scholarly journals Production of Xylanase from Arthrobacter sp. MTCC 6915 Using Saw Dust As Substrate under Solid State Fermentation

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Sevanan Murugan ◽  
Donna Arnold ◽  
Uma Devi Pongiya ◽  
P.M. Narayanan
Keyword(s):  
Enzyme Activity ◽  
Solid State ◽  
Incubation Temperature ◽  
Nitrogen Sources ◽  
Optimal Temperature ◽  
Carbon And Nitrogen Sources ◽  
Xylanase Production ◽  
Carbon And Nitrogen ◽  
Saw Dust ◽  
Production Temperature

Saw dust was used as substrate for xylanase production from Arthrobacter sp. MTCC 6915. The study of period of incubation, temperature, pH, carbon, and nitrogen sources for xylanase production was optimized. Xylanase production was found to be optimum at an incubation period of 96 hrs (117.0 U/mL), temperature 30°C (105.0 U/mL), and pH 9.0 (102.9 U/mL). The results showed that the xylanase production was found to be higher in the presence of carboxymethylcellulose (176.4 U/mL) and dextrose (126.0 U/mL). It was also observed that peptone (170.1 U/mL) and beef extract (161.7 U/mL) supported maximum xylanase production.The enzyme was characterized and found to be fairly active at pH 9 (764.4 U/mL) and temperature 60°C (819 U/mL). Even in the present study, a major difference in the production temperature (30°C) and optimal temperature (60°C) of the enzyme activity was observed. However, the pH of the production media and the enzyme activity were found to be the same (pH 9).

scholarly journals Optimum Conditions for Phenol Oxidase (Laccase) Production from Azotobacter chroococcum by Submerged Fermentation

2017 ◽  
Vol 11 (1) ◽  
pp. 13-18
Author(s):  
Ali J. R. AL-Sa'ady ◽  
Lamees M.R. Abbas ◽  
Hutaf A.A. ALsalim
Keyword(s):  
Incubation Period ◽  
Incubation Temperature ◽  
Nitrogen Sources ◽  
Phenol Oxidase ◽  
Azotobacter Chroococcum ◽  
Laccase Production ◽  
Optimum Conditions ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Production Conditions

Phenol oxidases (laccase), copper-containing oxidase enzymes, are found in many plants, fungi and microorganisms. Many factors that influence laccase production from Azotobacter chroococcum are determined in this study, these factors are: carbon source, nitrogen source, pH, incubation temperature and incubation period. The results showed that the best carbon and nitrogen sources for laccase production are sucrose and yeast extract respectively, and the best factors for laccase production conditions are pH 7.0 with an incubation period of 6 days at temperature 30 Cᵒ.

scholarly journals Nutritional Requirements for the Improvement of Growth and Sporulation of Several Strains ofMonascus purpureuson Solid State Cultivation

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Zahra Ajdari ◽  
Afshin Ebrahimpour ◽  
Musaalbakri Abdul Manan ◽  
Muhajir Hamid ◽  
Rosfarizan Mohamad ◽  
...  
Keyword(s):  
Solid State ◽  
Medium Optimization ◽  
Cell Mass ◽  
Basal Medium ◽  
Nitrogen Sources ◽  
Nutritional Requirements ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
The Media ◽  
Sporulation Rate

This paper describes the nutritional requirements for the improvement of growth and sporulation of several strains ofMonascus purpureuson solid state cultivation. The findings revealed that glucose enhanced growth of allM. purpureusstrains tested but inhibited the sporulation rate. On the other hand, sucrose induced sporulation but inhibited production of cell mass. A combination of glucose and sucrose greatly enhanced sporulation and cell mass production ofM. purpureus. Although growth and sporulation rate were related to the ratio of carbon to nitrogen (C/N ratio), the types and concentrations of carbon and nitrogen sources also greatly influenced the growth kinetics. Among the media tested, Hiroi-PDA medium was the most preferred medium for allM. purpureusstrains tested for the enhancement of radial growth rate, sporulation, and cell production. Hence, Hiroi-PDA could be suggested as the generic basal medium for the cultivation ofM. purpureus. However, individual medium optimization is required for significant enhancement in growth and sporulation of each strain ofM. purpureus.

Optimum the Fermentation Condition to Produce the Polysaccharide of Hericium erinaceus with Tofu Whey

2014 ◽  
Vol 998-999 ◽  
pp. 275-278
Author(s):  
Zuo Fa Zhang ◽  
Guo Ying Lv ◽  
Xue Jiang ◽  
Lei Fa Fan
Keyword(s):  
Incubation Temperature ◽  
Environmental Parameters ◽  
Nitrogen Sources ◽  
Fermentation Condition ◽  
Cultivation Medium ◽  
Carbon And Nitrogen Sources ◽  
Hericium Erinaceus ◽  
Carbon And Nitrogen ◽  
Potato Extract ◽  
Optimum Production

The present study was to evaluate the effects of certain nutrients and environmental parameters on growth and production of polysaccharide of Hericium erinaceus with tofu whey. The effect of different carbon and nitrogen sources, temperature, pH and incubation period on the production of polysaccharide in flask cultures by H. erinaceus was investigated. Glucose and potato extract were found to be the most suitable carbon and nitrogen source, respectively. Optimum production of polysaccharide required glucose in the cultivation medium with a concentration of 8.0 g/L. On the other hand, the highest production of polysaccharide was obtained in a cultivation medium containing 12.0 g/L of potato extract. Furthermore, the incubation temperature at 27 °C, pH 5.5, rotation rate 180 and seven days of the incubation were found to be the optimum for maximum production of polysaccharide of H. erinaceus.

Effect of Carbon and Nitrogen Sources on Xylanase Production by Mutant Strain of Aspergillus niger GCBMX-45

2002 ◽  
Vol 2 (2) ◽  
pp. 143-144 ◽  
Author(s):  
Ikram-ul-Haq . ◽  
Ayesha Khan . ◽  
Waseem Ahmad Butt . ◽  
Sikander Ali . ◽  
M. A. Qadeer .
Keyword(s):  
Aspergillus Niger ◽  
Mutant Strain ◽  
Nitrogen Sources ◽  
Carbon And Nitrogen Sources ◽  
Xylanase Production ◽  
Carbon And Nitrogen

Production and Optimization of Cellulase Activity of Thermomonospora Viridis Isolated From Rice Straw

2021 ◽  
Vol 50 (2) ◽  
pp. 395-404
Author(s):  
Faozia Faleha Sadida ◽  
Ma Manchur
Keyword(s):  
Enzyme Activity ◽  
Metal Ions ◽  
Rice Straw ◽  
Cellulase Activity ◽  
Nitrogen Sources ◽  
Cellulase Production ◽  
Tetraacetic Acid ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Optimum Ph

A highly cellulolytic actinomycete SR1 was locally isolated from rice straw and provisionally identified as Thermomonospora viridis. Optimum pH, temperature, carbon and nitrogen sources for its cellulase production were 6.5, 35°C, Carboxymethyl cellulase (CMC) and yeast extract, respectively whereas those of cellulase activity were 7.5, 40°C, CMC and peptone respectively. The effects of various metal ions and different reductant and inhibitors on its cellulase activity were investigated. Univalent Ag+ was found to decrease the enzyme activity whereas increased by bivalent Mg2+. Ethylene diamine tetraacetic acid (EDTA) caused remarkable decrease of cellulase activity but β-Mercaptoethanol stimulated its cellulase activity. Bangladesh J. Bot. 50(2): 395-404, 2021 (June)

Pectinase production from Aspergillus niger IBT-7 using solid state fermentation

2018 ◽  
Vol 47 (3) ◽  
pp. 473-478 ◽  
Author(s):  
Roheena Abdullah ◽  
Iqra Farooq ◽  
Afshan Kaleem ◽  
Mehwish Iqtedar ◽  
Tehreema Iftikhar
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Fruits And Vegetables ◽  
Nitrogen Sources ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Local Soil ◽  
Microscopic Studies ◽  
Pectinase Production

Different fungal strains were isolated from the local soil, fruits and vegetables on the basis of pectin hydrolysis. All the isolated strains were identified through microscopic studies and screened for pectinase production using solid state fermentation. The fungal strain identified as Aspergillus niger IBT-7 showed the highest pectinase production. The selected strain was further subjected to optimization through different physical and nutritional parameters to enhance the production of pectinase. Amongst seven different media tested M1 containing rice bran, moistened with Czapek’s nutrient medium showed the highest pectinase production. During optimization maximum pectinase production was achieved after 72 hrs of incubation at 30 ml of moisture content, pH 5.0 and 30°C. Xylose (1.5%) and yeast extract (1%) proved to be best supplemented carbon and nitrogen sources, respectively which gave the highest pectinase production (39.1 U/ml/min).

Sign in / Sign up

Export Citation Format

Share Document