scholarly journals In Vitro Propagation of Pumpkin and Ash Gourd through Nodal Segments

1970 ◽  
Vol 16 ◽  
pp. 67-71 ◽  
Author(s):  
ME Haque ◽  
MAR Sarkar ◽  
MA Mahmud ◽  
D Rezwana ◽  
B Sikdar
Keyword(s):  
Shoot Multiplication ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Nodal Segments ◽  
Root Induction ◽  
Ms Medium ◽  
Shoot Bud ◽  
Benincasa Hispida ◽  
Ash Gourd

The present study was undertaken with a view to develop an efficient protocol for in vitro multiple shoot formation and subsequent root induction considering various cultural aspects using nodal segments of Cucurbita maxima and Benincasa hispida. The best hormone for shoot multiplication of pumpkin was BAP (2 mg/l), incase of ash-gourd BAP was 1.5 mg/l. For callus induction BAP+2,4-D was best combination for pumpkin and it was 2.0+0.1 mg/l when in ash gourd BAP+NAA was the best combination. IBA + ½MS medium were used for induction of shoot bud root. In both of pumpkin and ash gourd 1.5 mg/l IBA was found best for induction of roots. Key words: In vitro, MS medium, nodal segments, multiplication, rooting, pumpkin and ash gourd.   DOI:10.3329/jbs.v16i0.3743 J. bio-sci. 16: 67-71, 2008

scholarly journals Efficient Direct Organogenesis from Shoot Tips and Nodal Segments of Ash Gourd (Benincasa Hispida L.)

2014 ◽  
Vol 8 ◽  
pp. 17-20
Author(s):  
M Kausar ◽  
S Parvin ◽  
ME Haque ◽  
M Khalekuzzaman ◽  
B Sikdar ◽  
...  
Keyword(s):  
Shoot Formation ◽  
Multiple Shoot ◽  
Shoot Tips ◽  
Nodal Segments ◽  
Good Survival ◽  
Root Induction ◽  
Ms Medium ◽  
Multiple Shoot Formation ◽  
Benincasa Hispida ◽  
Ash Gourd

The effect of external application of phytohormone on explants viz., shoot tips and nodal segments of ash gourd (Benincasa hispida L.) was tested on the frequency of shoot and root induction. Shoot tips and nodal segments were cultured on MS medium supplemented with different concentrations and combinations of cytokinins (BAP, Kinetin), auxin (IBA, NAA) and gibberellic acid (GA3) for multiple shoot formation and root induction. The highest number (up to 90%) of multiple shoot formation was obtained from the shoot tips in MS medium fortified with 1.5 mg/l BAP + 0.2 mg/l GA3, where average number of shoots per culture was 5.55. In case of nodal segment, better response (up to 78%) for shoot multiplication was found in MS with 2.0 mg/l BAP + 0.2 mg/l GA3. The concentration of 1.0 mg/l IBA was found to be most effective for root initiation in microshoot developed from both types of explants. Rooted plantlets were acclimatatized and established in sandy soil with good survival rate DOI: http://dx.doi.org/10.3329/jles.v8i0.20135 J. Life Earth Sci., Vol. 8: 17-20, 2013

scholarly journals Establishment of in vitro regeneration protocol for Adhatoda vasica Nees

2016 ◽  
Vol 51 (1) ◽  
pp. 75-80 ◽  
Author(s):  
S Khan ◽  
S Akter ◽  
A Habib ◽  
TA Banu ◽  
M Islam ◽  
...  
Keyword(s):  
In Vitro Regeneration ◽  
Multiple Shoots ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Nodal Segments ◽  
Garden Soil ◽  
Root Induction ◽  
Ms Medium ◽  
Adhatoda Vasica

An in vitro regeneration protocol of Adhatoda vasica has been developed using excised nodal segments and juvenile leaves for multiple shoots regeneration directly or through callus induction. Explants were cultured on MS medium with different concentrations of IAA, NAA, BAP, GA3 and Kn singly or in combinations. MS medium supplemented with BAP (10.0 mg/l) was found best for multiple shoot formation, in which 93.33% explants produced multiple shoots. After two months, maximum number of multiple shoots were 10.6 ± 1.82, highest length of plantlets was 5.2 ± 2.20 cm. 100% calli formation were observed on MS medium supplemented with IAA (0.05 mg/l) + NAA (0.05 mg/l) + BAP (1.0 mg/l). Callus initiation started after 14 days and gave light green colored callus. Best callus mediated shoot regeneration was found on MS+10.0 mg/l BAP medium. Root induction of in vitro raised shoots was best on ½ MS + IBA (1.0 mg/l). Well rooted plantlets were transferred to plastic pots containing garden soil and compost in a ratio of 2:1 for hardening. The ultimate survival rate under natural condition was about 80%.Bangladesh J. Sci. Ind. Res. 51(1), 75-80, 2016

scholarly journals In vitro Shoot Cultures of Tupistra albiflora K. Larsen

2018 ◽  
Vol 17 (5) ◽  
pp. 405-411
Author(s):  
Jiraporn PALEE
Keyword(s):  
Agar Medium ◽  
Multiple Shoots ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Naphthalene Acetic Acid ◽  
Root Induction ◽  
Shoot Cultures ◽  
Ms Medium ◽  
In Vitro Shoots

To evaluate an efficient protocol for the micropropagation of Tupistra albiflora K. Larsen, the effects of N6-benzylaminopurine (BA) and naphthalene acetic acid (NAA) concentrations on multiple shoot and root induction were examined. In vitro shoots were used as the explant materials which were cultured on Murashige and Skoog (MS) agar medium supplemented with 0, 1, 2, 3 and 4 mg/L BA for 4 weeks to induce multiple shoots. It was found that the MS medium containing 3 mg/L BA induced 100 % shoot formation with the highest number of 3.2 shoots per explant (2.4-fold significantly higher than the control). For root induction, in vitro shoots were cultured on MS agar medium supplemented with 0, 1, 2, 3 and 4 mg/L NAA for 8 weeks. The results showed that the MS medium containing 1 mg/L NAA induced 100 % root formation with the highest number of 6.6 roots per explant (1.8-fold significantly higher than the control).

scholarly journals In vitro regeneration protocol of Rauvolfia serpentina L.

2018 ◽  
Vol 53 (2) ◽  
pp. 133-138 ◽  
Author(s):  
S Khan ◽  
TA Banu ◽  
S Akter ◽  
B Goswami ◽  
M Islam ◽  
...  
Keyword(s):  
In Vitro Regeneration ◽  
Leaf Explants ◽  
Multiple Shoot ◽  
Nodal Segments ◽  
Root Induction ◽  
Ms Medium ◽  
Regeneration System ◽  
Rauvolfia Serpentina ◽  
Number Of Shoots

An efficient in vitro regeneration system was developed for Rauvolfia serpentina L. through direct and indirect organogenesis from nodal and leaf explants. Among the different growth regulators, MS medium supplemented with 2.0 mg/l BAP, 0.5mg/l IAA and 0.02mg/l NAA found best for the multiple shoot formation from nodal segments. In this combination 98% explants produced multiple shoots and the average number of shoots per explants is 13∙4. The frequency of callus induction and multiple shoot induction from leaves was highest 88% in MS medium supplemented with 2.0 mg/l BAP, where mean number of shoots/explants was 12.5. The highest frequency of root induction (80%) and mean number of roots/plantlets (10) were obtained on half strength of MS medium containing 0.2 mg/l IBA. The rooted plantlets were transferred for hardening following acclimatization and finally were successfully established in the field.Bangladesh J. Sci. Ind. Res.53(2), 133-138, 2018

scholarly journals In Vitro Micropropagation of Orchid (Vanda tessellata L.) from Shoot Tip Explant

1970 ◽  
Vol 17 ◽  
pp. 139-144 ◽  
Author(s):  
MS Rahman ◽  
MF Hasan ◽  
R Das ◽  
MS Hossain ◽  
M Rahman
Keyword(s):  
Multiple Shoots ◽  
Shoot Elongation ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Shoot Tips ◽  
Shoot Tip ◽  
Root Induction ◽  
Ms Medium ◽  
Culture Techniques

Context: Orchid produces a huge number of minute seeds but the seeds can not germinate easily in nature due to the lack of endosperm in the seeds is an incompatibility barrier that limits its propagation in nature. Objectives: To develop in vitro culture techniques for quick propagation of Vanda tessellate, a commercially important orchid species. Materials and Methods: Shoot tips were used as experimental materials. The explants were surface sterilized and the shoot tips were excised. The isolated shoot tips were cultured in MS medium supplemented with different concentration and combinations of auxin and cytokinin. Results: The combination of 1.5 mgl-1 NAA and 1.0 mgl-1 BAP was proved to be the best medium formulation for multiple shoot formation as well as maximum shoot elongation. The single shoots were isolated from the multiple shoots and subcultured in MS medium having NAA and IBA individually and in combinations for root induction. Maximum root induction was obtained in MS agarified medium having 0.5 mgl-1NAA and 1.0 mgl-1IBA. The well rooted plantlets were hardened successfully in the potting mixture containing coconut husk, perlite, charcoal, brick pieces in the ratio of 2:1:1:1 and eventually established under natural condition.Conclusion: An efficient regeneration protocol for micropropagation in V. tessellata through shoot tip culture has been established.Key words: Shoot tip; micropropagation; orchid.DOI: 10.3329/jbs.v17i0.7122J. bio-sci. 17: 139-144, 2009

scholarly journals Optimization of growth regulators on in vitro propagation of Moringa stenopetala from shoot explants

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Alelegne Yeshamebel Adugna ◽  
Tileye Feyissa ◽  
Fikresilasie Samuel Tasew
Keyword(s):  
Shoot Multiplication ◽  
Multiple Shoot ◽  
Southern Ethiopia ◽  
Naphthalene Acetic Acid ◽  
Root Induction ◽  
Ms Medium ◽  
Multiple Shoot Induction ◽  
Moringa Stenopetala ◽  
Significant Difference

Abstract Background Moringa stenopetala belongs to the flowering family Moringaceae and genus Moringa. It is often referred to as the East African Moringa tree because it is native only to southern Ethiopia and northern Kenya. The expansion of its cultivation and utilization throughout the world especially in Africa is becoming important. For such expansion, the existing propagation method is limiting, so it needs a good propagation system to supply enough planting material with a uniform genotype. Therefore, the main objective of this study was to optimize an in vitro shoot multiplication protocol for M. stenopetala by using shoot tip as explants. Results Shoots were sterilized and cultured on Muraghige and Skoog (MS) medium for in vitro shoot initiation. For multiple shoot induction, the explants were cultured on MS medium supplemented with different concentrations of kinetin (0.5, 1.0, 1.5, 2.0, 2.5 mg/L) with Indole-3- butyric acid (IBA) or α -naphthalene acetic acid (NAA) (0.01, 0.1, 0.5 mg/L) and maintained at 25 ± 2 °C for four weeks. Rooting was achieved by culturing well developed shoots in half-strength MS medium containing IBA (0.1, 0.5, 1.0, 1.5, 2.0 mg/L), NAA (0.1, 0.5, 1.0, 1.5, 2.0 mg/L), and 0.5 mg/L IBA with NAA (0.1, 0.5, 1.0, 1.5, 2.0 mg/L). Statistical analysis revealed that there was a significant difference among all treatments applied in both shoot multiplication and rooting experiments. The maximum number of shoots per explant (3.43 ± 1.41) and 7.97 ± 4.18 leaves per explant were obtained on MS medium containing 0.5 mg/L kinetin with 0.01 mg/LNAA. The highest mean number of roots per shoot (1.63 ± 1.03) and mean root length (0.87 ± 1.22 cm) were obtained on MS medium containing 1.0 mg/LNAA and 0.1 mg/LIBA alone respectively. After acclimatization, 76% of plants were survived in the greenhouse. Conclusion In general, using NAA with kinetin for shoot multiplication was effective than kinetin with IBA. On the other hand, the application of 1.0 mg/L NAA alone and 1.0 mg/L NAA with 0.5 mg/L IBA were more effective for root induction.

scholarly journals CHIA SEED AS A SOURCE OF IN VITRO ESTABLISHMENT OF Salvia hispanica L. PLANTS

2020 ◽  
Vol 51 (3) ◽  
pp. 976-981
Author(s):  
Al- Dabagh & Salih
Keyword(s):  
Shoot Multiplication ◽  
Shoot Formation ◽  
Root Induction ◽  
Ms Medium ◽  
Salvia Hispanica ◽  
Chia Seed ◽  
Half Strength ◽  
Salvia Hispanica L ◽  
The Impact

 Technique of tissue culture for Chia (Salvia hispanica) micropropagation was achieved, this study investigated the impact of various concentrations of plant growth regulators on shoot multiplication and root induction with the Chia’s mature seed as a source explant. The highest percentage of shoot formation (80%), shoots number per explant(3.20) and shoot length(3.26 cm), were recorded on MS medium enriched with BAP(1.0 mgl-1) after eight weeks of seed culture. The optimal medium for the rhizogenesis was achieved on half strength MS medium fortified with 1.0 mgl-1 IBA after four weeks of culture, which had the highest rooting percentage (100%) with highest mean of roots number (5.6 roots per shoot) with (3.40 cm root length). The rooted plants were successfully adapted ex vitro with a survival rate of 85%.

scholarly journals Effect of Growth Regulators on In Vitro Micropropagation of Potato (Solanum tuberosum L.) Gudiene and Belete Varieties from Ethiopia

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Sunil Tulshiram Hajare ◽  
Nitin Mahendra Chauhan ◽  
Girum Kassa
Keyword(s):  
Solanum Tuberosum ◽  
Solanum Tuberosum L ◽  
Shoot Multiplication ◽  
Multiple Shoots ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Ms Medium ◽  
Shoot Initiation ◽  
Lateral Bud

Aim. Potato (Solanum tuberosum L.) is one of the important crops in Ethiopia which has a crucial role in nutritional security, poverty alleviation, and income generation. The aim of the present investigation is to develop an efficient in vitro propagation protocol for Belete and Gudiene potato varieties by using lateral bud as explants. Materials and Methods. Shoot initiation was achieved by inoculating buds on full-strength MS Murashige and Skoog medium (MS) fortified with variable concentrations of BAP and NAA. Basal MS was used as control throughout the experiment. Results. Results of our study showed that best shoot initiation was obtained on MS medium supplemented with 1.5 mg/l BAP + 3.0 mg/l NAA for Gudiene variety, whereas 1.0 mg/l BAP and 2.0 mg/l NAA produced more shoots in Belete variety. The initiated shoots increased two- to three-fold upon subculture on the MS medium fortified with varying concentrations of BAP and Kinetin. The highest numbers of multiple shoots were obtained in the MS medium containing 2.5 mg/l Kinetin. The combined effect of BAP and Kinetin did not produce any additional positive effect for shoot multiplication. Rooting percentage and number of roots/shoot were found best on the MS medium fortified with 1.0 mg/l IBA + 0.5 IAA. Conclusions. The variety Gudiene was found best for shoot initiation and root formation, while Belete variety proved its superiority for multiple shoot formation. A total number of 82.66% of plantlets were acclimatized under field conditions. This work indicates the practical applicability of plant tissue culture using lateral bud as explants is effective for micropropagation of potato in vitro.

scholarly journals Micropropagation of Ilex glabra (L.) A. Gray

HortScience ◽  
2010 ◽  
Vol 45 (5) ◽  
pp. 805-808 ◽  
Author(s):  
Youping Sun ◽  
Donglin Zhang ◽  
John Smagula
Keyword(s):  
Mass Production ◽  
Adventitious Roots ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Axillary Bud ◽  
Nodal Segments ◽  
Multiplication Rate ◽  
Ms Medium ◽  
Indolebutyric Acid

Nodal segments containing one axillary bud (1 to 1.5 cm) were disinfected using 10% bleach and were established on a Murashige and Skoog (MS) medium without hormones at 27 °C and with a 16-h photoperiod. The sprouted shoots (≈1.0 cm) were cultured on a MS medium supplemented with 6-benzylaminopurine (BAP), kinetin (KIN), or zeatin (ZT) at 2.3, 4.5, 9.1, or 18.2 μM. After 38 d, ZT and BAP significantly induced multiple shoot formation with multiplication rates of 4 to 6, whereas the multiplication rate of KIN was less than 2. Shoots cultured on ZT grew significantly taller than those on BAP and KIN. The height of the longest shoots treated with ZT was 4.6 cm, which was 1.6 to 2.2 times greater than those treated with BAP or KIN. To induce rooting, shoots (≈2 cm) were subcultured on one-fourth strength MS (1/4 MS) medium containing either 3-indolebutyric acid (IBA) or 1-naphthylacetic acid (NAA) at 2.6, 5.1, or 10.3 μM. Adventitious roots formed in vitro after 2 to 4 weeks. IBA at 10.3 μM produced the best rooting (100%) compared with other treatments after 38 d of culture. The average number of roots per shoot for IBA was ≈15, which was 1.6 to 3.1 times as many as that of other treatments. All rooted plantlets were then transplanted into a mix of peatmoss and perlite (1:1 v/v) and acclimatized in a mist system. Average plantlet survival was 73.6% after 35 d. After acclimatization, they were grown in a pot with Metro-mix under greenhouse conditions for 10 weeks where 95% of plants survived and grew up to 6.8 cm high. The micropropagation procedure, i.e., nodal segments containing one axillary bud proliferated on MS with 4.5 μM ZT followed by in vitro rooting on 1/4 MS plus 10.3 μM IBA, could be used for commercial mass production of new inkberry cultivars.

scholarly journals EXPLOITATION OF PLANT EXTRACTS (STEM AND ROOT) OF MICROPROPAGATED BACOPA MONNIERI : ANTIMYCOTIC POTENTIAL

2016 ◽  
Vol 2 (9) ◽  
Author(s):  
MRRIDULA DANGI NARWAL
Keyword(s):  
Antioxidant Activities ◽  
Average Length ◽  
Shoot Multiplication ◽  
Bacopa Monnieri ◽  
Nodal Segments ◽  
Root Induction ◽  
Ms Medium ◽  
In Vitro Production ◽  
Number Of Shoots

Bacopa monnieri is commonly called as brahmi or jal brahmi in India. Brahmi is a non-aromatic herb Brahmi is considered as the herb played a very important role in Ayurvedic medicine. It is found easily India, Australia, Europe, Africa, Asia. Bacopa monnieri (L) belongs to the family Scrophulariaceae is an amphibious plant of tropical and normally found growing on the banks of the rivers and lakes. It is commonly called as brahmi or jal brahmi in India. Brahmi is considered as the main rejuvenating herb played a very important role in Ayurvedic therapies. It also has anti-inflammatory, analgesic, antipyretic, epilepsy, anticancer, antioxidant activities and recently antimycotic preoperty has been reported. The micro propagation protocol of the medicinally important plant Bacopa monnieri was standardized using nodal segments as explants. They were surface sterilized with HgCl2 (0.1%) for 3 minutes prior to inoculation on MS media supplemented with BAP (0.5- 2.5 mg/l); IAA (0.1-0.5 mg/l for shooting,1.0-1.5 mg/l for rooting); NAA (0.1- 0.5 mg/l for shooting, 1.0-1.5 mg/l for rooting). The best performance for shoot multiplication was showed in MS medium supplemented with 1.5 mg/l BAP + 0.5 mg/l IAA. In this combination the number of shoots per explant was 16 and average length of shoot 5.54 ± 0.54 cm. But when different concentrations of NAA were applied along with 1.5 mg/l BAP the number of shoots per explant was 14 and average shoot length was 3.46 ± 0.43 on media. For root induction, best rooting was observed with half strength of MS medium supplemented with IAA (1.0 mg/l). In this combination, it was observed that the number of roots was 12 and average root length of 2.80 ± 0.09. The present study is a stepping stone for in vitro production of required active principles of Bacopa monnieri.

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