scholarly journals Characterization of microorganisms from Sitakunda hot spring

2013 ◽  
pp. 107-117
Author(s):  
MZ Hossain ◽  
MN Anwar
Keyword(s):  
Bacillus Megaterium ◽  
Hot Spring ◽  
Winter Season ◽  
Bacillus Coagulans ◽  
Total Alkalinity ◽  
Bacillus Laterosporus ◽  
Bacillus Popilliae ◽  
Recorded Temperature ◽  
Direct Microscopic Observation

Water, leaf, stone and sand samples from Sitakunda hot spring were studied and the microorganisms isolated from these samples were thoroughly characterized. The pH of the collected samples was found to range from 8.5 to 8.9 and the recorded temperature varied from 26.1 to 29.8 ºC during winter season. BOD of the collected water samples ranged from 10 to 92 mg/L, while COD was almost zero. The total alkalinity and chlorine content ranged from 12 to 20 mg/L and 13.9 to 18.3 gm/L respectively. Quantitative enumeration of bacterial population showed the range of 8.0×102 to 3.8×105 cfu/ml or gm sample. All the 9 selected isolates were identified as Bacillus coagulans (S1B4), Bacillus laterosporus (SB18), Bacillus megaterium (S2B11), Bacillus popilliae (S4P1), Bacillus firmus (S5B1), Citrobacter intermedius (S4B17), Listeria monocytogenes (S1B1), Listeria denitrificans (S2B) and Pseudomonas mendocina (S4P3). Bacillus laterosporus, Bacillus megaterium and Listeria denitrificans showed better growth in presence of methane gas in nutrient broth. By direct microscopic observation 7 genera of cyanobacteria (Arthrospria, Microcoleus, Oscillatoria, Schyzothrix, Scytonema, Nostoc and Gloeocapsa) and 4 genera of diatoms (Gomphonema, Melosira, Navicula and Pinnularia) were detected in the water sample DOI: http://dx.doi.org/10.3329/cujbs.v4i1.13394 The Chittagong Univ. J. B. Sci.,Vol. 4(1&2):107-117, 2009

scholarly journals The thermostable 4,6-α-glucanotransferase of Bacillus coagulans DSM 1 synthesizes isomaltooligosaccharides

Amylase ◽  
2021 ◽  
Vol 5 (1) ◽  
pp. 13-22
Author(s):  
Gang Xiang ◽  
Piet L. Buwalda ◽  
Marc J.E.C van der Maarel ◽  
Hans Leemhuis
Keyword(s):  
Bacillus Coagulans ◽  
Glycoside Hydrolase Family ◽  
Rat Intestine ◽  
Glycaemic Response ◽  
Food Ingredient ◽  
Starch Conversion ◽  
Identification And Characterization ◽  
Hydrolase Family

Abstract The 4,6-α-glucanotransferases of the glycoside hydrolase family 70 can convert starch into isomaltooligosaccharides (IMOs). However, no thermostable 4,6-α-glucanotransferases have been reported to date, limiting their applicability in the starch conversion industry. Here we report the identification and characterization of a thermostable 4,6-α-glucanotransferase from Bacillus coagulans DSM 1. The gene was cloned and the recombinant protein, called BcGtfC, was produced in Escherichia coli. BcGtfC is stable up to 66 °C in the presence of substrate. It converts debranched starch into an IMO product with a high percentage of α-1,6-glycosidic linkages and a relatively high molecular weight compared to commercially available IMOs. Importantly, the product is only partly and very slowly digested by rat intestine powder, suggesting that the IMO will provide a low glycaemic response in vivo when applied as food ingredient. Thus, BcGtfC is a thermostable 4,6-α-glucanotransferase suitable for the industrial production of slowly digestible IMOs from starch.

scholarly journals Isolation and characterization of two virulent Aeromonads associated with haemorrhagic septicaemia and tail-rot disease in farmed climbing perch Anabas testudineus

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Abhishek Mazumder ◽  
Hrishikesh Choudhury ◽  
Abhinit Dey ◽  
Dandadhar Sarma
Keyword(s):  
Natural Infection ◽  
Winter Season ◽  
Pcr Amplification ◽  
Body Parts ◽  
Anabas Testudineus ◽  
Climbing Perch ◽  
Isolation And Characterization ◽  
Biochemical Characterisation ◽  
Rot Disease

AbstractDiseased Anabas testudineus exhibiting signs of tail-rot and ulcerations on body were collected from a fish farm in Assam, India during the winter season (November 2018 to January 2019). Swabs from the infected body parts were streaked on sterilized nutrient agar. Two dominant bacterial colonies were obtained, which were then isolated and labelled as AM-31 and AM-05. Standard biochemical characterisation and 16S rRNA and rpoB gene sequencing identified AM-31 isolate as Aeromonas hydrophila and AM-05 as Aeromonas jandaei. Symptoms similar to that of natural infection were observed on re-infecting both bacteria to disease-free A. testudineus, which confirmed their virulence. LC50 was determined at 1.3 × 104 (A. hydrophila) and 2.5 × 104 (A. jandaei) CFU per fish in intraperitoneal injection. Further, PCR amplification of specific genes responsible for virulence (aerolysin and enterotoxin) confirmed pathogenicity of both bacteria. Histopathology of kidney and liver in the experimentally-infected fishes revealed haemorrhage, tubular degeneration and vacuolation. Antibiotic profiles were also assessed for both bacteria. To the best of our knowledge, the present work is a first report on the mortality of farmed climbing perch naturally-infected by A. hydrophila as well as A. jandaei, with no records of pathogenicity of the latter in this fish.

Characterization of a thermostable manganese-containing superoxide dismutase from inshore hot spring thermophile Thermus sp. JM1

2011 ◽  
Vol 30 (6) ◽  
pp. 95-103 ◽  
Author(s):  
Yanbing Zhu ◽  
Hebin Li ◽  
Xuqin Zhang ◽  
Chunyan Zhang ◽  
Jionghua Xiang ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Hot Spring
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