scholarly journals In vitro regeneration of Anthurium andreanum cv. Nitta

1970 ◽  
Vol 35 (2) ◽  
pp. 217-226 ◽  
Author(s):  
SA Islam ◽  
MMR Dewan ◽  
MHR Mukul ◽  
MA Hossain ◽  
F Khatun
Keyword(s):  
Root Elongation ◽  
In Vitro Regeneration ◽  
Shoot Formation ◽  
Environmental Conservation ◽  
The Other ◽  
In Vitro Organogenesis ◽  
Shoot Initiation ◽  
Anthurium Andreanum ◽  
Hormone Control

The present study was undertaken to investigate the effect of different combinations of NAA, IBA, and BAP on in vitro organogenesis of Anthurium andreanum cv. "Nitta" at the DEBTEC (Development of Biotechnology & Environmental Conservation Centre) Laboratory, Dhaka. Organogenesis from leaf mid rib to shoot initiation required 9.00 days and the best survivality was 80.00% percent at 30 DAI (Day after initiation) with the combination of 1 mg/L NAA and 1 mg/L BAP in MS media. Among the 20 hormone supplements, MS media containing 1 mg/L NAA and 1 mg/L BAP showed the highest shoot formation (68.30%), number of shoots/explant (3.37) and the longest shoot (4.65 cm) at 60 DAI. MS media without any hormone (control) showed the poorest performance in regeneration of shoots. On the other hand, MS media containing 1 mg/L IBA + 1 mg/L BAP showed the best performance in rooting of shoots (83.85%), highest number of roots (4.29/plantlet), root elongation (5.50 cm) were recorded at 60 DAI. Keywords: In vitro regeneration; Anthurium andreanum. DOI: 10.3329/bjar.v35i2.5884Bangladesh J. Agril. Res. 35(2) : 217-226, June 2010

scholarly journals IN VITRO REGENERATION AND MASS PROPAGATION OF AZIMA TETRACANTHA [LAM.] FROM THE LEAF EXPLANTS THROUGH CALLUS CULTURE

2017 ◽  
Vol 4 (2) ◽  
pp. 52-56
Author(s):  
Mallika Devi T
Keyword(s):  
Callus Induction ◽  
In Vitro Regeneration ◽  
Leaf Explants ◽  
Shoot Formation ◽  
Ms Medium ◽  
Apical Leaf ◽  
Half Strength ◽  
Regenerated Plantlets ◽  
Azima Tetracantha

In the present study the protocol for callus induction and regeneration in Azima tetracantha has been developed in culture medium. The young apical leaf explants were used for callus induction on MS medium containing BAP and NAA at 1.0 and 0.4mgl-1 respectively showed maximum callus induction (73%). The amount of callus responded for shoot formation (74%) was obtained in the MS medium containing BAP (1.5 mgl-1) and NAA (0.3mgl-1).The elongated shoots were rooted on half strength medium supplemented with IBA (1.5 mgl-1) and Kn (0.4 mgl-1) for shoots rooted. Regenerated plantlets were successfully acclimatized and hardened off inside the culture and then transferred to green house with better survival rate.

scholarly journals In vitro Regeneration of the Medicinal Plant, Plumbago zeylanica L. with Reference to a Unique Population in Maruthamalai, the Western Ghats, India

1970 ◽  
Vol 18 (2) ◽  
pp. 173-179 ◽  
Author(s):  
T. Mallikadevi ◽  
P. Senthilkumar ◽  
S. Paulsamy
Keyword(s):  
Medicinal Plant ◽  
In Vitro Regeneration ◽  
Basal Medium ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Garden Soil ◽  
Plumbago Zeylanica ◽  
Adventitious Shoot Formation ◽  
The Western Ghats

The in vitro regeneration of Plubago zeylanica exhibited that the callus was initiated in the basal medium containing BAP, NAA, 2, 4-D, and IBA.  The high amount (90%) of organic calli was induced in the basal medium supplemented with 2, 4-D, alone at 2.0 mg/l. In the subculture the adventitious shoot formation was prominently higher (83%) in the basal medium containing BAP, and NAA at 3.5 and 0.3 mg/l, respectively. IAA (1.0 mg/l)effectively produced higher percen-tage (90) of roots and root growth. After sequential hardening, survivability rate was observed to be significantly higher (80%) in the hardening medium containing garden soil, sand and vermicompost in the ratio of 1 : 1 : 1 by volume under greenhouse condition.  Key words: Plumbago zeylanica, In vitro regeneration, Medicinal plant D.O.I. 10.3329/ptcb.v18i2.3648 Plant Tissue Cult. & Biotech. 18(2): 173-179, 2008 (December)

scholarly journals Long-term in Vitro Regeneration of Hamelia patens

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 873G-874
Author(s):  
D. Sankhla ◽  
T.D. Davis ◽  
N. Sankhla ◽  
A. Upadhyaya
Keyword(s):  
Culture Medium ◽  
In Vitro Regeneration ◽  
Shoot Formation ◽  
Shoot Tips ◽  
Shoot Cultures ◽  
Ms Medium ◽  
Prolific Shoot ◽  
Ornamental Shrub

This report describes an efficient in vitro regeneration protocol for H. patens (firebush), a heat-tolerant ornamental shrub native to tropical and subtropical America. Shoot cultures were initially established using shoot tips placed on MS-revised medium containing 2.3 μM 2,4-D, 2.3 μM kinetin, and 0.25% polyvinylpyrrolidone. Other types of explants (nodal and internodal segments, leaf pieces, floral buds) did not regenerate shoots when placed on this medium. Two-month-old plantlets derived from the shoot tips were subcultured on MS medium supplemented with 0.5 μM thidiazuron (TDZ), and within 3 to 4 weeks, some callus was produced at the root–shoot junction. When this callus, with a small portion of the root and shoots, was placed on MS medium with 0.05 μM TDZ and 0.01 μM ABA, prolific shoot formation occurred within 3 to 4 weeks followed by root formation. By regular subculturing every 5 to 6 weeks, hundreds of plantlets have been obtained over the past 3 years with no apparent decline in regeneration potential. Addition of activated charcoal (0.5%) to the culture medium has greatly improved growth of the plantlets.

scholarly journals In vitro regeneration from cotyledons of watermelon

2000 ◽  
Vol 6 (4) ◽  
Author(s):  
V. Zarka ◽  
L. Velich ◽  
Gy. Bisztray
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
In Vitro Regeneration ◽  
Shoot Formation ◽  
Root Induction ◽  
Free Medium ◽  
Histochemical Assay ◽  
Gus Histochemical Assay ◽  
Scanning Electron

Cotyledonary segments of five different genotypes of watermelon were used to induce organogenesis. Five different hormone combinations were applied to enhance the induction of shoot formation on the surface of the segments. The phases of organogenesis were followed with light and scanning electron microscope. Shoots were obtained after four weeks, then the shoots were transferred to hormone free medium for root induction. This method of regeneration can be applied in transformation experiments. GUS histochemical assay was made to check the expected success of using Agrobacterium for the transformation.

scholarly journals In vitro regeneration in cole crops

1970 ◽  
Vol 8 (2) ◽  
pp. 227-232 ◽  
Author(s):  
Sonia B Shahid ◽  
SZ Khan ◽  
L Hassan
Keyword(s):  
In Vitro Regeneration ◽  
Plantlet Regeneration ◽  
Ms Medium ◽  
Root Initiation ◽  
Cole Crops ◽  
Shoot Initiation ◽  
Callus Initiation ◽  
Callus Proliferation ◽  
Number Of Shoots

The experiment was conducted to optimize a suitable protocol for in vitro regeneration in cole crops. Callus initiation was excellent in the variety Early Tropical. Highest percentage of callus proliferation was observed in Early Tropical (75.0%) followed by Tangail Special Pauslali (55.0%) and the lowest in Tara (40.0% ). Maximum callus proliferation (68.5%) was observed in MS + 3.0 mgL-1 BAP + 0.1 mgL-1 2,4-D + 2.0 mgL-1 AgNO3. Callus proliferation was lowest (40.0%) in MS + 2.5 mgL-1 BAP + 0.1 mgL-1 2,4-D + 2.0 mgL-1 AgNO3. MS medium supplemented with 3.0 mgL-1 BAP + 1.0 mgL-1 2,4-D + 2.0 mgL-1 AgNO3 was the best for shoot initiation & plantlet regeneration. The highest number of shoots per vial was 7.20 and the lowest number of shoots per vial was 4.40. Among the concentration MS + 3.0 mgL-1 BAP + 0.1 mgL-1 2,4-D + 2.0 mgL-1 AgNO3 showed the highest performance of shoots per vial. The variety Tangail Special Pauslali was the best for root initiation. Keywords: Brassica; Cole crops; Callus; In vitro; Regeneration DOI: 10.3329/jbau.v8i2.7930 J. Bangladesh Agril. Univ. 8(2): 227-232, 2010

In Vitro Regeneration of Valencia-type Peanut (Arachis hypogaea L.) from Cultured Petiolules, Epicotyl Sections and Other Seedling Explants1

1992 ◽  
Vol 19 (2) ◽  
pp. 82-87 ◽  
Author(s):  
Ming Cheng ◽  
David C. H. Hsi ◽  
Gregory C. Phillips
Keyword(s):  
In Vitro Regeneration ◽  
Multiple Shoots ◽  
Shoot Formation ◽  
Direct Organogenesis ◽  
Naphthaleneacetic Acid ◽  
Regeneration System ◽  
Arachis Hypogaea L ◽  
Wide Range ◽  
Cultivated Peanut

Abstract This study evaluated plant development via direct organogenesis from in vitro-cultured young seedling tissues of cultivated peanut, especially the valencia-type peanut. Complete plants were regenerated from in vitro-cultured petiolule-with-blade-attached explants, leaflet segments, and epicotyl andpetiole sections. Multiple shoots arose on Murashige and Skoog medium (MS) supplemented with 6-benzylaminopurine (BA) (5–25 mg/L) plus 1-naphthaleneacetic acid (NAA) (0.5–3 mg/L). After 30 d culture on 25 mg/L BA + 1 mg/L NAA, 1.6 buds or shoots/explant were regenerated from the petiolule-with-blade-attached explants. Comparable numbers of shoots were obtained from epicotyl sections of the first node region of the seedling after 60 d culture using 10 mg/L BA + 1 mg/L NAA. Leaflet segments and petiole sections were less responsive for shoot formation. Excised shoots developed roots in vitro upon transfer for 15 d to MS medium supplemented with NAA at 1 mg/L. Plantlets were transferred to soil and grown in a greenhouse to maturity. A wide range of cultivated peanut genotypes was evaluated for organogenic responsiveness, using the petiolule-with-blade-attached explant source. Only valencia-type cultivars, or a hybrid derivative with a Valencia background, were responsive with this regeneration system.

scholarly journals Establishment of in vitro regeneration protocol for Adhatoda vasica Nees

2016 ◽  
Vol 51 (1) ◽  
pp. 75-80 ◽  
Author(s):  
S Khan ◽  
S Akter ◽  
A Habib ◽  
TA Banu ◽  
M Islam ◽  
...  
Keyword(s):  
In Vitro Regeneration ◽  
Multiple Shoots ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Nodal Segments ◽  
Garden Soil ◽  
Root Induction ◽  
Ms Medium ◽  
Adhatoda Vasica

An in vitro regeneration protocol of Adhatoda vasica has been developed using excised nodal segments and juvenile leaves for multiple shoots regeneration directly or through callus induction. Explants were cultured on MS medium with different concentrations of IAA, NAA, BAP, GA3 and Kn singly or in combinations. MS medium supplemented with BAP (10.0 mg/l) was found best for multiple shoot formation, in which 93.33% explants produced multiple shoots. After two months, maximum number of multiple shoots were 10.6 ± 1.82, highest length of plantlets was 5.2 ± 2.20 cm. 100% calli formation were observed on MS medium supplemented with IAA (0.05 mg/l) + NAA (0.05 mg/l) + BAP (1.0 mg/l). Callus initiation started after 14 days and gave light green colored callus. Best callus mediated shoot regeneration was found on MS+10.0 mg/l BAP medium. Root induction of in vitro raised shoots was best on ½ MS + IBA (1.0 mg/l). Well rooted plantlets were transferred to plastic pots containing garden soil and compost in a ratio of 2:1 for hardening. The ultimate survival rate under natural condition was about 80%.Bangladesh J. Sci. Ind. Res. 51(1), 75-80, 2016

scholarly journals In Vitro Regeneration through Direct Organogenesis from Protocorms of Oncidium tigrinum Llave & Lex. (Orchidaceae), an Endemic and Threatened Mexican Species

HortScience ◽  
2011 ◽  
Vol 46 (8) ◽  
pp. 1132-1135 ◽  
Author(s):  
Martín Mata-Rosas ◽  
Rosario Julieta Baltazar-García ◽  
Victor Manuel Chávez-Avila
Keyword(s):  
Culture Media ◽  
In Vitro Regeneration ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Direct Organogenesis ◽  
Naphthaleneacetic Acid ◽  
Average Height ◽  
Ms Medium ◽  
Adventitious Shoot Formation

A protocol for in vitro propagation from protocorms of Oncidium tigrinum Llave & Lex., a threatened species distributed in Mexico and highly appreciated as an ornamental, was developed. Two different explants, entire protocorms and longitudinal halves of protocorms, were used. In addition, the effect of two different culture media, Murashige and Skoog (MS) and modified Knudson (KCm), supplemented with N6-benzyladenine (BA) (0, 0.5, 1, 2, 3, and 5 mg·L−1) and/or α-naphthaleneacetic acid (NAA) at 0, 0.1, and 0.5 mg·L−1 was investigated. Adventitious shoot formation by direct organogenesis was obtained in all treatments; in some cases, the formation of protocorm-like bodies was induced. Shoot formation was greater for entire protocorms; the best treatment was MS medium containing at BA 1 to 2 mg·L−1 in combination with at NAA 0.1 mg·L−1. The average height of shoots was three times greater in MS medium than in KCm medium. Subculturing individual shoots in MS medium without plant growth regulators, but with 1 g·L−1 activated charcoal, allowed further development and rooting. An ex vitro survival rate of almost 100% was achieved. This study represents a comprehensive application for propagation, conservation, and sustainable use of this valuable natural resource.

scholarly journals AgNO3 Promotes Callus Production and Regeneration of Immature Buffalograss Inflorescence Culture

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 631c-631
Author(s):  
Shuizhang Fei ◽  
Paul E. Read ◽  
Terrance P. Riordan
Keyword(s):  
Callus Formation ◽  
In Vitro Regeneration ◽  
Shoot Formation ◽  
Positive Effects ◽  
Friable Embryogenic Callus ◽  
Friable Callus ◽  
Promotive Effect ◽  
Callus Production ◽  
Regeneration Efficiency

The use of buffalograss [Buchloe dactyloides (Nutt.) Engelm] in home lawns and golf courses has been increasing because of its drought resistance and low growth habit. In vitro regeneration of buffalograss at a high frequency may provide an effective tool to introduce new variation for breeding use. The positive effects of AgNO3 on friable embryogenic callus production and regeneration efficiency is well documented in maize. In order to determine if AgNO3 has the same effect on buffalograss, two vegetatively propagated cultivars, a female `609' and a male `45-3' were tested at three different concentrations of AgNO3 at 5, 10, and 15 mg·L–1 using immature inflorescences as explants. Murashige and Skoog medium supplemented with 2 mg 2,4-D/L was employed as the control medium. Medium containing AgNO3 significantly promoted the production of friable callus for `45-3' with the highest percentage achieved at 10 mg AgNO3/L. AgNO3 medium led to production of significantly larger calli than found for the control. However, no difference was detected among 5, 10, and 15 mg AgNO3/L with regard to the callus formation ability and the size of callus initiated on these three treatments. Calli were then transferred to MS medium supplemented with BA at 0.1, 0.5 or 1.0 mg·L–1 to induce shoot formation. BA at 0.5 mg·L–1 gave the best differentiation response. Calli formed in the absence of AgNO3 produced more shoots per callus, but more calli were produced in the presence of AgNO3, and the overall regeneration efficiency was much higher with AgNO3 at 10 mg·L–1. In contrast, AgNO3 showed no promotive effect on callus production and regeneration for `609'.

scholarly journals Improved Adventitious Shoot Production in White Ash

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 874C-874
Author(s):  
Sharon A. Bates ◽  
John E. Preece ◽  
John H. Yopp
Keyword(s):  
Adventitious Shoot ◽  
Shoot Elongation ◽  
Shoot Formation ◽  
Separate Experiment ◽  
Ms Medium ◽  
Adventitious Shoot Formation ◽  
White Ash ◽  
Shoot Initiation ◽  
Shoot Production

To increase adventitious shoot formation, we investigated the effects of the number of weeks on medium with high levels of plant growth regulators and seedcoat removal. Dissected white ash seeds were placed on a solidified MS medium containing 10 μM TDZ and 1 μM 2,4-D (shoot initiation medium). After 2, 3, or 4 weeks in vitro, explants were transferred to shoot elongation medium (3 μM TDZ, 1 μM BA, and 1 μM IBA). After 12 weeks, the greatest number (1.8) and longest shoots (18.7 mm) were in cultures incubated on the shoot formation medium for 3 weeks. In a separate experiment, dissected seeds were placed on shoot formation medium. Seedcoats were removed after 10 days in vitro. Explants were transferred to shoot elongation medium after 4 weeks in vitro. There were more shoots (2.5) on 12-week-old explants without seedcoats than on explants with seedcoats (0.9). This result may be related to inhibitors in the testa.

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