scholarly journals In vitro regeneration from cotyledons of watermelon

2000 ◽  
Vol 6 (4) ◽  
Author(s):  
V. Zarka ◽  
L. Velich ◽  
Gy. Bisztray
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
In Vitro Regeneration ◽  
Shoot Formation ◽  
Root Induction ◽  
Free Medium ◽  
Histochemical Assay ◽  
Gus Histochemical Assay ◽  
Scanning Electron

Cotyledonary segments of five different genotypes of watermelon were used to induce organogenesis. Five different hormone combinations were applied to enhance the induction of shoot formation on the surface of the segments. The phases of organogenesis were followed with light and scanning electron microscope. Shoots were obtained after four weeks, then the shoots were transferred to hormone free medium for root induction. This method of regeneration can be applied in transformation experiments. GUS histochemical assay was made to check the expected success of using Agrobacterium for the transformation.

scholarly journals Shoot induction and plant regeneration from cotyledon segments of the muskmelon variety "hógolyó"

1999 ◽  
Vol 5 (1-2) ◽  
Author(s):  
Cs. Bársony ◽  
Gy. Bisztray ◽  
E. Bába ◽  
E. Velich
Keyword(s):  
Electron Microscope ◽  
Plant Regeneration ◽  
Scanning Electron Microscope ◽  
High Frequency ◽  
Shoot Formation ◽  
Root Induction ◽  
Shoot Induction ◽  
Free Media ◽  
Scanning Electron

Cotyledonary segments of the casaba type muskmelon variety "Hógolyó" were used to induce organogenesis. Fifty different hormone combinations were applied to enhance the induction of shoot formation on the edge of the segments. The phases of organogenesis were followed with light- and scanning electron microscope. Shoot induction was achieved with high frequency. The shoots were transferred to hormone free media for root induction. The rooted plantlets were planted out to soil. NAA was feasible and the method can be applied in transformation experiments.  

Application of Transmission Electron Microscope and Scanning Electron Microscope in experimental tumor studies

1990 ◽  
Vol 48 (3) ◽  
pp. 306-307
Author(s):  
Gao Fengming
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Malignant Transformation ◽  
Transmission Electron Microscope ◽  
Experimental Tumor ◽  
Transmission Electron ◽  
Embryo Cells ◽  
Tumor Studies ◽  
Scanning Electron

Transmission electron microscope(TEM) and scanning electron microscope(SEM) were widely used in experimental tumor studies. They are useful for evaluation of cellular transformation in vitro, classification of histological types of tumors and treating effect of tumors. We have obtained some results as follows:1. Studies on the malignant transformation of mammalian cells in vitro. Syrian golden hamster embryo cells(SGHEC) were transformed in vitro by ThO2 and/or ore dust. In a few days after dust added into medium, some dust crystals were phagocytized. Two weeks later, malignant transformation took place. These cells were of different size, nuclear pleomorphism, numerous ribosomes, increasing of microvilli on cell surface with various length and thickness, and blebs and ruffles(Figs. 1,2). Myelomonocytic leukemic transformation of mouse embryo cells(MEC) was induced in vitro by 3H-TdR. Transformed cells were become round from fusiform. The number of mitochondria and endoplasmic reticulum was reduced, ribosomes and nucleoli increased, shape of nuclei irregular, microvilli increased, and blebs and ruffles appeared(Fig. 3).

Comparative evaluation of surface roughness and texture of polished nanofill and nanohybrid composites using profilometer and scanning electron microscope: in - vitro study

2017 ◽  
Vol 9 (2) ◽  
pp. 4-10
Author(s):  
Krishna Prasada L ◽  
Jyothsna S Jathanna ◽  
Naveen Kumar ◽  
Ramya M.K ◽  
Elizabeth Issac
Keyword(s):  
Surface Roughness ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Resin Composite ◽  
In Vitro Study ◽  
Average Value ◽  
Three Samples ◽  
Group B ◽  
Scanning Electron

BACKGROUND: To determine the effectiveness of three polishing systems on three different composite materials by evaluating surface roughness using a Profilometer and Scanning electron microscope. METHODOLOGY: A total of Sixty-three resin composite disks were prepared in rectangular acrylic mould of 8×2mm dimension, with 4mm thickness. Specimens were made of light activated resin composite Filtek Z-250-XT, Tetric-N-Ceram bulkfill, Ceram X Duo. The sixty-three samples were divided into three groups of twenty-one samples each i.e. Group A (Filtek Z-250-XT), Group B (TetricN-Ceram bulkfill) and Group C (Ceram-X-Duo).Out of 21 samples of each of the material, 7 specimens were polished with multi enhance polishing agents, 7 specimens were polished with super snap polishing agents and 7 specimens were polished with sof-lexdiscs.Surface roughness of each sample after polishingwasevaluatedusingProfilometer and Scanning electron microscope. RESULTS: One-way anova and kruskalwallis test was used for statistical analysis. Ceram-X-Duo gave least roughness average value with Super snap polishing system when compared to multi enhance and sof-lex polishing system. CONCLUSION: Super snap polishing system is a better polishing system than multi enhance and soflex and Ceram-X-Duo composite material offers better polishability compared to Filtek Z-250-XT and Tetric-N-Ceram bulk fill.

scholarly journals In vitro Regeneration and Agrobacterium-mediated Transformation of Potato (Solanum tuberosum L.) Cultivars Grown in Mexico

2013 ◽  
Vol 22 (2) ◽  
pp. 93-105 ◽  
Author(s):  
Rose Onamu ◽  
Juan P Legaria ◽  
Jaime C Sahagún ◽  
José L Rodríguez ◽  
Joel N Pérez
Keyword(s):  
Solanum Tuberosum ◽  
Solanum Tuberosum L ◽  
In Vitro Regeneration ◽  
Potato Cultivars ◽  
Pcr Analysis ◽  
Nptii Gene ◽  
Histochemical Assay ◽  
Gus Histochemical Assay ◽  
Binary Plasmid

Prior to Agrobacterium-mediated genetic transformation in vitro regeneration protocol was established for three potato cultivars (Alfa, Cambray Rosa Morelos and Atlantic) grown in Mexico using leaf, node and internodal explants. Regeneration protocol was developed with or without the intervention of callus. Two potato cultivars, namely, Cambray Rosa Morelos and Alpha were transformed using Agrobacterium tumefaciens strain LBA4404 harboring binary plasmid pBI121 containing the GUS and nptII genes. GUS histochemical assay and PCR analysis were conducted on rooted shoots grown in media without hormones but supplemented with antibiotics. Transformed shoots tested positive through GUS histochemical assay and integration of nptII gene was confirmed by PCR analysis DOI: http://dx.doi.org/10.3329/ptcb.v22i2.14193 Plant Tissue Cult. & Biotech. 22(2): 93-105, 2012 (December)

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