Adventitious shoot regeneration from leaf and stem explants of Salix matsudana Koidz

Currently, the leaf and stem as explants for rapid propagation in vitro remained unknown in Salix matsudana. Multiple shoots were regenerated from leaf and stem (stem apex and stem with axil). The optimum medium for callus regeneration and shoots induction from leaf was on WPM medium containing 6.0 mg/l zeatin (Z) and 0.75 mg/l NAA. The optimum medium of shoot induction from stem apex was on MS medium supplemented with 8.0 mg/l Z and 1.0 mg/l NAA, and from stem with axil was on MS with 8.0 mg/l Z and 0.5 mg/l NAA. Rooting of regenerated shoots was obtained on the same medium supplemented with 1.0 mg/l activated charcoal.

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Investigation of adventitious shoot regeneration from in vitro stem explants of Melissa officinalis L.

Journal of Medicinal Plants Research ◽

10.5897/jmpr12.055 ◽

2012 ◽

Vol 6 (16) ◽

Author(s):

Hojat Moradkhani

Keyword(s):

Shoot Regeneration ◽

Adventitious Shoot ◽

Stem Explants ◽

Melissa Officinalis ◽

Adventitious Shoot Regeneration

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Adventitious shoot regeneration from in vitro stem explants of Phellodendron amurense

AFRICAN JOURNAL OF BIOTECHNOLOGY ◽

10.5897/ajb11.1569 ◽

2011 ◽

Vol 10 (64) ◽

pp. 14024-14028 ◽

Cited By ~ 1

Author(s):

Wang Hui Mei ◽

Chen Ya Jie ◽

Yu Hai Di ◽

Zu Yuan Gang

Keyword(s):

Shoot Regeneration ◽

Adventitious Shoot ◽

Stem Explants ◽

Adventitious Shoot Regeneration ◽

Phellodendron Amurense

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A Two-step Procedure for Adventitious Shoot Regeneration from in vitro-derived Lingonberry Leaves: Shoot Induction with TDZ and Shoot Elongation Using Zeatin

HortScience ◽

10.21273/hortsci.40.1.189 ◽

2005 ◽

Vol 40 (1) ◽

pp. 189-192 ◽

Cited By ~ 24

Author(s):

Samir C. Debnath

Keyword(s):

Survival Rate ◽

Shoot Regeneration ◽

Adventitious Shoot ◽

Shoot Elongation ◽

Adventitious Bud ◽

Shoot Induction ◽

Adventitious Shoot Regeneration ◽

Explant Orientation ◽

Step Procedure

The effects of TDZ (0, 0.1, 1, 5 and 10 μm) and explant orientation on adventitious shoot regeneration of `Erntedank' lingonberry were studied. Moderate concentration (1 to 5 μm) of TDZ supported bud and shoot regeneration, but strongly inhibited shoot elongation. TDZ initiated cultures were transferred to medium containing 1-2 μm zeatin and produced usable shoots after one additional subculture. Adventitious bud and shoot regeneration was greatly influenced by explant orientation. Elongated shoots were rooted on a 2 peat: 1 perlite (v/v) medium, and the plantlets were acclimatized and eventually established in the greenhouse with 80% to 90% survival rate.

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Rejuvenation of chicory and lettuce plants following phase change in tissue culture

BMC Biotechnology ◽

10.1186/s12896-019-0557-z ◽

2019 ◽

Vol 19 (1) ◽

Cited By ~ 1

Author(s):

Anthony J. Conner ◽

Helen Searle ◽

Jeanne M. E. Jacobs

Keyword(s):

Tissue Culture ◽

Shoot Regeneration ◽

Seed Set ◽

Genetic Manipulation ◽

Adventitious Shoot ◽

In Vitro Flowering ◽

Phase Changes ◽

Adventitious Shoot Regeneration ◽

Cell And Tissue Culture

Abstract Background A frequent problem associated with the tissue culture of Compositae species such as chicory (Cichorium intybus L.) and lettuce (Lactuca sativa L.) is the premature bolting to in vitro flowering of regenerated plants. Plants exhibiting such phase changes have poor survival and poor seed set upon transfer from tissue culture to greenhouse conditions. This can result in the loss of valuable plant lines following applications of cell and tissue culture for genetic manipulation. Results This study demonstrates that chicory and lettuce plants exhibiting stable in vitro flowering can be rejuvenated by a further cycle of adventitious shoot regeneration from cauline leaves. The resulting rejuvenated plants exhibit substantially improved performance following transfer to greenhouse conditions, with increased frequency of plant survival, a doubling of the frequency of plants that flowered, and substantially increased seed production. Conclusion As soon as in vitro flowering is observed in unique highly-valued chicory and lettuce lines, a further cycle of adventitious shoot regeneration from cauline leaves should be implemented to induce rejuvenation. This re-establishes a juvenile phase accompanied by in vitro rosette formation, resulting in substantially improved survival, flowering and seed set in a greenhouse, thereby ensuring the recovery of future generations from lines genetically manipulated in cell and tissue culture.

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In vitro Propagation of Polygonum hydropiper L. from Shoot Tips

Plant Tissue Culture and Biotechnology ◽

10.3329/ptcb.v20i1.5970 ◽

2010 ◽

Vol 20 (1) ◽

pp. 73-79 ◽

Cited By ~ 2

Author(s):

M. F. Hasan ◽

B. Sikdar

Keyword(s):

Plant Regeneration ◽

In Vitro Propagation ◽

Multiple Shoots ◽

Multiple Shoot ◽

Shoot Tips ◽

Root Induction ◽

Shoot Induction ◽

Multiple Shoot Induction ◽

Polygonum Hydropiper

An efficient protocol for plant regeneration through multiple shoots induction from shoot tips of Polygonum hydropiper (L.) was established. The highest percentage (96.6) of multiple shoot induction and number of shoots (9.0) per culture were found on MS supplemented with 2.0 mg/l Kn. The induced shoots were excised and inoculated on to MS contains different concentrations of IBA or NAA for rooting. The highest percentage (90.0) of root induction and the highest number of roots per shoot (12.0) was found on MS having 1.0 mg/l IBA. Well rooted plantlets were acclimated properly and transplanted in the soil under natural condition, where cent per cent plantlets survived and grew successfully. Key words: Polygonum hydropiper, Shoot tips, In vitro propagation D.O.I. 10.3329/ptcb.v20i1.5970 Plant Tissue Cult. & Biotech. 20(1): 73-79, 2010 (June)

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