scholarly journals Stability Assessment of Cephradine Suspension Formulated in Bangladesh

2011 ◽  
Vol 3 (2) ◽  
pp. 383-391
Author(s):  
M. Z. Sultan ◽  
M. A. Mazid ◽  
M. A. Rashid
Keyword(s):  
Aqueous Medium ◽  
High Performance ◽  
Room Temperature ◽  
Chromatographic Method ◽  
Pharmaceutical Preparation ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Sodium Phosphate Buffer ◽  
The Stability ◽  
Liquid Chromatographic

Cephradine, one of the commonly used and widely prescribed antibiotics in Bangladesh, is usually formulated in the dosage forms of capsule, dry suspension and IV injection. The dry-suspension is instructed to re-disperse in pre-boiled cooled water before use. A reversed phase high performance liquid chromatographic method (HPLC) has been developed for determination of cephradine in pharmaceutical preparation. To study the stability of cephradine suspension formulated by Bangladeshi manufacturers in aqueous medium and buffer of different pHs at room temperature, a simple and rapid chromatographic method was developed using acetonitrile and monobasic sodium phosphate buffer as mobile phase in the ratio of 15:85 (v/v) over C-8 bonded silica at ambient temperature using a flow rate of 1.0 mL/min.  The study revealed that the potency of cephradine suspension was almost stable at room temperature up to 13 days in aqueous medium at pH between 4 and 5.Keywords: Cephradine; Suspension; HPLC; Potency; pH.© 2011 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.doi:10.3329/jsr.v3i2.7024                J. Sci. Res. 3 (2), 383-391 (2011)

scholarly journals An Isocratic Reversed-Phase High-Performance Liquid Chromatographic Method for the Simultaneous Determination of Benzoyl Peroxide and the Related Compounds Benzoic Acid, Benzaldehyde, Ethyl Benzoate, Methylparaben, and Propylparaben in Dermal Preparations

2008 ◽  
Vol 91 (5) ◽  
pp. 1025-1036 ◽  
Author(s):  
Mohammed Shahid Ali ◽  
Syed Rafiuddin ◽  
Muhammad Imran Munir ◽  
Mohsin Ghori ◽  
Aamer Roshanali Khatri
Keyword(s):  
Benzoic Acid ◽  
High Performance ◽  
Chromatographic Method ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Ethyl Benzoate ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic ◽  
Related Compounds

Abstract A reversed-phase high-performance liquid chromatographic method was developed for the simultaneous determination of benzoyl peroxide and the related compounds benzoic acid (BA), methylparaben, benzaldehyde, propylparaben, and ethyl benzoate. The compounds are separated on a column containing octadecyl silane chemically bonded to porous silica particles. The mobile phase is acetonitrilebuffer (45 + 55, v/v). Solutions are injected into the chromatographic system under isocratic conditions at a constant flow rate of 1.5 mL/min with UV detection at 235 nm. Analysis of stability samples showed rapid accumulation of BA by thermal degradation. A rationale has been established for the acceptable limit of BA in the formulation, which already contains BA (0.2) as a preservative. The proposed method is efficient and determines the active compound and 5 related compounds in a run time of 20 min. The method was validated according to the guidelines of the International Conference on Harmonization and demonstrated good agreement with the validation requirements.

Sign in / Sign up

Export Citation Format

Share Document