scholarly journals Direct Organogenesis and Genetic Characterization of Solanum pseudocapsicum L. in vitro Regenerated Plants

2014 ◽  
Vol 24 (1) ◽  
pp. 65-76
Author(s):  
Sayantani Nath ◽  
Anusree Das
Keyword(s):  
Genetic Characterization ◽  
Rapd Analysis ◽  
Gene Expression Pattern ◽  
Direct Organogenesis ◽  
Root Explants ◽  
Regeneration Rate ◽  
Regenerated Plants ◽  
Viable Seeds

Solanum pseudocapsicum L. is an unexplored plant of Solanaceae having horticultural and medicinal importance. Earlier the in vitro morphogenic potential of this plant species has not been explored. Induction of direct organogenesis was obtained from internodes, leaf bases and root explants from in vitro grown seedlings and plants  in MS supplemented with different concentrations and combinations of BAP (0.44 - 8.88 µM/l) and IAA (0.29 - 2.85 µM/l). Direct organogenesis was achieved in 90% of internodes, 65% of leaf bases and 75% of root explants. The optimum regeneration medium was MS supplemented with 4.44 µM/l BAP and 1.43 µM/l IAA. The regeneration rate observed six weeks after culture was approximately 20, 9 and 12 shoot buds/explants from internodes, leaf bases and root explants, respectively. cDNA RAPD analysis from organogenic stages was carried out and revealed distinct gene expression pattern. In vitro rooting in 100% shoots was achieved on MS within ten days of culture. Successfully hardened rooted plants (R0) on transfer  to the field  produced flowers, fruits and viable seeds (R1). Genetic characterization of R0 and R1 plants revealed stability and uniformity at the chromosomal and genomic DNA level. Plant Tissue Cult. & Biotech. 24(1): 65-76, 2014 (June)  D. O. I. http://dx.doi.org/10.3329/ptcb.v24i1.19201

scholarly journals Micropropagation of Tarenaya rosea (Cleomaceae) from leaf explants

Rodriguésia ◽  
2021 ◽  
Vol 72 ◽  
Author(s):  
Claudia Simões-Gurgel ◽  
Tatiana Carvalho de Castro ◽  
Cátia Henriques Callado ◽  
Lívia da Silva Cordeiro ◽  
Norma Albarello
Keyword(s):  
Large Scale ◽  
Plant Conservation ◽  
Leaf Explants ◽  
Plant Production ◽  
Anthocyanin Content ◽  
Alternative Source ◽  
Regeneration Rate ◽  
Culture Techniques ◽  
Regenerated Plants

Abstract In vitro culture techniques are recognized as efficient strategies for large-scale plant production, as well as providing alternatives for plant conservation. In this study the micropropagation of Tarenaya rosea was established using petiole and foliar blade segments cultivated on MS medium with 6-benzyladenine (BA) and/or 6-furfurylaminopurine (KIN). The regeneration rate from explants was evaluated after 30-days in culture, as well as the proliferation rate from explant-derived shoots, reached after four subcultures performed at 30-days in culture. In vitro propagation occurred by both direct (DO) and indirect (IO) organogenesis. The highest regeneration rates by DO (50% to 100%) were reached on media containing only BA, while morphogenic calluses (IO) were mainly formed with BA+KIN. Explants on media with BA showed the presence of small black nodules on their surface, and histological analysis revealed the presence of trichomes with anthocyanin content. Elongation and rooting were reached on growth regulator-free MS. Acclimatization rates around 80% were achieved and the in vitro-regenerated plants were successfully maintained under field conditions. Results show significant morphogenetic potential of T. rosea from leaf explants, mainly when cultivated in the presence of 4.4 µM BA, providing a new alternative source of plant material for biotechnological and in vitro conservation studies.

Genetic characterization of Moniliophthora perniciosa from Ecuador and in vitro sensitivity to compost tea

2019 ◽  
Vol 154 (4) ◽  
pp. 943-959
Author(s):  
María Gabriela Maridueña-Zavala ◽  
Andrea Freire-Peñaherrera ◽  
Rodrigo Fernando Espinoza-Lozano ◽  
Mirian Villavicencio-Vasquez ◽  
Maria Jimenez-Feijoo ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
Compost Tea ◽  
Moniliophthora Perniciosa ◽  
In Vitro Sensitivity

Genetic characterization of the multipotent dedifferentiated state of pigmented epithelial cells in vitro

Development ◽  
1993 ◽  
Vol 118 (4) ◽  
pp. 1025-1030
Author(s):  
K. Agata ◽  
H. Kobayashi ◽  
Y. Itoh ◽  
M. Mochii ◽  
K. Sawada ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Genetic Characterization ◽  
Pigment Cells ◽  
Intermediate Cell ◽  
Lens Cells ◽  
Myc Gene ◽  
Retinal Pigmented Epithelial Cells ◽  
Testicular Hyaluronidase

Retinal pigmented epithelial cells (PECs) of chicken embryos extensively and almost synchronously transdifferentiate into lens cells in medium containing phenylthiourea and testicular hyaluronidase, passing through the bipotent dedifferentiated state. We have isolated genes that are expressed specifically by either pigment or lens cells and analyzed their expression in the transdifferentiation process. The expression of some proto-oncogenes was also studied. In the dedifferentiation process, expression of the c-myc gene was enhanced and the transcription of PEC-specific genes (MMP115, pP344) was completely repressed. However, transcription of lens-specific genes (alpha-, beta- and delta-crystallins genes) remained silent in dedifferentiated pigment cells. Activation of len- or PEC-specific genes occurred only in conditions permissive for lens or PEC differentiation, respectively. These results indicated that lens transdifferentiation from PECs proceeds through a multipotent (or at least bipotent) intermediate cell state in which the c-myc gene is activated, but neither PEC- nor lens-specific genes are expressed.

scholarly journals Genetic characterization of paramphistomes of buffalo by HAT-RAPD analysis

2014 ◽  
Vol 38 ◽  
pp. 7-13 ◽  
Author(s):  
Giregowdanahalli PUTTALAKSHMAMMA ◽  
Umed RAMANI ◽  
Krishna SINGH ◽  
Amrutlal PATEL ◽  
Abdul PATEL ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
Rapd Analysis

scholarly journals Genetic Characterization ofStreptococcus iniaein Diseased Farmed Rainbow Trout (Onchorhynchus mykiss) in Iran

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
A. Erfanmanesh ◽  
M. Soltani ◽  
E. Pirali ◽  
S. Mohammadian ◽  
A. Taherimirghaed
Keyword(s):  
Rainbow Trout ◽  
Phylogenetic Tree ◽  
Genetic Characterization ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Streptococcus Iniae ◽  
The North ◽  
North Part ◽  
Gram Positive Cocci

Genetic characterization of strains ofStreptococcus iniaerecovered from morbidity and mortality of farmed rainbow trout in different provinces of Iran were studied. The Gram-positive cocci isolates were obtained from the kidney tissues of diseased rainbow trout on blood agar at25°Cfor 72 h. The grown bacteria were then characterized using biochemical and molecular works. The identified 26 isolates ofS. iniaeproducing a 513 bp in PCR procedure were then compared using random amplified polymorphic DNA (RAPD) analysis using 9 random primers. The phylogenetic tree of the RAPD product using UPMGA software included these strains in one genetic group but into two clusters. The results of this study show thatS. iniaestrains from the diseased rainbow trout in the north part of Iran are genetically similar to those strains in the south and west parts of the country.

scholarly journals An Uncommon Plant Growth Regulator, Diethyl Aminoethyl Hexanoate, Is Highly Effective in Tissue Cultures of the Important Medicinal Plant Purple Coneflower (Echinacea purpureaL.)

2013 ◽  
Vol 2013 ◽  
pp. 1-12 ◽  
Author(s):  
Xiao-Lu Chen ◽  
Jun-Jie Zhang ◽  
Rong Chen ◽  
Qing-Ling Li ◽  
Yue-Sheng Yang ◽  
...  
Keyword(s):  
Root Length ◽  
Primary Root ◽  
Leaf Explants ◽  
Root Weight ◽  
Root Explants ◽  
Regeneration Rate ◽  
Ploidy Levels ◽  
Purple Coneflower ◽  
Diethyl Aminoethyl Hexanoate

We investigated the effects of various concentrations of diethyl aminoethyl hexanoate (DA-6) on the regeneration and growth of adventitious buds inin vitropurple coneflower cultures. Among the 3 types of explants tested, leaf explants required higher concentrations of DA-6 than petiole and root explants in order to obtain high regeneration rates, while root explants required the lowest concentration of DA-6. Additionally, explants with higher ploidy levels were more sensitive to the addition of DA-6, while explants with lower ploidy levels required higher concentrations of DA-6 to achieve its maximal regeneration rate. Interestingly, the application of a concentration that was conducive to the regeneration of explants with lower ploidy levels was inhibitory to the regeneration of explants with higher ploidy levels. Moreover, during the growth of regenerated buds, DA-6 application significantly improved plant height and weight, root weight, root thickness, root number, primary root length, total root length, and root/top ratio. Differences in the responses of explants to supplementation with DA-6 were also observed among explants with different ploidy levels, with buds having lower ploidy levels responding to lower concentrations of DA-6. Taken together, the results of the present experiments showed that proper application of DA-6 could increasein vitroculture efficiency in purple coneflower.

scholarly journals Physical and Genetic Characterization of Deletion Mutants of Simian Virus 40 Constructed In Vitro

1977 ◽  
Vol 24 (1) ◽  
pp. 277-294 ◽  
Author(s):  
Charles N. Cole ◽  
Terry Landers ◽  
Stephen P. Goff ◽  
Simone Manteuil-Brutlag ◽  
Paul Berg
Keyword(s):  
Genetic Characterization ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
Deletion Mutants

scholarly journals Morphoagronomic characterization of Sorghum cultivar ‘CIAP 132R-05’ obtained by somatic embryogenesis in the conversion phase using different substrates

2019 ◽  
pp. 248
Author(s):  
Silvio de J. Martínez Medina ◽  
Raúl Barbón Rodríguez, Rafael Gómez-Kosky, Novisel Veitía Rodríguez ◽  
María Esther González Vega ◽  
Orlando Saucedo Castillo ◽  
Eduardo Fidel Héctor Ardisana ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Height ◽  
Somatic Embryos ◽  
Morphological Characters ◽  
Morphological Characterization ◽  
Stem Diameter ◽  
Limb Length ◽  
Regenerated Plants

Sorghum [Sorghum bicolor (L.) Moench] is an important crop after wheat, corn, rice and barley. The aim of this research was to study morphological characterization of the plant population of grain sorghum cultivar CIAP 132R-05 regenerated via somatic embryogenesis under semi controlled conditions in green house.  Plants regenerated from somatic embryos were compared to plants grown from botanical seeds. Both populations of plants were characterized morphologically, taking in account quantitative traits (plant height (cm), stem diameter, number of active leaves per plant, limb length and width, number of active roots per plant, length of the roots (cm), fresh weight (gMF), foliar area (dm2), number of shoots per plant) and qualitative traits (albino or variegated plants, color of the leaves). Quantitative characteristics of both populations corresponded with those listed in the National Register of Commercial Varieties of Cuba. However; the population of plants derived from somatic embryos showed significantly higher values ​​for plant height, stem diameter, limb length and width, which may be associated with the physiological rejuvenation produced by the effect of in vitro culture. This allowed to determine the phenotypic stability of the regenerated plants via somatic embryogenesis, by assessing morphological characters in field conditions. The results can be applied to the in vitro propagation of elite plants selected as a product of conventional breeding programs and obtained through the use of different biotechnological methods.

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