossby radius of deformation is about 20km). Large riverine inputs, mainly on the northwestern shelf, induce well-marked horizontal gradients in the distribution of the Black Sea salinity and optical characteristics: coastal and shelf waters have very low salinity and contain large amounts of optically active materials (e.g. coloured dissolved organic matter) and its oligotrophic deep sea has a salinity around 18.2. The presence of these contrasting water characteristics in a relatively small enclosed environment, combined with land contamination and the specificities of its atmospheric composition(e.g. high cloud coverage, aerosols) make the Black Sea a challenging area for the development of high quality satellite products. </p><p> </p><p>We present first results from a 2-year on-going ESA-funded project, EO4SIBS (Earth Observation for Science and Innovation in the Black Sea) dedicated to the development, and subsequent scientific analysis, of new algorithms and products. In particular, ocean colour products (chlorophyll-a and total suspended matter concentrations, turbidity) were produced from Sentinel 3 (S3) OLCI data combining different algorithms selected based on an automatic water mass classification procedure (case-1 versus case-2 waters). In specific areas, S3-OLCI and Sentinel 2-MSI data were merged to address local features. A revised gridded altimetry product based on 5-Hz along track data (combining Cryosat and S3 SAR) was produced and validated in the coastal zone with tide gauge data. Sea Surface Salinity was derived from the L-Band measured by SMOS and compared with in-situ surface salinity data from field sampling and Argo. </p><p> </p><p>All these products are now being integrated to further understand the Black Sea physical and biogeochemical functioning (e.g., plume and productivity patterns, mesoscale dynamics, deoxygenation). For instance, the Black Sea mesoscale dynamics are inferred from the 5-Hz altimetry product using an eddy detection and tracking algorithm. The quality of the eddy mapping is assessed by comparison with visible and infrared satellite products while the derived velocities are compared with drifters. Also, the benefit of assimilating ocean colour data in the Black Sea operational model (also known as CMEMS BS-MFC BIO) for the prediction of the Black Sea ecosystem will  be illustrated.</p><p> </p><p>Gridded products are archived as CF-compliant NetCDF files and disseminated through ncWMS protocol. In-situ data are modeled as vector points in a PostGIS database. A web portal is being implemented in order to propose an efficient spatiotemporal exploration of both data sources in a user-friendly interface, including interactive map layers and export possibilities.</p><p> </p><p>We conclude with a set of recommendations for observational requirements needed  to increase the quality of satellite products in the Black Sea and to be able to use the full potential of current and new information provided by  satellites. </p><p> </p>

Organic Matter of Bottom Sediments of the Crimean and Caucasian Coasts (Azov and Black Seas)

2021 ◽  
Author(s):  
E. A. Tikhonova ◽  
Keyword(s):  
Organic Matter ◽  
Black Sea ◽  
Bottom Sediments ◽  
Petroleum Hydrocarbons ◽  
Water Area ◽  
The Black Sea ◽  
Pollution Level ◽  
Sea Of Azov ◽  
The Caucasus ◽  
The Sea Of Azov

As part of the 113th cruise of the R/V “Professor Vodyanitsky”, research was conducted on organic pollution of bottom sediments in the coastal areas of Crimea and the Caucasus, as well as the water area in front of the Kerch Strait. Concentration of chloroformextractable substances was determined by the weight method and that of petroleum hydrocarbons was determined using infrared spectrometry. Both in 2020 and 2016 (the 83d cruise of the R/V “Professor Vodyanitsky”), properties of the bottom sediments of the Crimean and Caucasian coasts were typical of the marine soils of this region. This indicates that the studied water areas are generally in good condition. In accordance with the regional classification of bottom sediment pollution, the maximum concentrations of chloroform-extractable substances obtained for both the Black Sea and the Sea of Azov coast indicate pollution level III (23% of analysed samples). These values were found in bottom sediments in the Sevastopol water area (225 mg·100 g-1), in the coastal area of Cape Tarkhankut (120 mg·100 g-1) and Karadag (120 mg·100 g-1), the southern part of the Sea of Azov (125 mg·100 g-1) and Tuapse (110 mg·100 g-1). The content of chloroform-extractable substances in bottom sediments off the Black Sea coast of the Caucasus and the Sea of Azov coast is slightly lower than that off the Crimean coast. Pollution level II is assigned to bottom sediments in 46 % of the samples, with an average concentration of 72 mg·100 g-1 of air-dry solids. The rest (31 %) of the studied area was classified as conditionally clean (pollution level I, i. e. less than 50 mg·100 g-1). There has been a slight increase in the concentration of petroleum hydrocarbons in the bottom sediments of both the Black Sea and the Sea of Azov and their share in the total amount of chloroformextractable substances. In general, the level of pollution of bottom sediments by organic matter remained unchanged if compared with previous years, in particular with the data from 2016

IMPROVEMENT OF THE BIOLOGICAL EFFECTIVENESS OF FAT FROM THE LIVER OF THE BLACK SEA SHARK-KATRAN

2021 ◽  
Author(s):  
Е.Е. ИВАНОВА ◽  
Е.А. ДУБИНЕЦ ◽  
А.Л. БОЧАРОВА-ЛЕСКИНА
Keyword(s):  
Fatty Acids ◽  
Iodine Number ◽  
Black Sea ◽  
Liquid Fraction ◽  
Saturated Fatty Acids ◽  
Biologically Active ◽  
The Black Sea ◽  
Omega 3 ◽  
Biological Effectiveness

Для стабилизации показателя биологической эффективности исследована возможность повышения количества полиненасыщенных жирных кислот (ПНЖК) за счет снижения количества насыщенных жирных кислот (НЖК) в жирнокислотном составе жира из печени черноморской акулы-катрана в летний период вылова (с мая по октябрь). С целью повышения содержания омега-3 ПНЖК в продукте применен способ винтеризации. Кристаллизацию жира из печени акулы-катрана проводили в специальном охладителе при температуре от 0 до –5°С. Во время охлаждения жир медленно перемешивали (скорость вращения мешалки 20–25 об/мин) для равномерного охлаждения по всему объему. Полная кристаллизация НЖК при этих условиях проходила за 5–6 ч. Охлажденный жир немедленно направляли на осадительную центрифугу. В качестве параметров оптимизации процесса были выбраны: качество жира – прозрачность при температуре 15°С и степень ненасыщенности – йодное число жира. Установлено, что факторами, существенно влияющими на йодное число и органолептические показатели жира, являются: температура охлажденного жира, продолжительность центрифугирования, число оборотов барабана центрифуги. Для моделирования значений йодного числа и органолептического показателя в области изменения указанных факторов применена схема полного факторного двухуровневого эксперимента, в результате которого определены натуральные значения факторов, отвечающие наилучшему значению показателя качества жира: температура охлаждения жира –4°С, продолжительность центрифугирования 30 мин, частота вращения барабана центрифуги 6000 об/мин. Общая ненасыщенность жидкой фракции возросла с 122,2–138,0 до 131,0–145,0% йодного числа жира. Полученный очищенный жир был прозрачен, имел светло-желтый цвет, низкие значения кислотного и перекисного чисел. Содержание ПНЖК в полученном продукте увеличилось на 10–30%. Содержание неомыляемых веществ в нем, в том числе биологически активных веществ алкилглицеролов, при этом не снизилось. Биологическая эффективность жира увеличилась с 0,6 до 0,9. To stabilize the increased biological effectiveness the possibility of increasing the amount of polyunsaturated fatty acids (PUFA) by reducing the amount of saturated fatty acids (SFA) in the fatty acid composition of fat from the liver of the black sea shark-katran in the summer fishing period (May – October) is investigated. With the aim of increasing the content of omega-3 PUFA in the product method of winterization was applied. Crystallization of fat from the liver of sharks-katran dogfish was conducted in a special chiller with temperature from 0 to –5°C. During cooling, the fat is slowly stirred (speed of rotation of the mixer 20–25 rpm) for uniform cooling throughout. Full crystallization of SFA under these conditions was held for 5–6 h. The cooled fat was immediately sent to precipitating in the centrifuge. Quality of fat – transparency at a temperature of 15°C and unsaturation – iodine number of fat was selected as the optimization parameters of the process. It is established that: the temperature of the refrigerated fat, the duration of centrifugation, the number of drum rotations of the centrifuge are factors that significantly affect the iodine number and the organoleptic indicators of fat. Scheme two-level full factorial experiment was applied for modeling the values of iodine number and organoleptic indicator in changing these factors. Natural values of factors corresponding to the best value of indicator of the quality of the fat: temperature of cooling fat –4°C, duration of centrifugation 30 min, and the speed of rotation of the drum centrifuge 6000 rpm were determined in the experiment. The result of this treatment, the total unsaturation of the liquid fraction increased from 122,2–138,0 up to 131,0–145,0% of iodine number. The purified obtained fat was transparent, had a light yellow color, low acid value and peroxide values. In the obtained product the content of PUFA increased by 10–30%. The content of unsaponifiables in it, including biologically active substances alkylglycerols, was not decreased. The biological effectiveness of fat increased from 0,6 to 0,9.

Long-term Changes in Sevastopol Bay (the Black Sea) with Particular Reference to the Ichthyoplankton and Zooplankton

2001 ◽  
Vol 52 (1) ◽  
pp. 1-13 ◽  
Author(s):  
A.D. Gordina ◽  
E.V. Pavlova ◽  
E.I. Ovsyany ◽  
J.G. Wilson ◽  
R.B. Kemp ◽  
...  
Keyword(s):  
Black Sea ◽  
The Black Sea ◽  
Sevastopol Bay ◽  
Long Term Changes
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