Pectinase production from Aspergillus niger IBT-7 using solid state fermentation

2018 ◽  
Vol 47 (3) ◽  
pp. 473-478 ◽  
Author(s):  
Roheena Abdullah ◽  
Iqra Farooq ◽  
Afshan Kaleem ◽  
Mehwish Iqtedar ◽  
Tehreema Iftikhar
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Fruits And Vegetables ◽  
Nitrogen Sources ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Local Soil ◽  
Microscopic Studies ◽  
Pectinase Production

Different fungal strains were isolated from the local soil, fruits and vegetables on the basis of pectin hydrolysis. All the isolated strains were identified through microscopic studies and screened for pectinase production using solid state fermentation. The fungal strain identified as Aspergillus niger IBT-7 showed the highest pectinase production. The selected strain was further subjected to optimization through different physical and nutritional parameters to enhance the production of pectinase. Amongst seven different media tested M1 containing rice bran, moistened with Czapek’s nutrient medium showed the highest pectinase production. During optimization maximum pectinase production was achieved after 72 hrs of incubation at 30 ml of moisture content, pH 5.0 and 30°C. Xylose (1.5%) and yeast extract (1%) proved to be best supplemented carbon and nitrogen sources, respectively which gave the highest pectinase production (39.1 U/ml/min).

scholarly journals Evaluation of agricultural byproducts for the production of betaglucosidase by Aspergillus niger MBT-2 using solid state fermentation

2020 ◽  
Vol 49 (1) ◽  
pp. 135-140
Author(s):  
Roheena Abdullah ◽  
Maria Hanif ◽  
Afshan Kaleem ◽  
Mehwish Iqtedar ◽  
Kinza Nisar ◽  
...  
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Low Cost ◽  
Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Enhanced Production ◽  
Enzyme Productivity ◽  
Valuable Product ◽  
By Products

The experiment was conducted to isolate and screen fungal strain and optimization of solid-state fermentation conditions for enhanced production of β-glucosidase. Different fungal cultures were isolated and screened for β-glucosidase production. The physicochemical and nutritional parameters were optimized for enhanced production of β-glucosidase from higher producer. Among all the isolates the isolate which exhibited highest β-glucosidase potential was identified and assigned the code as Aspergillus niger MBT-2. The optimum β-glucosidase production was obtained in M5 medium containing wheat bran after 72 hrs of incubation at 40°C, pH 6 and 20 ml of moisture contents. In addition to this 2% fructose and 2% yeast extract proved to be best carbon and nitrogen sources, respectively and gave maximal enzyme productivity. The exploitation of agricultural by products as a substrate reduced the production cost of enzyme and makes the process economical. The Aspergillus niger MBT-2 has promising potential of bioconversion of low-cost material into valuable product like β-glucosidase.

scholarly journals PECTINASE PRODUCTION BY ASPERGILLUS NIGER IOC 4003 BY SOLID-STATE FERMENTATION USING CAJA DEPULPING RESIDUE

2018 ◽  
Author(s):  
J. C. ROCHA ◽  
J. S. ARAÚJO ◽  
W. K. V. PAIVA ◽  
C. R. R. FERREIRA ◽  
M. G. F. SILVA ◽  
...  
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Pectinase Production

scholarly journals Nutritional Requirements for the Improvement of Growth and Sporulation of Several Strains ofMonascus purpureuson Solid State Cultivation

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Zahra Ajdari ◽  
Afshin Ebrahimpour ◽  
Musaalbakri Abdul Manan ◽  
Muhajir Hamid ◽  
Rosfarizan Mohamad ◽  
...  
Keyword(s):  
Solid State ◽  
Medium Optimization ◽  
Cell Mass ◽  
Basal Medium ◽  
Nitrogen Sources ◽  
Nutritional Requirements ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
The Media ◽  
Sporulation Rate

This paper describes the nutritional requirements for the improvement of growth and sporulation of several strains ofMonascus purpureuson solid state cultivation. The findings revealed that glucose enhanced growth of allM. purpureusstrains tested but inhibited the sporulation rate. On the other hand, sucrose induced sporulation but inhibited production of cell mass. A combination of glucose and sucrose greatly enhanced sporulation and cell mass production ofM. purpureus. Although growth and sporulation rate were related to the ratio of carbon to nitrogen (C/N ratio), the types and concentrations of carbon and nitrogen sources also greatly influenced the growth kinetics. Among the media tested, Hiroi-PDA medium was the most preferred medium for allM. purpureusstrains tested for the enhancement of radial growth rate, sporulation, and cell production. Hence, Hiroi-PDA could be suggested as the generic basal medium for the cultivation ofM. purpureus. However, individual medium optimization is required for significant enhancement in growth and sporulation of each strain ofM. purpureus.

scholarly journals Production of Xylanase from Arthrobacter sp. MTCC 6915 Using Saw Dust As Substrate under Solid State Fermentation

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Sevanan Murugan ◽  
Donna Arnold ◽  
Uma Devi Pongiya ◽  
P.M. Narayanan
Keyword(s):  
Enzyme Activity ◽  
Solid State ◽  
Incubation Temperature ◽  
Nitrogen Sources ◽  
Optimal Temperature ◽  
Carbon And Nitrogen Sources ◽  
Xylanase Production ◽  
Carbon And Nitrogen ◽  
Saw Dust ◽  
Production Temperature

Saw dust was used as substrate for xylanase production from Arthrobacter sp. MTCC 6915. The study of period of incubation, temperature, pH, carbon, and nitrogen sources for xylanase production was optimized. Xylanase production was found to be optimum at an incubation period of 96 hrs (117.0 U/mL), temperature 30°C (105.0 U/mL), and pH 9.0 (102.9 U/mL). The results showed that the xylanase production was found to be higher in the presence of carboxymethylcellulose (176.4 U/mL) and dextrose (126.0 U/mL). It was also observed that peptone (170.1 U/mL) and beef extract (161.7 U/mL) supported maximum xylanase production.The enzyme was characterized and found to be fairly active at pH 9 (764.4 U/mL) and temperature 60°C (819 U/mL). Even in the present study, a major difference in the production temperature (30°C) and optimal temperature (60°C) of the enzyme activity was observed. However, the pH of the production media and the enzyme activity were found to be the same (pH 9).

Effect of Carbon and Nitrogen Sources on Xylanase Production by Mutant Strain of Aspergillus niger GCBMX-45

2002 ◽  
Vol 2 (2) ◽  
pp. 143-144 ◽  
Author(s):  
Ikram-ul-Haq . ◽  
Ayesha Khan . ◽  
Waseem Ahmad Butt . ◽  
Sikander Ali . ◽  
M. A. Qadeer .
Keyword(s):  
Aspergillus Niger ◽  
Mutant Strain ◽  
Nitrogen Sources ◽  
Carbon And Nitrogen Sources ◽  
Xylanase Production ◽  
Carbon And Nitrogen
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