BAKTERI ASAL FUNGI MIKORIZA ARBUSKULA YANG BERPOTENSI SEBAGAI AGEN DEKOMPOSER SAMPAH ORGANIK

Penelitian ini bertujuan untuk mengetahui bakteri asal Spora Fungi Mikoriza Arbuskula (FMA) yang memiliki kemampuan menghasilkan enzim hidrolitik selulase dan protease guna dijadikan sebagai potensi agen dekomposer sampah organik. Metode penelitian menggunakan metode isolasi spora FMA, Isolasi Bakteri dan Pengujian Enzimatik. Hasil penelitian menunujukan bahwa terdapat 6 jenis bakteri mampu menghasilkan enzim selulase dan 5 jenis bakteri menghasilkan enzim protease. 3 jenis bakteri memiliki kemampuan menghasilkan enzim selulase sekaligus protease tertinggi yaitu Bacillus cereus, Bacillus laterosporus dan Bacillus pasteurii. Ke-3 jenis bakteri ini diharapkan dapat dikembangkan sebagai agen dekomposer sampah organik.

The emulsifying effect of biosurfactants produced by food spoilage organisms in Nigeria

Food spoilage organisms were isolated using standard procedures on Nutrient Agar, Cetrimide Agar and Pseudomonas Agar Base (supplemented with CFC). The samples were categorized as animal products (raw fish, egg, raw chicken, corned beef, pasteurized milk) and plant products (vegetable salad, water leaf (Talinium triangulare), boiled rice, tomatoes and pumpkin leaf (Teifairia occidentalis).They were characterised as Pseudomonas putida, Pseudomonas aeruginosa, Pseudomonas stutzeri, Burkholderia pseudomallei, Serratia rubidaea, Corynebacterium pilosum, Bacillus subtilis, Bacillus mycoides, Bacillus laterosporus, Bacillus laterosporus, Serratia marcescens, Bacillus cereus, Bacillus macerans, Alcaligenes faecalis and Alcaligenes eutrophus. Preliminary screening for biosurfactant production was done using red blood haemolysis test and confirmed by slide test, drop collapse and oil spreading assay. The biosurfactant produced was purified using acetone and the composition determined initially using Molisch’s test, thin layer chromatography and gas chromatography mass spectrometry. The components were found to be ethanol, amino acids, butoxyacetic acid, hexadecanoic acid, oleic acid, lauryl peroxide, octadecanoic acid and phthalic acid. The producing organisms grew readily on several hydrocarbons such as crude oil, diesel oil and aviation fuel when used as sole carbon sources. The purified biosurfactants produced were able to cause emulsification of kerosene (19.71-27.14%) as well as vegetable oil (16.91-28.12%) based on the emulsification index. This result suggests that the isolates can be an asset and further work can exploit their optimal potential in industries.

PENGARUH FERMENTASI TEPUNG DAUN LAMTORO (Leucaena leucocephala) DENGAN Bacillus laterosporus TERHADAP ENERGI METABOLISME, KECERNAAN SERAT KASAR DAN RETENSI NITROGEN PADA BROILER

Fermentasi tepung daun lamtoro dengan bacillus laterosporus sebelum dan sesudah fermentasi terhadap metabolisme energi, kecernaan serat kasar dan retensi nirtogen dengan menggunakan uji T. Fermentasi tepung lamtoro dapat meningkatkan metaboliosme energi , daya cerna serat kasar dan retensi nitrogen dinading sebelum fermentasi

Study on the Effects of 8 Strains on Growth Promotion and Disease Resistance of Tomato (Solanum lycopersicum)

The pot experiment was carried out to study the effects of eight functional strains on growth promotion of tomato and resistance to tomato bacterial wilt. The results indicated that the two strains both Paenibacillus polymyxa and Bacillus megaterium could significantly increase the plant height of tomato; the two strains of Paenibacillus azotoformans and Bacillus laterosporus could significantly improve the stem diameter of tomato; the three strains of Paenibacillus polymyxa, Bacillus laterosporus and Paenibacillus azotoformans could significantly increase the average leaf area and chlorophyll contents of Tomato; the four strains of Bacillus subtilis, Bacillus licheniformis, Paenibacillus polymyxa and Streptomyces microflavus could obviously inhibit occurrence of tomato bacterial wilt. All above results indicated that Paenibacillus polymyxa could not only significantly increase the height, the average leaf area and chlorophyll content of tomato plant, but also could obviously reduce the incidence of tomato bacterial wilt. So this strain can be used as one of core strains to construct multifunctional composite inoculants.

Characterization of microorganisms from Sitakunda hot spring

Water, leaf, stone and sand samples from Sitakunda hot spring were studied and the microorganisms isolated from these samples were thoroughly characterized. The pH of the collected samples was found to range from 8.5 to 8.9 and the recorded temperature varied from 26.1 to 29.8 ºC during winter season. BOD of the collected water samples ranged from 10 to 92 mg/L, while COD was almost zero. The total alkalinity and chlorine content ranged from 12 to 20 mg/L and 13.9 to 18.3 gm/L respectively. Quantitative enumeration of bacterial population showed the range of 8.0×102 to 3.8×105 cfu/ml or gm sample. All the 9 selected isolates were identified as Bacillus coagulans (S1B4), Bacillus laterosporus (SB18), Bacillus megaterium (S2B11), Bacillus popilliae (S4P1), Bacillus firmus (S5B1), Citrobacter intermedius (S4B17), Listeria monocytogenes (S1B1), Listeria denitrificans (S2B) and Pseudomonas mendocina (S4P3). Bacillus laterosporus, Bacillus megaterium and Listeria denitrificans showed better growth in presence of methane gas in nutrient broth. By direct microscopic observation 7 genera of cyanobacteria (Arthrospria, Microcoleus, Oscillatoria, Schyzothrix, Scytonema, Nostoc and Gloeocapsa) and 4 genera of diatoms (Gomphonema, Melosira, Navicula and Pinnularia) were detected in the water sample DOI: http://dx.doi.org/10.3329/cujbs.v4i1.13394 The Chittagong Univ. J. B. Sci.,Vol. 4(1&2):107-117, 2009